Pleosporales » Phaeosphaeriaceae » Ophiosphaerella

Ophiosphaerella chiangraiensis

Ophiosphaerella chiangraiensis Phookamsak & K.D. Hyde, in Yuan et al., Fungal Diversity: 10.1007/s13225-020-00461-7, [29] (2020)

Index Fungorum number: IF556471; Facesoffungi number: FoF 06128


Sexual morph: Ascomata 190–370 μm high (including papilla), 250–460 μm diam, scattered, solitary, semi-immersed, visible as black, shiny dot on the host surface, globose to subglobose, uniloculate, glabrous, ostiole central, with minute papilla, filled with hyaline periphyses. Peridium 10–20 μm wide, of equal thickness, composed of several layers of small, flattened to broad, dark brown pseudoparenchymatous cells, paler towards the inner layers, arranged in textura angularis to textura prismatica. Hamathecium composed of numerous, 1.8–3.8 μm wide, filamentous, septate pseudoparaphyses, anastomosing above the asci, embedded in a hyaline gelatinous matrix. Asci (125–)135–160(–180) × (6.5–)7–10 μm, (x̄ = 152.5 × 8.6 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical to subcylindric-clavate, subsessile to short pedicellate, with obtuse to furcate pedicel, apically rounded, with well-developed ocular chamber, clearly seen at immature state. Ascospores (122–)140–160(–165) × 2–3(–4) μm, (x̄ = 143.6 × 2.7 μm, n = 30), overlapping, in spiral, filiform, tapering towards the lower cell, pale yellowish, curved, (14–)15–16(–18)-septate, not constricted at the septa, smooth-walled, lacking a mucilaginous sheath. Asexual morph: Undetermined.

Culture characteristics: Colonies on PDA reaching 38–40 mm diam after 2 weeks at room temperature (20–30 °C). Colony medium dense, circular, flattened, slightly raised, surface smooth, with entire edge, floccose; from above, white to cream; from below, cream to pale yellowish; not producing pigmentation on agar medium.

Material examined: THAILAND, Chiang Rai Province, Muang District, Ta Sai, on dead culm of grass, 30 June 2011, R. Phookamsak, RP0130 (MFLU 11–0246, holotype), extype living culture, MFLUCC 12–0007.

GenBank Accession No: ITS: KM434272; LSU: KM434282; SSU: KM434291; TEF1-α: KM434300; RPB2: KM434308.


Notes: Phylogenetically, strain MFLUCC 12–0007 formed a sister lineage with Ophiosphaerella agrostidis (MFLUCC 11-0152) with full support (100% ML, 100% MP). Comparisons of ITS and TEF1-α sequences show that MFLUCC 12-0007 differs from O. agrostidis (MFLUCC 11-0152) in 11/494 bp (2.2%) of ITS and 22/878 bp (2.5%) of TEF1-α. Therefore, we introduce MFLUCC 12–0007 as a new species, O. chiangraiensis. O. chiangraiensis is similar to O. agrostidis (see description of this species in Câmara et al. 2000 and Phookamsak et al. 2014) in having filiform, pale yellowish, (14–)15–16(–18)-septate ascospores. However, O. chiangraiensis differs from O. agrostidis by having slightly larger ascomata and ascospores, and smaller asci (Câmara et al. 2000).

Figure 3. Ophiosphaerella chiangraiensis (MFLU 11–0246, holotype). a Appearance of ascomata on host surface. b, c Sections through ascomata. d, e Section through peridium. f Pseudoparaphyses stained with Congo Red. g, h Asci. i Fissitunicate asci stained with Congo Red. j–m Ascospores. Scale bars: a = 200 μm, b, c = 100 μm, d = 50 μm, e–m = 20 μm




Yuan HS, Lu W, Dai YC, Hyde KD et al. 2020 Fungal diversity notes 1277–1386: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity volume 104:1–266.


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"The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants Rhododendron species and Dracaena species"

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