Pleosporales » Lophiotremataceae » Crassimassarina

Crassimassarina baoshanensis

Crassimassarina baoshanensis G.C. Ren & K.D. Hyde, in Ren, Jayasiri, Tibpromma, Farias, Chethana, Faraj, Wanasinghe, Xu, Hyde & Gui, Mycosphere 15(1): 1026 (2024)

Index Fungorum number: IF 901353; Facesoffungi Number: FoF13884

 

Saprobic on dead woody twigs of Castanopsis orthacantha. Sexual morph: Ascomata 270–400 μm high × 250–300 μm diam., (x̅ = 350 × 285 μm, n = 5), immersed, solitary or scattered, coriaceous, globose to subglobose, black. Ostioles central, 120–140 μm high × 110–160 μm diam., (x̅ = 135 × 136 μm, n = 5), globose in section. Peridium 12–22 μm wide, 2–4-layered, comprising brown cells of textura angularis. Hamathecium 1–2 μm wide, comprising cylindrical, branched, septate, anastomosed pseudoparaphyses embedded in a hyaline gelatinous matrix. Asci 130–180 × 24–29 μm (x̅ = 155 × 27 μm, n = 20), 8-spored, bitunicate, clavate, straight or curved, apically rounded, short pedicellate, with a minute ocular chamber. Ascospores 37–45 × 10–13 μm (x̅ = 40 × 11.5 μm, n = 30), overlapping biseriate, broadly fusiform with rounded ends, straight, initially hyaline, becoming brown at maturity, 1(–3)-septate, strongly constricted at the septum, guttulate in each cell, thick and smooth-walled, with a gelatinous sheath. Asexual morph: Undetermined.

Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the apical cell of ascospore. Colonies on PDA, reaching 40–50 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, dense, flat, entire edge, gray white at the center, atrovirens at the margin; reverse, atrovirens.

 

Material examined: China, Yunnan Province, Baoshan, on dead woody twigs of Castanopsis orthacantha (Fagaceae), 12 July 2020, G.C. Ren, BS07 (HKAS 122702, holotype), ex-type living culture KUMCC 21-0499; ibid., BS08 (HKAS 122703, isotype), ex-isotype living culture KUMCC 21-0500.

 

GenBank numbers: KUMCC 21-0499: SSU: OQ168212, LSU: OQ170858, ITS: OQ158936, tef1-α: OR613433; KUMCC 21-0500: SSU: OQ168213, LSU: OQ170859, ITS: OQ158937, tef1-α: OR613434.

 

Notes: Crassimassarina baoshanensis is introduced as a new species based on its distinct morphology and the phylogeny of the combined SSU, LSU, ITS, and tef1-α dataset. Our species formed a sister clade to C. macrospora with 100% ML bootstrap support and 1.00 BYPP value. Our species is similar to C. macrospora in having globose to subglobose ascomata, papillate ostiolar, cylindrical asci and broadly fusiform ascospores with a gelatinous sheath. However, C. baoshanensis differs from C. macrospora in having coriaceous, globose ostioles, 12–22 μm wide, a 2–4-layered peridium, clavate asci and brown ascospores, while C. macrospora has carbonaceous ostioles, 25–60 μm wide, composed of 6–8 layers of peridium, cylindrical asci and hyaline ascospores (Hashimoto et al. 2017). Sequence comparison for the ITS region between Crassimassarina baoshanensis (KUMCC 21-0499) and C. macrospora (KT 1764) showed a 1.5% (7/484 bp, without gaps) base pair difference in ITS region, 1% (9/981 bp, without gaps) base pair difference in tef1-α region, 0.6% (5/850 bp, without gaps) base pair difference in LSU region.

 

 

Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, and tef1-α sequence data, representing Lophiotremataceae. Related sequences are obtained following de Silva et al. (2018) and Phookamsak et al. (2019). Thirty-one strains are included in the combined analyses, which comprise 3639 characters for SSU, LSU, ITS, and tef1-α alignment. Lophiostoma arundinis (CBS 621.86) and L. crenatum (CBS 629.87) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -11856.946004 is presented. The matrix had 630 distinct alignment patterns, with 9.85% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.244862, C = 0.255944, G = 0.269198, T = 0.229995; substitution rates AC = 1.462154, AG = 2.709696, AT = 1.642078, CG = 1.234650, CT = 9.229432, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.

 

 

Figure 2. Crassimassarina baoshanensis (HKAS 122702, holotype). a Material examined. b Appearance of ascomata on the host substrate. c Section of an ascoma. d Peridium. e Pseudoparaphyses. f–k Asci. l–o Ascospores. p Ascospore stained in Indian ink to show the mucilaginous sheath. q Germinated ascospore. r, s Culture characters on PDA (r = from above, s = from below). Scale bars: c = 200 μm, d, f–k = 50 μm, e = 20 μm, l–q = 30 μm, r, s = 20 mm.

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

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