Nigrograna magnoliae
Nigrograna magnoliae Wanas., PLoS One 15 (7, e0235855): 10 (2020)
Index Fungorum number: IF557331; Facesoffungi Number: FoF09092
Saprobic on dead twigs of Castanopsis indica. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 140–250 μm high × 110–140 μm diam., (x̅ = 200 × 120 μm, n = 5), scattered, immersed to erumpent, formed a dome shaped areas on the host surface, black, unilocular, coriaceous, subglobose or obpyriform, with central ostioles. Conidiomata wall 15–30 μm thick, of unequal thickness, composed of dark brown outer layers and inner layers comprising brown, thick walled cells of textura angularis. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 4.7–6.3 × 1.5–2.6 μm (x̅ = 5.6 × 2.1 μm, n = 10), enteroblastic, phialidic, determinate, discrete, cylindrical, hyaline, smooth walled, arising from the stratum. Conidia 3–4 × 1.4–1.8 (x̅ = 3.5 × 1.8, n = 30) μm, straight, hyaline, oblong, one celled, aseptate, ends rounded, smooth walled, guttulates.
Culture characteristics: Colonies on PDA, reaching 30 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, dense, flat, umbonate, surface rough with undulate edge, white, velvety; reverse, dark brown at the center, white at the margin.
Material examined: China, Yunnan Province, Lancang, Lahu Autonomous Prefecture, Hani, on dead woody twigs of Castanopsis indica (Fagaceae), 19 July 2020, G.C. Ren, LGY01 1 (HKAS 122720), living culture KUMCC 21 0542.
Known distribution:Saprobic on dead twigs of Magnolia denudate (Magnoliaceae) in China (Wanasinghe et al. 2020b), on submerged wood in Thailand (Zhang et al. 2020a), dead twigs of Magnolia grandiflora (Magnoliaceae) in Thailand (https://www.ncbi.nlm.nih.gov/nuccore/MN081891.1), on dead twigs of Castanopsis indica (Fagaceae) in China
GenBank number: SSU: OQ168217, LSU: OQ170864, ITS: OQ158942, tef1-α: OR613438, rpb2: OR578561.
Notes: In the phylogenetic analysis of the present strain KUMCC 21-0542 clustered with N. magnoliae (GZCC 17-0057) with 87% ML bootstrap support and 1.00 BYPP value. Nigrograna magnoliae was introduced by Wanasinghe et al. (2020b) based on the combined phylogeny of SSU, LSU, rpb2, and tef1-α sequence data. Our asexual collection (KUMCC 21-0542) has no significant differences with the type species of N. magnoliae (MFLUCC 20–0020) (Wanasinghe et al. 2020b). Based on morphological characteristics and phylogenetic analysis, we report our isolation as a new host record of N. magnoliae from decaying wood of Castanopsis indica in China and provide the asexual morph for N. magnoliae. Furthermore, the sequence data of N. aquatica (MFLUCC 14-1178) was a direct submission without any publication, and we found that this species clustered with N. magnolia in our multi-gene phylogeny, while the type of N. aquatica (MFLUCC 17-2318) clustered with N. locuta-pollinis. However, the comparison of SSU, LSU, ITS, rpb2 and tef1-α sequence data reveals there is no significant difference between N. aquatica (MFLUCC 14-1178) and N. magnoliae (MFLUCC 20–0020). Therefore, we suspect that the author may have misidentified the N. aquatica when submitting the strain MFLUCC 14-1178 sequence.
Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, tef1-α, and rpb2 sequence data, representing Nigrograna (Nigrogranaceae). Related sequences are obtained following de Lu et al. (2022b) and Boonmee et al. (2021). Forty-six strains are included in the combined analyses, which comprise 4194 characters for SSU, LSU, ITS, tef1-α, and rpb2 alignment. Seriascoma didymospora (MFLUCC 11-0194, MFLUCC 11-0179) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -17623.075885 is presented. The matrix had 1076 distinct alignment patterns, with 31.53% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.248028, C = 0.248350, G = 0.266441, T = 0.237180; substitution rates AC = 1.506227, AG = 4.589175, AT = 1.553542, CG = 1.033267, CT = 10.958332, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.
Figure 2. Nigrograna magnolia (HKAS 122720). a Material examined. b Appearance of conidiomata on host substrate. c Sections through conidiomata. d Conidiomatal wall. e, f Conidiogenous cells and developing conidia. g‒i Conidia. j Germinated conidium. k, l Culture characters on PDA (k = from above, l = from below). Scale bars: c = 50 μm, d, j = 20 μm, e‒g = 10 μm, h, i = 5 μm, k, l = 30 mm.
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