Pleosporales » Lophiostomataceae » Neovaginatispora

Neovaginatispora fuckelii

Neovaginatispora fuckelii (Sacc.) A. Hashim., K. Hiray. & Kaz. Tanaka, in Hashimoto, Hirayama, Takahashi, Matsumura, Okada, Chen, Huang, Kakishima, Ono & Tanaka, Stud. Mycol. 90: 188 (2018)

Index Fungorum number: IF823148         Facesoffungi number: FoF06527

 

Sexual morph: Ascomata 250–580 × 190–230 × 210–250 μm (x̄ = 400 × 220 × 235 μm, n = 5), perithecial, solitary and scattered, immersed to semi-immersed, unilocular, subglobose to ellipsoid, black, ostiolate. Ostiole rounded or crest-like, periphysate. Peridium 10–40 μm wide, outer layer composed of brown to dark brown, thick-walled cells of textura angularis; inner layer composed of hyaline to lightly pigmented cells of textura prismatica. Hamathecium comprising 1.5–2.5 μm wide, numerous, filiform, septate, branched, anastomosed pseudoparaphyses, embedded in a gelatinous matrix. Asci 45–75 × 6–10 μm (x̄ = 60 × 8 μm, n = 40), 8-spored, bitunicate, fissitunicate, cylindrical to clavate, short-pedicellate, rounded at the apex, with a distinct ocular chamber. Ascospores 10–15 × 4–6 μm (x̄ = 14 × 5 μm, n = 50), 1–2-seriate, partially overlapping, hyaline, broadly fusiform with obtuse ends, 1-septate at median, constricted, slightly inflated of the cell nearby septum, straight or slightly curved, smooth-walled, guttulate, with cap-shaped, globose appendages at each end.

 

Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on PDA within one week at 23 °C. Colony 1.5 cm diam. After 30 days. Mycelium superficial to semi-immersed, filamentous, branched, septate, hyaline to brown. Colonies on PDA, initially, pale brown, becoming dark brown, rough surface and raised elevation with concentric zonations, uneven margins; reverse pale brown to dark brown at the zonations.

 

Material examined: CHINA, Yunnan Province, Kunming, Songhuaba Lake, on decaying wood of Prunus sp. (Rosaceae), 3 September 2017, S.K. Huang, SHB04 (KUNHKAS99593), living culture (KUMCC 17-0319).

 

 

Habitat: -

 

Known hosts: This species has been recorded from various dead herbaceous plants and leaves of Mangifera in Europe, Japan and China (Wang and Lin 2004; Thambugala et al. 2015; Hyde et al. 2016, 2019a; Tennakoon et al. 2018b).

 

Known distribution: -

 

Material examined: CHINA, Yunnan Province, Kunming City, Kunming Institute of Botany, on dead hanging branches of Lonicera maackii, 20 April 2017, R. Phookamsak, KIB031 (KUN-HKAS 102233, holotype), ex-type living culture, KUMCC 18-0159 (KIB031A), KUMCC 18-0160 (KIB031B).

 

GenBank Accession No: ITS: MN473470, LSU: MN473513, RPB2: MN482130.

 

Notes: Hashimoto et al. (2018) found that Vaginatispora fuckelii is phylogenetically distinct from other species of Vaginatispora based on their multi-gene phylogenetic analysis. They proposed a monotypic genus Neovaginatispora typified by N. fuckelii (Hashimoto et al. 2018). In this study, our new strain clusters with other N. fuckelii strains in the phylogenetic analysis. The present collection shares similar morphological features with previously reported collections, but the ascomata and ascospore dimensions are slightly different (Thambugala et al. 2015; Hyde et al. 2016; Tennakoon et al. 2018b). Some morphological characters may be slightly different on different host species and locality (Thambugala et al. 2015; Hyde et al. 2016; Tennakoon et al. 2018b). In Tennakoon et al. (2018b), N. fuckelii was found on Mangifera indica and its ascospores have a thin mucilaginous sheath. This is the first record of Neovaginatispora species on dead branches of Prunus species.

Figure X. Neovaginatispora fuckelii (KUN-HKAS 99593). a Material. b Appearance of ascomata on the host. c Crest-like ostiole of ascoma. d Section through ascoma. e, f Peridium. g–i Asci with pseudoparaphyses. j–m Ascospores. n Germinating ascospore. Notes: l, m stained in black ink reagent. Scale bars: c = 500 μm, d = 200 μm, e = 100 μm, f–i, n = 50 μm, j–m = 10 μm

Reference:

: Hyde KD, Dong Y, Phookamsak R, Jeewon R et al. (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity (2020) 100:5–277

 

Recent Genus

Boeremia
Septofusispora
Neolophiotrema

Recent Species

Boeremia maritima
Boeremia albae
Septofusispora thailandic