Pleosporales » Phaeosphaeriaceae » Wojnowiciella

Wojnowiciella kunmingensis

Wojnowiciella kunmingensis Phookamsak, Wanas. & K.D. Hyde, in Phookamsak et al., Fungal Diversity: 10.1007/s13225-019-00421-w, [60] (2019)

Index Fungorum number: IF556170         Facesoffungi number: FoF05704


Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 110–190 μm high, 110–190 μm diam., pycnidial, scattered, solitary to gregarious, immersed, slightly raised, visible as small black dot on host surface, uni-loculate, globose to subglobose, glabrous, dark brown to black, ostioles central, apapillate, with pore-like opening. Conidiomata walls 5–12 μm wide, thin-walled, of equal thickness, composed of 1–3 cell layers, of flattened, dark brown, pseudoparenchymatous cells, arranged in a textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells (3–)4–6.5(–9) × (2.5–)3.5–6(–8) μm l/w ( = 5 × 5.3 μm, n = 40), holoblastic, phialidic, discrete, determinate, ampulliform to doliiform, unbranched, aseptate, hyaline, smooth, arising from the inner cavity of the conidioma wall. Conidia (16–)18–24(–27.5) × 4–6(–7) μm l/w ( = 22 × 5.6 μm, n = 50), dark brown, subcylindrical to fusiform, or reniform, slightly curved, with acute to rounded apex, and rounded to truncate base, 3–7-septate, not constricted at the septa, thick-walled, smoothwalled, with guttules, having flattened, mucous caps at both ends.


Culture characteristics: Colonies on PDA reaching 35–38 mm diam. after 2 weeks at room temperature

(20–30 C). Colony medium dense, slightly irregular in shape, flattened to slightly raised, surface slightly rough with greyish-green hyphal tufts, edge undulate, with entire margin, floccose; from above, slightly radiating, white to cream at the margin, pale brown at the middle, separated from the margin with brown to dark green concentric ring near the edge, greyish green to dark green at the centre; from below, white to cream margin, brown to dark brown at the centre; not producing pigmentation on agar medium.


Material examined: CHINA, Yunnan Province, Kunming City, Kunming Institute of Botany, on dead hanging branches of Lonicera maackii, 20 April 2017, R. Phookamsak, KIB031 (KUN-HKAS 102233, holotype), ex-type living culture, KUMCC 18-0159 (KIB031A), KUMCC 18-0160 (KIB031B).


GenBank Accession No: ITS: MN582743, LSU: MK356354, SSU: MK356368, TEF1-a: MK359071

(KUMCC 18-0159); ITS: MK356381, LSU: MK356355, SSU: MK356369, TEF1-a: MK359072, RPB2: MK359078 (KUMCC 18-0160).


Notes: In the NCBI BLASTn search of ITS sequences, Wojnowiciella kunmingensis most closely matches W. dactylidis (Wijayaw. et al.) Hern.-Restr. & Crous, W. spartii (W.J. Li et al.) Hern.-Restr. & Crous and Wojnowicia italica Qing Tian et al. with 99% similarity. ITS and TEF1-a genes could not resolve the novelty of the Wojnowiciella species in this study. A comparison of ITS and TEF1-a nucleotide bases shows that Wojnowiciella kunmingensis is not significantly different from W. dactylidis and other Wojnowiciella species as well as Wojnowicia italica. However, a comparison of RPB2 sequence data shows that Wojnowiciella kunmingensis differs from W. dactylidis and Wojnowicia italica in 21/856 bp (2.4%) and 20/954 bp (2.1%), respectively.

Multigene phylogenetic analyses based on a combined LSU, SSU, TEF1-a and ITS sequence data show that Wojnowiciella kunmingensis forms a sister lineage with W. dactylidis and clusters with other Wojnowiciella species. However, W. kunmingensis differs from W. dactylidis in having 3–7-septate conidia, while W. dactylidis has 7–11-septate conidia (Liu et al. 2015a). Wojnowiciella leptocarpi Crous et al. also has 3–7-septate conidia, with mucous caps at both ends. However, W. kunmingensis has longer conidia ((16–)18–24(–27.5) × 4–6(–7) μm versus 26–36 × 4–6 μm; Hernandez-Restrepo et al. 2016), that are brown to dark brown, and smooth-walled. The conidia of W. leptocarpi are orange brown, thick-walled, and verruculose (Hernandez-Restrepo et al. 2016). A comparison of RPB2 sequence data shows that W. kunmingensis differs from W. leptocarpi in 29/851 bp (3.4%). Wojnowiciella lonicerae (Wijayaw. et al.) Hern.-Restr. & Crous was also collected from Lonicera, but it has 8–11-septate conidia (Liu et al. 2015a).


Figure X. Wojnowiciella kunmingensis (KUN-HKAS 102233, holotype). a Appearance of conidiomata on host. b Section through conidioma. c Section through conidioma wall. d–g Conidiogenous cells. h–k Conidia. l Germinated conidium. Scale bars a = 200 µm, b = 50 µm, c, i = 20 µm, h, j–l = 10 µm, d–g = 5 µm.


Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, et al. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity (2019) 95:1–273




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