Neoeutypella baoshanensis

Neoeutypella baoshanensis M. Raza, Q.J. Shang, Phookamsak & L. Cai, in Phookamsak et al., Fungal Diversity Notes: 10.1007/s13225-019-00421-w, [168] (2019)

Index Fungorum number: IF555372         Facesoffungi number: FoF04928

Sexual morph: Ascostromata 650–1100 µm diam., entostromatic, carbonaceous, black, solitary to gregarious, globose to long irregular in shape on host surface, erumpent through host epidermis, producing yellow pigments surrounding ascostroma. Ascomata perithecial, 500–770 high, 450–530 diam., black, immersed to semi-immersed in stromatic tissues, aggregated, globose or subglobose, ostiolate, papillate, slightly conspicuous, with periphyses. Peridium 145–250 wide, thickened unequally, two-layered, outer layer comprising 5–7 layers of thick-walled, hyaline to dark brown cells of textura angularis, inner layer comprising 3–5 layers of thin-walled, hyaline to brown cells of textura prismatica. Hamathecium 3–7 µm wide (x = 4.7 µm, n = 20), comprising aseptate, filamentous paraphyses, tapering towards the apex, embedded in hyaline gelatinous matrix. Asci (60–)75–85(–90) 9 (5.5–)6.5–7.5(–8) µm (x̄ = 77.5 9 8 µm, n = 25), 8-spored, unitunicate, spindle-shaped, long pedicellate, apically rounded

with refractive cytoplasmic strands, amyloid, with a J- , subapical ring. Ascospores (8.5–)10–11.5(–13) × (2–)2.3–2.5(–3) µm (x̄ = 10.8 × 2.4 µm, n = 50), overlapping 1–3-seriate, initially hyaline, becoming pale brown at maturity, allantoid, slightly or moderately curved, aseptate, mostly with 1–2 small guttules. Asexual morph: Colonies on natural substrate superficial, effuse, dark brown to black. Conidiophores 150–300 μm long, 4–6 μm wide, macronematous, mononematous, fasciculate, dark brown at base, slightly paler towards the apex, thick-walled, erect, straight or slightly flexuous, finely verruculose, septate, unbranched or rarely branched. Conidiogenous cells 22–38 × 3.5–8 μm (x̄= 31 × 6 μm, n = 20), polytretic, terminal, later becoming subterminal, proliferating asymmetrically, integrated, brown, finely verrucose, enlarged at apex. Conidia (10 –)16–31 × 5–9 μm (x̄= 24 × 7 μm, n = 60), solitary to catenate, when catenate in acropetal chain, fusiform to ellipsoidal, rounded at apex, truncate at base, pale brown to brown or dark brown, 3-septate, constricted at the medium septum, slightly constricted at other septa, thick-walled, verrucose. Hyphae branched, smooth, hyaline, septate, 1.5–3.5 µm diam.

Conidiophores long, branched, with phialides, mononematous, macronematous, hyaline. Conidiogenous cells (12–)14–35.5(–40) × 4–13(–15) µm (x̄ = 25 × 8.5 µm, n = 20), smooth-walled, hyaline, holoblastic, discrete, phialidic, doliiform, ampulliform or irregular in shape. Conidia (16.5–)25–37(–40) × 1.2–1.9 µm (x̄ = 29 × 1.5 lm, n = 50), filiform, solitary, aseptate, smooth-walled, unbranched,

hyaline to pale yellow.

Culture characteristics: Colonies on PDA reaching 6.5–7 mm diam. after 1 week at 25 ± 2 °C, circular, flat, slightly raised, surface dull with edge undulate, filamentous at the margin; from above, white with cotton consistency; from below, pale yellow and not producing pigment in PDA medium. Asexual morph produced on PDA after 3 weeks and colony becomes black from below.

Material examined: CHINA, Yunnan Province, Baoshan City, Longling County, on dead wood of Pinus armandii Franch. (Pinaceae), October 2015, M. Raza, HMAS 255436 (holotype), ex-type living culture, LC 12111.

GenBank Accession No: ITS = MH822887, TUB2 =MH822888.

Notes: Dendryphiella phitsanulokensis was described in Thailand on decaying wood (Hyde et al. 2018b). This is the first report of D. phitsanulokensis on Brachiaria mutica. The new collection (MFLU 18–0757) and the type material (MFLU 17–2651; Hyde et al. 2018b) are morphologically very similar. In addition, there is also no phylogenetic divergence in the strain MFLUCC 17–2242 compared to the ex-type strain of D. phitsanulokensis. Two Dendryphiella species have been so far recorded from Thailand (Liu et al. 2017c; Hyde et al. 2018b).