Pleosporales » Massarinaceae » Vaginatispora

Vaginatispora nypae

Vaginatispora nypae Jayasiri Jayasiri, E.B.G. Jones & K.D. Hyde, in Jayasiri, Hyde, Jones, McKenzie, Jeewon, Phillips, Bhat, Wanasinghe, Liu, Lu, Kang, Xu & Karunarathna, Mycosphere 10(1): 84 (2019)

Index Fungorum number: IF555558    Facesoffungi number: FoF05264


Saprobic on brackish water palm Nypa fruticans. Sexual morph: Ascomata 305–360 µm high × 280–405 µm diam. ( x = 344 × 365 µm; n = 30), scattered to gregarious, immersed, coriaceous, dark brown to black, surrounded by large, blackened parenchymatous cells, ostiolate. Ostiole slitlike, central, with a reduced crest and a pore-like opening, plugged by gelatinous tissue, made up of lightly pigmented, periphyes. Peridium 22–30 µm wide, circular, symmetric, dark brown to black 85 layers, somewhat flattened cells of textura angularis, fusing and indistinguishable from the host tissues, inner stratum comprising hyaline cell layers of textura angularis. Hamathecium 1.0–1.5 µm wide ( x = 1.3 µm; n = 30), comprising numerous, filamentous, branched septate, pseudoparaphyses. Asci 75–85 × 11–13 µm ( x = 82 × 11.9 µm; n = 20), 8-spored, bitunicate, fissitunicate, cylindrical-clavate, short-pedicellate, apex rounded with a minute ocular chamber. Ascospores 22–26 × 10–13 µm ( x = 24 × 12 µm; n = 30), uniseriate to bi-seriate, overlapping, hyaline, fusiform with narrow, acute ends, 8-septate, constricted at the septa, smooth-walled, guttulate, with a prominent mucilaginous sheath. Asexual morph: Undetermined.


Culture characters: Conidia germinated on MEA within 24 hr. Colonies on MEA reaching 3–4 mm diam. after 4 weeks at 18°C, colonies irregular, medium dense, brown to grey in top view with pale brown edge. Lower surface dark brown with radially arrange margin.


Material examined: THAILAND, Krabi Province, Mueang Krabi District, on fallen fruit pericarp of Nypa fruticans (Arecaceae), 31 Aug 2017, S.C. Jayasiri C 350 (MFLU 18–2156, holotype), ex-type living culture MFLUCC 18–1543, KUMCC 18–0302.


GenBank numbers: SSU: MK347872, ITS: MK347765, LSU: MK347982, tef1: MK360091, rpb2: MK434877


Notes: Vaginatispora nypae forms a sister clade to V. appendiculata (MFLUCC 16–0314) with good statistical support (78 % MLBS/0.95 BYPP) and shares similar morphological characters. Both species have cylindrical to clavate asci and hyaline, narrow fusiform ascospores with sheaths. However, V. nypae lacks a slit-like ostiole, appendages and ascospores with a wing like sheath seen in V. appendiculata (Wanasinghe et al. 2016). Vaginatispora nypae was collected from estuarine zone in Thailand, while V. appendiculata is known from a fresh water environment in Thailand. A comparison of the ITS nucleotides of these two strains reveals 58 (10.7%) nucleotide differences, which indicates that they are distinct taxa (Jeewon & Hyde 2016).


Figure X. Vaginatispora nypae (MFLU 18–2156, holotype). a Host fruit. b Ascoma on substrate. c, d Section through ascoma. e Peridium. f Pseudoparaphyses. g Ascus. h–i Ascospore. j Ascospore stained with Indian ink. k Germinated ascospore. Scale bars: a = 1 cm, b = 500 µm, c, d = 100 µm, e = 30 µm, f, h–k = 10 µm, g = 20 µm.


Reference: Hyde KD, de Silva NI, Jeewon R, Bhat DJ, et al. 2020 – AJOM new records and collections of fungi: 1-100. Asian Journal of Mycology 3(1), 22–294.




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