Xylariales » Xylariaceae » Astrocystis

Astrocystis bambusicola

Astrocystis bambusicola R.H. Perera & K.D. Hyde, Fungal Diversity 87: 173 (2017)

Facesofungi number: FoF 03592

Holotype: THAILAND, Doi Mae Salong, on twigs of bamboo, February 2016, R.H. Perera, MM-1 (MFLU 17-0738), ex-type living culture, MFLUCC 17-0127.

 

Saprobic on dead bamboo culms, visible as black raised spots on the host. Sexual morph: Pseudostromata 200–730 μm diam., 130–200 μm high, solitary to gregarious, scattered, superfcial, subgolobose to dome-shaped, blackened, carbonaceous, 1–3-loculate. Locules 150–250 μm diam., 140–180 μm high, subglobose, dark brown to black, immersed within pseudostroma. Peridium 25–35 μm thick, comprising several layers of dark brown to hyaline, compressed, pseudoparenchymatous cells arranged in textuta angularis, and black, fragile, carbonaceous tissue at outermost layer. Paraphyses 3.5–6 μm broad, septate, ribbon-like. Asci 96–120×5–10 μm (x̄ =108×7.5 μm, n=20), 8-spored, unitunicate, cylindric-clavate, with a short pedicel, apically rounded. Ascospores 14–16×6–8 μm (x̄ =15×7 μm, n=20), 1-seriate, ellipsoidal, aseptate, brown to dark brown, guttulate, smooth-walled, with an indistinct germ-slit. Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on PDA within 24 h, at 23–28 °C. Colonies growing on PDA, reaching 40 mm in 10 days at 23–28 °C, cottony, circular, umbonate, edge irregular, white from above and below. Mycelium superfcial to immersed in media, with hyaline, branched, septate, smooth hyphae.

 

Material examined: CHINA, Yunnan Province, Chuxiong Yi Autonomous Prefecture, Chuxiong City, Biji Village, on dead bamboo culms, 5 February 2019, H.B. Jiang, CX002 (KUN-HKAS 101769), living culture KUMCC 19-0245.Known hosts and distribution: Bamboo (Yunnan, China; Thailand) (Hyde et al. 2017).

 

GenBank Accession No: ITS=MN636698, LSU=MN636699, SSU=MN636700.

 

Notes: Astrocystis bambusicola R.H. Perera & K.D. Hyde was introduced in Hyde et al. (2017) based on a specimen collected from Thailand. Phylogenetic analyses of a combined ITS, LSU, RPB2, TUB2, SSU and TEF1-α sequence dataset showed that our strain KUMCC 19-0245 clades with the type strain of A. bambusicola (MFLUCC 17-0127) with high support (100% ML, 99% MP; Fig. 153). The new collection is morphologically close to A. bambusicola. Furthermore, our new collection has consistent base pairs with A. bambusicola (MFLUCC 17-0127) based on a comparison of ITS, LSU and SSU loci. Thus, we identify our new isolate as A. bambusicola, a species reported only in Thailand (Hyde et al. 2017); we report this species in China for the frst time.

Figure X. Astrocystis bambusicola (KUN-HKAS 101769). a Ascostromata on host surface. b Vertical section through ascostromata. c Peridium. d–g Asci. h Paraphyses. i–l Ascospores. m Germinating ascospore. n, o Culture characteristics (n=from above, o=from below). Scale bars: h=30 μm, c–g=20 μm, i–m=10 μm

 

Reference: Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Jones EB, Liu NG, Abeywickrama PD, Mapook A, Wei D, Perera RH. Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa.

 

 

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