Phaeoisaria siamensis
Phaeoisaria siamensis Jayasiri & K.D. Hyde, in Hyde et al., Fungal Diversity: 10.1007/s13225-019-00429-2, [156] (2019)
Index Fungorum number: IF555710 Facesoffungi number: FoF05324
Sexual morph: Undetermined. Asexual morph: Conidiomata scattered, indeterminate, erect, rigid, superficial, dark brown composed of compact appressed conidiophores. Conidiophores 330–380 μm high, 20–25(–30) μm (x̄ = 360 × 24.5 μm, n = 20) wide, macronematous, in synnematous conidiomata, scattered, synnemata subulate or cylindrical, indeterminate, at the base 13–15 μm beneath the fertile portion with conidiogenous cells, composed of medium to dark brown, smooth, septate parallel hyphae, 2–2.5(–3) μm wide, splaying out at the middle to apex. Conidiogenous cells 8–12 × 2–2.5 μm (x̄ = 10.5 × 2.3 μm, n = 20), integrated, terminal and intercalary, polyblastic, sympodial, cylindrical or somewhat attenuated toward the tip, pale brown near base, subhyaline at the apex, denticulate, denticles conspicuously cylindrical, 2–2.5 × 1–1.5 μm. Conidia 5–8 × 3–4 μm (x̄ = 6.2 × 3.5 μm, n = 30), globose to subglobose, hyaline, aseptate.
Culture characteristics: Conidia germinated on MEA. Colonies are appressed, circular, flat surface, edge entire, first cream then become dark brown and rise in the centre with mycelium, reverse brown reaching 10 mm in 2 weeks at 18 °C.
Host and Habitat: Saprobic on Meliaceae sp.
Known distribution: Thailand.
Material examined: THAILAND, Chiang Mai Province, Doi Suthep, 22 December 2015, decaying fruits of Meliaceae sp., Subashini C. Jayasiri, C 128 (MFLU 16-0953, holotype), ex-type living culture MFLUCC 16-0607.
GenBank Accession No: ITS: MK607610, LSU: MK607613, RPB2: MK607611, SSU: MK607612.
Figure X. Phaeoisaria siamensis (MFLU16-0953, holotype). a Host fruit. b, c Conidiomata on host surface. d Conidiophore. e–g Conidiogenous cells arranged on conidiophores. h Conidia. Scale bars: d = 100 μm, e–g = 10 μm.
Notes: Phaeoisaria siamensis fits with the generic description of Phaeoisaria in having indeterminate synnemata with aseptate ellipsoidal, obovoidal, fusiform-cylindrical or falcate conidia formed on a sympodially extending rachis. Phaeoisaria siamensis (MFLUCC 16-0607) forms a sister clade to P. aquatica (MFLUCC 16-1298) with high statistical support (99% MLBS/1.0 BYPP; Hyde et al. 2019). Phaeoisaria aquatica differs from P. siamensis in having compactly and parallelly adpressed conidiophores, with flared conidiogenous cells in the above half (Luo et al. 2018). A comparison of the ITS and RPB2 nucleotides of these two strains reveals 8 (1.6%) and 13 (1.5%) nucleotide differences which justifies the new species following the guidelines of Jeewon and Hyde (2016).
Reference:
1. Hyde KD, Tennakoon DS, Jeewon R, Bhat DJ et al. (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Divers 96: 1–242.
2. Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677.
3. Luo ZL, Hyde KD, Liu JK, Bhat DJ, et al. (2018) Lignicolous freshwater fungi from China II: novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461.
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