Tubeufiales » Tubeufiaceae » Kamalomyces

Kamalomyces mangrovei

Kamalomyces mangrovei Dayarathne & K.D. Hyde, in Hyde et al., Fungal Diversity: 10.1007/s13225-019-00429-2, [93] (2019)

Index Fungorum number: IF555385         Facesoffungi number: FoF04946

 

Sexual morph: Ascomata 280–315 × 250–300 µm, superficial, solitary to gregarious, embedded in a subiculum of crowded, black, septate, thick-walled hyphae, superficial, solitary, gregarious, globose to subglobose, glabrous, short-stalked, apapillate, with indistinct ostiolate. Peridium 30–45 µm wide, comprising light brown cells of textura anguaris, and inwardly small, subhyaline cells of textura prismatica. Hamathecium comprising numerous, 1.5–2 µm wide, filiform, septate, branched, hyaline pseudoparaphyses. Asci 148–180 × 16–20 µm (x̄ = 165 × 18 µm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, short pedicellate, apically rounded, with an ocular chamber. Ascospores 52–67 × 6.5–8.5 µm (x̄ = 62 × 7.5 µm, n = 30), 2–3-seriate, hyaline becoming light brown when mature, elongate cylindrical to fusiform-clavate, tapering towards the lower cells, enlarged at the 4th and 5th cell, straight or slightly curved, 8–9 septa, distoseptate. Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA reaching 3 cm diam. after 30 days at 25 °C, circular, smooth margin white at first, dark gray to black after 6 weeks, flat on the surface, without aerial mycelium, reverse brownish black.

 

Host and Habitat: Saprobic on decaying submerged wood in a mangrove stand.

 

Known distribution: Thailand.

 

Material examined: THAILAND, Ranong Province, Maung District, Mu 4 Tombol Ngao, Ranong Mangrove Research Center (GPS: 9°430 to 9°570N; 98°290 to 98°390E), on decaying submerged wood of mangrove, 7 December 2016, M.C. Dayarathne, MCD 053 (MFLU 18-1691, holotype), ex-type living culture MFLUCC 17-0407, TBRC.

 

GenBank Accession No: ITS: MH878781, LSU: MH878779, SSU: MH878796, TEF1: MH886508.

 

 

Figure X. Kamalomyces mangrovei (MFLU 18-1691, holotype). a, b Ascomata. c Section of ascoma. d Section through peridium. e–g Asci and pseudoparaphyses. h–k Ascospores. l Germinating ascospore. m, n Cultures on PDA (m from above view, n from below view). Scale bars: a = 500 µm, c = 200 µm, b = 100 µm, e–g = 50 µm, d, h–l = 20 µm.

 

Notes: Kamalomyces mangrovei is characterized by solitary to gregarious, globose to subglobose, short-stalked ascomata, lacking ostioles embedded in a subiculum of black hyphae, bitunicate, cylindrical to clavate asci and hyaline, elongate cylindrical to fusiform-clavate, septate ascospores. Kamalomyces mangrovei is morphologically best fit Kamalomyces (Verma et al. 2008; Dubey and Neelima 2013; Boonmee et al. 2011, 2014; Lu et al. 2018; Phookamsak et al. 2018). Kamalomyces mangrovei closely resembles K. bambusicola Y.Z. Lu & K.D. Hyde and K. thailandicus Phook., Y.Z. Lu & K.D. Hyde due to its ascomatal characters, asci and ascospore shape. However, K. mangrovei differs significantly from K. bambusicola and K. thailandicus by having fewer ascospore septa (8–9 vs 27–30 and 33–36 septa) and being swollen at the 4th and 5th cells. The novel species occurred on a mangrove species while K. bambusicola and K. thailandicus have been reported from bamboo. This is the first record of Tubeufiaceae from a marine habitat (Jones et al. 2015, 2019). According to our phylogenetic analyses with concatenated LSU, ITS and TEF1 sequence data, K. mangrovei clustered within the genus Kamalomyces with high statistical support (Hyde et al. 2019).

 

Reference:

1. Boonmee S, Zhang Y, Chomnunti P, Chukeatirote E, et al. (2011) Revision of lignicolous Tubeufiaceae based on morphological reexamination and phylogenetic analysis. Fungal Divers 51:63–102

2. Boonmee S, Rossman AY, Liu JK, Li WJ, et al. (2014) Tubeufiales, ord. nov., integrating sexual and asexual generic names. Fungal Divers 68: 239–282.

3. Dubey R, Neelima AM (2013) Kamalomyces mahabaleshwarensis sp. nov. (Tubeufiaceae) from the western Ghats, India. Mycosphere 4: 760–763

4. Hyde KD, Tennakoon DS, Jeewon R, Bhat DJ et al. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers. 96: 1–242.

5. Jones EBG, Pang KL, Abdel-Wahab MA, Scholz B, et al. (2019) An online resource for marine fungi. Fungal Divers 96: 347–433

6. Jones EBG, Suetrong S, Sakayaroj J, Bahkali AH, et al. (2015) Classification of marine Ascomycota, Basidiomycota, Blastocladiomycota and Chytridiomycota. Fungal Divers 73: 1–72

7. Lu YZ, Liu JK, Hyde KD, Jeewon R, et al. (2018) A taxonomic reassessment of Tubeufiales based on multi-locus phylogeny and morphology. Fungal Divers 92:131–344

8. Phookamsak R, Lu YZ, Hyde KD, Jeewon R, et al. (2018) Phylogenetic characterization of two novel Kamalomyces species in Tubeufiaceae (Tubeufiales) Mycol Prog 17: 647–660

9. Verma RK, Sharma N, Soni KK (2008) Forest fungi of Central India. Jamaluddin 11–418

 

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