Pleosporales » Phaeosphaeriaceae » Neosetophoma

Neosetophoma lonicerae

Neosetophoma lonicerae Phookamsak, Wanas. & K.D. Hyde, in Phookamsak et al., Fungal Diversity: 10.1007/s13225-019-00421-w, [54] (2019)

Index Fungorum number: IF556168         Facesoffungi number: FoF05702

 

Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 110–160 μm high, 80–160 μm diam., pycnidial, scattered, solitary to gregarious, immersed to semi-immersed, uniloculate, globose to subglobose, glabrous, dark brown to black, visible as small black dot on the host surface, associating with other fungal taxa, ostiole central, occasionally near the centre, minutely mammiform papilla. Conidiomata walls 5–12 μm wide, equally thin-walled, composed of 2–3 cell layers, of broad, brown to dark brown pseudoparenchymatous cells, arranged in a textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 3.5–7 × (3–)5–10 μm (x̅ = 6.2 × 6.7 μm, n = 20), enteroblastic, phialidic, discrete, determinate, ampulliform to doliiform, unbranched, aseptate, hyaline, smooth, minute collarette with periclinal wall thickening, arising from the inner cavity of the conidioma wall. Conidia (8.5–)9–12(–14) × 4–5 μm (x̅ = 11 × 4.8 μm, n = 50), yellowish brown, ellipsoidal, 1–3-septate, not constricted at the septa, smooth-walled, lacking mucilaginous sheath surrounding conidia.

 

Culture characteristics: Colonies on PDA reaching 33–35 mm diam. after 3 weeks at room temperature (20–30 ºC). Colony medium dense, circular, flattened to raised, surface slightly rough with hyphal tufts, edge entire, velvety to fluffy; from above, white to white yellowish at the margin, light green to yellowish green at the centre; from below, radiating outwards colony, white to cream at the margin, dark green to black at the middle, orangish brown at the centre; producing yellowish pigment on agar medium.

 

Material examined: CHINA, Yunnan Province, Kunming City, Kunming Institute of Botany, on dead hanging branches of Lonicera maackii, 20 April 2017, R. Phookamsak, KIB033 (KUN-HKAS 102231, holotype), ex-type living culture, KUMCC 18-0155 (KIB033A), KUMCC 18-0156 (KIB033B).

 

GenBank Accession No: ITS: MK356375, LSU: MK356349, SSU: MK356363, TEF1-a: MK359067 (KUMCC 18-0155); ITS: MK356376, LSU: MK356350, SSU: MK356364, TEF1-a: MK359068 (KUMCC 18-0156).

 

Notes: In the NCBI BLASTn search of ITS sequence, Neosetophoma lonicerae has a closest match with fungal endophyte species (M16-3161) with 100% similarity and is closely related to N. italica W.J. Li et al., N. rosarum R.H. Perera et al., N. samarorum (Desm.) Gruyter et al. and N. rosigena Wanas. et al. with 98% similarity. Multigene phylogenetic analyses based on a combined LSU, SSU, TEF1-a and ITS sequence dataset show that N. lonicerae forms a separate lineage, clustering with N. rosigena, N. samarorum and N. garethjonesii Tibpromma et al. with moderate support (76% ML and 0.98 BYPP). A comparison of ITS nucleotide base pairs shows that N. lonicerae differs from N. rosigena, N. samarorum and N. garethjonesii in 14/555 bp (2.5%), 15/555 bp (2.7%) and 16/522 bp (3%), respectively. We therefore, introduce N. lonicerae as a new species following the guidelines of Jeewon and Hyde (2016). Neosetophoma lonicerae is similar to some other Neosetophoma species in having pale brown to brown, oblong to ellipsoidal, or subfusoid, 1–3-septate conidia; such as in N. iranianum Papizadeh et al., N. italica, N. rosae R.H. Perera et al., N. rosarum and N. shoemaker Senwanna et al. However, they can be distinguished based on their conidial size, host occurrence and phylogenetic distance (Liu et al. 2015a; Karunarathna et al. 2017; Hyde et al. 2018b; Wanasinghe et al. 2018).

Figure X. Neosetophoma lonicerae (KUN-HKAS 102231, holotype). a Appearance of conidiomata on host. b Section through conidioma. c, d Section through conidioma wall. e–h Conidiogenous cells. i–n  Conidia. o Germinated conidium. p, q Culture characteristics on PDA (p = from above, q = from below). Scale bars a = 200 μm, b = 50 μm, c–h, o = 10 μm, i–n = 5 μm.

 

Reference:

Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, et al. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity (2019) 95:1–273

 

 

 

 

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