Nigrograna kunmingensis
Nigrograna kunmingensis T.Y. Du & Tibpromma Fungal Diversity (2023)
Index Fungorum number: IF559865; Facesoffungi Number: FoF12956
Saprobic on dead twigs of Castanopsis mekongensis. Sexual morph: Ascomata 97–160 μm high, 150–320 μm diam., (x̅ = 133 × 213 μm, n = 6), immersed under a small blackened pseudoclypeus, appearing as black, circle regions on the host surface, solitary or gregarious, subglobose or elliptical to ampulliform, dark brown, with an ostiole. Peridium 8–16 μm wide, 2–5-layered, composed of dark brown cells of textura angularis. Hamathecium 1.3–2.5 μm wide, comprising numerous, filamentous, branching, septate, hyaline pseudoparaphyses. Asci 70–97 × 9.5–14 μm (x̅ = 84 × 12 μm, n = 20), 3–8-spored, bitunicate, fissitunicate, clavate to cylindric-clavate, slightly curved, short pedicellate, apically rounded, with a minute ocular chamber when immature. Ascospores 14–17 × 5.5–7.5 μm (x̅ = 15.6 × 6.3 μm, n = 30), overlapping 1–2-seriate, broadly fusiform to ellipsoid, straight, initially yellowish brown with 1-septate, becoming to brown with 3-septate when mature, the upper cell is slightly wider than the lower cell, slightly constricted at the primary median septum, guttulate. Asexual morph: Undetermined.
Culture characteristics: Colonies on PDA, reaching 30 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, dense, flat, umbonate, surface rough with undulate edge, white, velvety; reverse, dark brown at the center, white at the margin.
Material examined: China, Yunnan Province, Lancang, Lahu Autonomous Prefecture, Hani, on dead woody twigs of Castanopsis mekongensis (Fagaceae), 20 July 2020, G.C. Ren, LGY13 (HKAS 122729), living culture KUMCC 21-0551.
Known distribution: On dead stems of Gleditsia sinensis (Fabaceae) and dead twigs of Castanopsis mekongensis in China.
GenBank numbers ‒ SSU: OQ168219, LSU: OQ170866, ITS: OQ158944, tef1-α: OR613436.
Notes ‒ Nigrograna kunmingensis was introduced by Liu et al. (2024) based on the combined phylogenetic analyses of SSU, LSU, ITS, rpb2, and tef1-α sequence data. In the present study, the multi-gene phylogeny indicates that our strain (KUMCC 21-0551) and other existing strains of N. kunmingensis (ZHKUCC 22-0242, ZHKUCC 22-0243) group together and formed a monophyletic clade with 100% ML bootstrap support and 1.00 BYPP value. Our collection (KUMCC 21-0551) have no significant differences with the type species of N. kunmingensis (ZHKUCC 22-0242) (Liu et al. 2024). Based on morphological characteristics and phylogenetic analyses, we report our isolation as the first record of N. kunmingensis from decaying wood of Castanopsis mekongensis in China.
Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, tef1-α, and rpb2 sequence data, representing Nigrograna (Nigrogranaceae). Related sequences are obtained following de Lu et al. (2022b) and Boonmee et al. (2021). Forty-six strains are included in the combined analyses, which comprise 4194 characters for SSU, LSU, ITS, tef1-α, and rpb2 alignment. Seriascoma didymospora (MFLUCC 11-0194, MFLUCC 11-0179) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -17623.075885 is presented. The matrix had 1076 distinct alignment patterns, with 31.53% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.248028, C = 0.248350, G = 0.266441, T = 0.237180; substitution rates AC = 1.506227, AG = 4.589175, AT = 1.553542, CG = 1.033267, CT = 10.958332, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.
Figure 2. Nigrograna kunmingensis (HKAS 122729). a Material examined. b, c Appearance of ascomata on the host substrate. d, e Section of an ascoma. f Peridium. g Pseudoparaphyses. h–o Asci. p–v Ascospores. w Germinated ascospore. x, y Culture characters on PDA (x = from above, y = from below). Scale bars: d, e = 200 μm, f = 50 μm, g = 25 μm, h–o = 30 μm, p–w = 10 μm, x, y = 30 mm.
References
Recent Genus
AmphisphaeriaParaeutypella
Halorosellinia
Recent Species
Torula chromolaenaePericonia delonicis
Neopestalotiopsis theobromicola