Xylariales » Diatrypaceae » Diatrypella

Diatrypella tectonae

Diatrypella tectonae Doilom, Q.J. Shang & K.D. Hyde, in Shang, Hyde, Phookamsak, Doilom, Bhat, Maharachchikumbura & Promputtha, Mycol. Progr. 16(4): 465 (2017)

Facesoffungi number: FoF 02403


Sexual morph: Stromata 1–1.3 mm wide, with well-developed interior, solitary to gregarious, immersed to erumpent in the bark, mostly superficial, black, glabrous, circular to irregular in shape, pustulate, bi- to multi-ascoma, with umbilicate ostioles appearing on the surface of stroma. Ascomata (excluding necks) 240–440 μm high, 255–390 μm diam. (x = 339 × 322 μm, n = 33), perithecial, immersed in stroma, delimited by a black zone in host tissues, globose to subglobose, glabrous, ostioles individual, with short neck. Ostiolar canal 105–185 μm high, 55–120 μm diam. (x = 145 × 88 μm, n = 20), cylindrical, sulcate, periphysate. Peridium 20–37 μm wide, composed of two section layers, with outer part comprising 3–6 layers of relatively small, brown to dark brown, thick-walled cells, arranged in textura angularis, inner layer comprising 3–4 layers, hyaline cells of textura angularis. Hamathecium composed of 2–2.4 μm wide, dense, filiform, septate, hyaline, paraphyses. Asci (107–)120–150(−173) × (13.5–)15.5–21.5(−30.5) μm (x = 138 × 19 μm, n = 87), spore-bearing part length [p. sp.] (80–)100–128(−146) μm (x = 114 μm, n = 97), polysporous, unitunicate, clavate, with moderately short stalks, apically rounded, with J-apical ring. Ascospores (5–)7–9(−12) × (1.5–)2–2.5(−3)μm (x = 8 × 2.3μm, n = 170), overlapping, yellowish to brown, ellipsoidal to cylindrical or elongate-allantoid, aseptate, smooth-walled. Asexual morph: Undetermined.


Culture characteristics: Ascospores germinating on PDA within 12 h. Germ tubes produced around ascospore. Colonies on PDA reaching 2.2–3.8 cm diam. after 7 days in darkness at 25 °C, colonies irregular in shape, medium dense, flat or effuse, slightly raised, with edge fimbriate, fluffy to fairly fluffy, white from above, light yellow from below, becoming white to yellow or pale brown from above and pale brown from below after 30 days; not producing pigments in agar.


Habitat: -


Known hosts: -


Known distribution: -


Material examined: Thailand, Chiang Rai Province, Mae Lao District, on dead branch of Tectona grandis Linn. (Lamiaceae), 5 February 2012, M.Doilom, MKT034 (MFLU15-3430, holotype; KUN-HKAS 95024, isotype), ex-type culture, MFLUCC 12-0172 = KUMCC 16-0023.


Notes: Diatrypella tectonae fits well with the classical description of Diatrypella. Diatrypella tectonae resembles to D. atlantica D.A.C. de Almeida, Gusmão & A.N. Mill., D. vulgaris and D. frostii Peck. However, D. tectonae can be distinguished from D. atlantica by its longer spore-bearing part of asci, as well as the black outer layer of the entostroma (de Almeida et al. 2016). Diatrypella tectonae differs from D. vulgaris and D. frostii by its yellowish inner entostroma and longer asci. Phylogenetic analyses based on ITS and β-tub sequence data show that D.tectonae (MFLUCC12-0172) form saseparate line age, but clusters with D. atlantica, D. frostii and D. vulgaris. Diatrypella species form a well-resolved clade in this study coupled with morphological differences, we introduce D. tectonae as a new species.