Paramassaria samaneae
Paramassaria samaneae Samarak & K.D. Hyde, in Hyde et al., Fungal Diversity: 10.1007/s13225-019-00429-2, [49] (2019)
Index Fungorum number: IF555522 Facesoffungi number: FoF05214
Sexual morph: Ascomata 520–580 μm high (x̄ = 540 μm, n = 15), 410–560 μm diam. (x̄ = 490 μm, n = 15), immersed or semi-immersed in the yellow stained host, solitary, scattered, coriaceous, globose to subglobose, brown to dark brown. Ostiole central, flattened on the top, surrounded by blackish stromatic zone on the host, ostiolar canal with hyaline periphyses. Peridium 20–43 μm wide (x̄ = 33.5 μm, n = 25), inner cell layer hyaline, thin and outer cell layer comprising dark brown textura angularis cells. Hamathecium comprising 4.2–10 μm wide (x̄ = 6.6 μm, n = 25), hyaline, cylindrical to filiform, septate, branched pseudoparaphyses. Asci 190–215 9 45.5–56 μm (x̄ = 212.5 × 50.5 μm, n = 20), 8-spored, bitunicate, oblong to cylindrical, short pedicellate. Ascospores 52.5–71.5 × 13.5–18.5 μm (x̄ = 61.5 × 16 μm, n = 50), overlapping 1–2-seriate, brown, ellipsoid to broadly fusiform, 3-septate, constricted at the septa, with 4 guttlues, surrounded by hyaline gelatinous sheath observed clearly when mounted in Indian ink. Asexual morph: Undetermined.
Culture characteristics:
Habitat:
Known distribution:
Material examined: Thailand, Chiang Rai Province, Muang District, Mae Fah Luang University, on a dead branch of Samanea saman (Fabaceae), 2 August 2017, MC. Samarakoon, SAMC002 (MFLU 17-1551, holotype; HKAS 102338, isotype).
GenBank Accession No: LSU: MK108190, MK108191, SSU: MK108187, MK108188, TEF1: MK105747, MK105747.
Notes: Paramassaria samaneae was collected on a dead branch of Samanea saman from Thailand. This taxon is similar to Massaria in its ascomata, asci and ascospore characters. The wide ostiole and absence of an ocular chamber in the asci distinguishes our taxon from Massaria.
However, the combined phylogenetic analyses separate our taxon as a basal clade to Massaria. The closest BLAST search with the LSU and TEF1-a sequences of MFLU 17-1551 were GQ221892 (with 93% identity) Rhytidhysteron opuntiae and FJ161103 (with 93% identity) Gloniopsis praelonga belonging to Hysteriaceae. Only ITS, LSU and SSU sequences are available for Massarioramusculicola and resulted in inconsistent topologies with the absence sequence data. In addition, we observed that our strains have probabilities to cluster in Aigialacaeae as a basal clade. However, Massarioramusculicola differs from Massaria and Paramassaria by having a peridium with two strata with dark brown to hyaline cells of textura angularis, rarely branched, septate and trabeculate pseudoparaphyses and 3-septate hyaline ascospores. Including parallel morphology to Massaria, we prefer to place our strains into a new genus in Massariaceae. Voglmayr and Jaklitsch (2011) suggested that the ascospore and pseudothecia characters are important for the identification of phylogenetically distinct Massaria species. The strong host-specificity among Massaria species makes it complex for accurate identification (Voglmayr and Jaklitsch 2011). It might also not be a surprise if future discoveries of more species within Massariaceae converge the clade and there is a need to aggregate them into one genus, but it is unwise to do this at this stage.
Figure X. Paramassaria samaneae (MFLU 17–1551, holotype). a, b Ascomata on the substrate. c Horizontal section of ascoma. d Vertical section of ascoma. e Ostiole section. f Peridium (in water). g Pseudoparaphyses. h–j Asci. k–s Ascospores (s-in Indian ink). Scale bars: a = 1000 μm, b–d = 200 μm, e = 100 μm, h–j = 50 μm, f, g, k–s = 20 μm.
Reference: Hyde KD, Tennakoon DS, Jeewon R, Bhat DJ, et al (2019) Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96(1): 1–242.
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