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Sarcopodium vanillae

Sarcopodium vanillae

Sarcopodium vanillae (Petch) B. Sutton,

Trans. Brit. Mycol. Soc. 76: 99 (1981)

≡ Actinostilbe vanillae Petch, Ann. Roy. Bot. Gard. (Peradeniya) 9(3):327 (1925)

 

Sexual morph: Ascomata 150–200 μm high, 160–240 μm wide (x = 173 × 189 μm, n = 5), perithecial, subglobose, solitary or in groups, soft-textured, pale yellow or rarely orange, superficial on a leaf or erumpent, with a papillate ostiole. Peridium 15–25 μm wide (x = 47 μm, n = 5), composed of several layers of white to light orange cells of textura angularis. Asci 36–52 × 3–5 μm (x = 44 × 4.5 μm, n = 20), 4-spored, unitunicate, cylindrical, rounded at apex, clavate to fusiform, short pedicellate. Ascospores 8–12 × 3–4.5 μm (x = 11 × 3.9 μm, n = 20), fasciculate, broadly elongate, 1-septate. Asexual morph: Mycelium, branched, septate, hyaline, smooth. Conidiomata 200–210 × 220–240 μm (x = 205 × 230 μm, n = 5), sporodochial, solitary or gregarious, setiferous, yellow to bright yellow or rarely orangish brown, soft-textured, superficial, separate, gregarious or confluent, sessile, attached to the substratum by a small stroma concentrated in the epidermis or outer layers of peridermal tissue, pulvinate, setose. Setae 100–110 × 5–10 μm (x = 105 × 7.5 μm, n = 5), septate, unbranched, cylindrical, incurved, erect, very thick-walled, medium to pale golden brown, more or less straight (occasionally slightly bent either at the base or nearer the apex), erect, hyaline, pointed or rounded at the apex. Conidiophores mononematous, verticillately or penicillately branched, straight or flexuous, smooth, hyaline, short, septate, with 1–4 monochasial branching, compactly arranged, cylindrical, intermixed with long setae. Conidiogenous cells enteroblastic, monophialidic, integrated, cylindrical or more frequently tapered towards the apices, subulate, widest from middle to base, 10–20 × 1.2–2.7 μm (x = 15 × 1.95 μm, n = 5), with inconspicuous collarette, hyaline, smooth, formed as the ultimate branches of conidiophores and completely covering the external face of the conidiomata. Conidia 5–9 × 2.1–2.6 μm (x = 6 × 2.5 μm, n = 20), cylindrical, 0–1-septate, smooth, hyaline, rounded at both ends, held together in a slimy mass, ellipsoid to oval, straight.

Notes: Our strains share similar morphological characters with S. vanillae strain CBS 100852, which was isolated from Anthurium sp. in Ecuador (Lombard & al. 2015). The phylogenetic analysis also supports the close relationship. The genus Sarcopodium has both sexual and asexual morphs (Wijayawardene & al. 2017a,b, 2018). The conidial morphology of our strain is similar to S. circinatum (the type species of the genus); however our conidiomata and conidiophores more closely resemble Volutella ciliata (CBS 483.61) (Lombard & al. 2015). Previously, only the asexual morph has been observed for S. vanillae (Sutton 1981), and this study is the first report of the sexual morph. Sarcopodium vanillae has been reported from Abelmoschus manihot in Papua New Guinea, Citrus nobilis in Brunei, Vanilla planifolia in Sri Lanka, and V. tahitensis in Papua New Guinea (Farr & Rossman 2019). This study provides the first report of S. vanillae from Dracaena, and its first report from Thailand.