Pleosporales » Melanommataceae » Byssosphaeria

Byssosphaeria musae

Byssosphaeria musae Phook. & K.D. Hyde, in Liu et al., Fungal Diversity: 10.1007/s13225-015-0324-y, [119] (2015)

Index Fungorum number: IF550932   Facesoffungi number: FoF00436

 

Saprobic on leaf sheath of Musa sp. Sexual morph: Ascomata 430–540μm high, 450–630μm diam, gregarious, scattered, superficial on subiculum, visible as dark spots on host, orange to yellow around pore, uni-loculate, globose to subglobose, setose, apexrounded, ostiolecentral, withpore-like opening. Peridium 35–80μm wide, thick-walled, of equal thickness, composed of several layers of dark brown to black cells, arranged in textura angularis to textura prismatica. Hamathecium 0.5–1.7μm wide, composed of dense, trabeculate, distinctly septate, anastomosing, pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci (120–)125–135(–145)×(11.5–)12–14(–17) μm (x=132.8×13.7μm, n=25), 8- spored, bitunicate, fissitunicate, clavate, long pedicellate (40–50μm long) with knob-like pedicel, apically rounded, with well-developed ocular chamber. Ascospores (27–)30–33(–36)×(4–)5–6 μm (x=32.3×5.9 μm,n=30), overlapping1–2-se-riate, fusiform, with acute ends, hyaline to pale brown when young, becoming light brown at maturity, 1(–3)-septate, not constricted at the septa, slightly curved, smooth-walled, bearing delicate hyaline appendages over ends with wing-like appendages near the central septum. Asexual morph: Undetermined.

 

Culture characters: Colonies on PDA fast growing, 80–90 mm diam. after 2 weeks at 25–30 °C, white to cream or pale yellowish, intermixed with yellowish to orangish hyphae; reverse cream to white yellowish at the margin, yellowish-brown at the centre, medium dense to dense, irregular, flattened to slightly raised, dull with entire edge, fluffy to feathery, effuse, zonate.

 

Material examined: THAILAND: Chiang Rai, Muang District, Khun Korn Waterfall, on leaf sheath of Musa sp. (Musaceae), 5 September 2010, R. Phookamsak RP0062 (MFLU 11–0182, holotype); ex-type living culture, MFLUCC 11–0146. GenBank ITS: KP744435; LSU: KP744477; SSU: KP753947.

 

Notes: Byssosphaeria musae is similar to B. salebrosa (Cooke & Peck) M.E. Barr and B. schiedermayeriana (Fuckel) M.E. Barr (1984; Mugambi and Huhndorf 2009), with ascomata, asci and ascospores having a similar size range, but being smallest in B. musae which has smaller ascomata, asci and ascospores than these species. Byssosphaeria musae differs from B.schiedermayeriana in ascospore appendages, which are present in B. musae, but lacking in B. schiedermayeriana. Phylogenetic analyses of combined genes showed that Byssosphaeria musae forms a separate robust clade (77 % MP and 67 % ML) close to B. schiedermayeriana and B. salebrosa.