Diatrypella delonicis
Diatrypella delonicis R.H. Perera & K.D. Hyde, Index Fungorum 403: 1 (2019).
Index Fungorum number: IF552536 Facesoffungi number: FOF02658
Saprobic on dried seed pods of Delonix regia. Sexual morph: Stromata 0.65–1.28 mm wide, solitary or gregarious on host, immersed, with 3–4 ascomata immersed in a single stroma, comprising of outer, dark brown to black, small, tightly packed, thin parenchymatous cell layer, with inner white, loosely packed, parenchymatous cells. Ascomata 243–340 μm high, 217–380 μm diam., (x̄ = 310 × 331 μm, n = 20), perithecial, immersed in stromatic tissues, aggregated, globose to subglobose, sometimes with flattened base, pale to dark brown, carbonaceous, ostioles not prominent. Peridium 16–24 μm wide (x̄ = 18 μm, n = 20), 2 layered, inner 3–6 layers of hyaline, flattened, elongate, thin-walled cells of textura angularis, outer 4–7 layers of pale to dark brown, flattened, thick-walled cells of textura angularis. Hamathecium comprising 8.2–13 μm wide (x̄ = 9.8 μm, n = 20), septate, paraphyses, longer than the asci, tapering towards the apex, wider at the base. Asci 95–100 × 14–20 μm (x̄ = 100 × 18 μm, n = 25), multi-spored, unitunicate, clavate, with narrow, long, thin-walled pedicel, with swollen upper portion, apex rounded, without a visible apical apparatus. Ascospores 6.2–7.4 × 1.4–1.8 μm (x̄ = 6.7 × 1.6 μm, n = 20), overlapping, pale brown, allantoid to cylindrical, unicellular, with small, fat globules at the ends, smooth-walled, without sheath or appendages. Asexual morph: Undetermined.
Culture characters: Ascospores germinated on PDA within 18 h and germ tube produced from one or both ends of the ascospore. Reaching 6.5 cm within 14 days on PDA, at 25 °C, colonies medium dense, circular, flat, with diffuse margin, white, below similar in color, not zonate, not producing pigmentation on PDA media.
Habitat: on dried seed pods of Delonix regia
Known distribution: Thailand
Material examined: THAILAND, Chiang Rai Province, Muang District, Mae Fah Luang University, in front of S7 building, on dried seed pods of Delonix regia (Boj. ex Hook.) Raf. (Fabaceae), 10 December 2014, R.H. Perera, RHP 75 (MFLU 16-1016, holotype), ex-type living culture (MFLUCC 15-1014); ibid. RHP 77 (MFLU 16-1032).
GenBank numbers: ITS: MH812994, LSU: MH812996, TUB2: MH847790 (MFLUCC 15-1014); ITS: MH812995, LSU: MH812997, TUB2: MH847791 (MFLU 16-1032).
Notes: Diatrypella delonicis clusters with D. major, D. frostii and D. vulgaris within Diatrypella sensu stricto. DNA sequences of D. delonicis and putative strain of D. major (1058) differed in 2 nucleotides in the ITS region. DNA sequences of D. delonicis and putative strain of D. frostii (UFMGCB 1917) differed in 10 nucleotides in the ITS region. Sequence data of the TUB2 region of D. major and D. frostii are not available in the GenBank for the comparison. Diatrypella delonicis and ex-type strain of D. vulgaris (HVGRF03) showed 1 nucleotide difference in the ITS region, and 14 different nucleotides in the TUB2 region. DNA sequences of D. delonicis and ex-type strain of D. tectonae (MFLUCC 12-0172) differed in 7 nucleotide positions of the ITS region. DNA sequence data of D. heveae (ex-type MFLUCC 17-0368) differed in 11 nucleotides in the ITS region and 33 in the TUB2. A key to species is provided above including the phylogenetically closely related species and the taxa were described from Thailand, D. heveae and D. tectonae.
Figure X. Diatrypella delonicis (MFLU 16-1016, holotype). a Herbarium material. b Appearance of ascomata on host substrate. c Longitudinal section through stroma with ascomata. d Vertical section through ascomata. e Close up of the peridium. f Paraphyses. g–i Asci. j Ascospores. k Germinating ascospore. l, m Colony on PDA (l from above view, m from below view). Scale bars: b = 1 mm, d = 500 μm, e–i, k = 20 μm, j = 10 μm
Reference:
Hyde KD, Tennakoon DS, Jeewon R et al. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers 96: 1–242.
Recent Genus
AmphisphaeriaParaeutypella
Halorosellinia
Recent Species
Torula chromolaenaePericonia delonicis
Neopestalotiopsis theobromicola