Pleosporales » Massarinaceae » Vaginatispora

Vaginatispora palmae

Vaginatispora palmae S.N. Zhang, J.K. Liu & K.D. Hyde, in Hyde et al., Fungal Diversity: 10.1007/s13225-019-00429-2, [43] (2019)

Index Fungorum number: IF556316         Facesoffungi number: FoF05089

 

Sexual  morph: Ascomata in vertical section 250–340 µm high, 215–385 µm diam. (x̅ = 310 × 326 µm, n = 10), dark brown to black, scattered, semi-immersed, erumpent, sub-globose to elongated, base flatted, coriaceous to carbonaceous. Ostiole crest-like, variable in shape, central papillate. Peridium 15–38 µm wide, wider at the apex and thinner at the base, composed of several pale brown to brown cells of textura angularis, cells towards the inside lighter, at the outside darker, somewhat flattened, fusing and with the host tissues. Pseudoparaphyses 1–2.5 µm wide, hypha-like, numerous, septate, rarely branched and anastomosed, tapering towards the apex. Asci 89–115 × 12–20 µm (x̅ = 100.5 × 16.0 µm, n = 20), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with a short bulbous pedicel, rounded at the apex, with an ocular chamber. Ascospores 23–45 × 6–9 µm (x̅ = 35.3 × 7.5 µm, n = 30), hyaline, uniseriate or over-lapping to biseriate, 1-septate, occasionally producing pseudosepta, slightly constricted at the central septum, cell above central septum swollen, guttulate, smooth-walled, surrounded by a narrow mucilaginous sheath and drawn out towards each end to form tapering appendages, 6–8 lm long. Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on PDA within 24 h. Colonies growing on PDA reaching 2 cm diam. after 21 days at 25 , the off-white hyphae in first week, then becoming grayish blue and dark bluish, composed of brown to dark brown, septate, smooth or verrucose hyphae.

 

Habitat: Saprobicon on immersed rachis of Nypa fruticans Wurmb (Arecaceae).

 

Known distribution: Thailand.

 

Material examined: THAILAND, Ranong Province, on immersed rachis of Nypa fruticans Wurmb (Arecaceae), 3 December 2016, S.N. Zhang, SNT92 (MFLU 18–1586, holotype), ex-type living culture (MFLUCC 18–1526); ibid. (HKAS 102207, isotype).

 

GenBank numbers: ITS: MK085055, LSU: MK085059, SSU: MK085057, TEF1-a: MK087657.

 

Notes: Most species of Vaginatispora are found in tropical regions and commonly occur in freshwater and marine environments, but only one species V. nypae Jayasiri, E.B.G. Jones & K.D. Hyde (Jayasiri et al. 2019) has recently been recorded from a Nypa fruticans. Almost all Vaginatispora species have 1-septate ascospores with terminal appendages or sheaths (Hashimoto et al. 2018). Detailed characters of ascospores are useful for distinguishing taxa at the species level, and molecular sequence data is key for identification of taxa in this group. The multi-gene analysis includes all species in this genus and the new isolate Vaginatispora palmae clustered with V. amygdali. Vaginatispora palmae differs from V. amygdali because the latter species has a lateral pad-like structure within the sheath and an internal chamber at both ends of the ascospores, while V. palmae lacks those structures. They also differ in peridium width (15–38 µm vs. 37.5–62.5 µm), ascus (mean: 100.5 × 16 µm vs. 115 ×9 18.5 µm) and ascospores size (mean: 35.3 × 7.5 µm vs. 30.6 × 8.8 µm). In addition, polymorphic nucleotide comparison shows that these two strains differ in eight positions in the ITS1 and ITS2 regions including two gaps, and differ in eight positions in the TEF1-a region. The difference in molecular sequence data also distinguish V. palmae from V. amygdali, therefore, we introduce a new species V. palmae.