Pleosporales » Lophiostomataceae » Lophiotrema

Lophiotrema mucilaginosis

Lophiotrema mucilaginosis M. Raza & L. Cai, in Phookamsak et al., Fungal Diversity: 10.1007/s13225-019-00421-w, [32] (2019)

Index Fungorum number: IF555333         Facesoffungi number: FoF04941

 

Sexual morph: Ascomata 220–340 µm high, 240–400 µm diam., black, scattered, solitary, semi-immersed to erumpent through host surface, conical to mammiform, with flattened base, uni-loculate, glabrous, ostiolate, papillate. Ostioles 50–90 × 25–60 µm, apically with crest-like papilla, filled with periphyses, carbonaceous, with beak-like opening. Peridium 55–80 lm wide, outer layer thick, composed of dark, coriaceous, pseudoparenchymatous cells of textura epidermoidea, inner layer comprising light pigmented to hyaline cells of textura angularis. Hamathecium composed of branched, 1.5–2 µm wide, filamentous, indistinct septate, anastomosed pseudoparaphyses, embedded in a hyaline, gelatinous matrix. Asci (83.5–) 102–144(–210) × (10.5–)13–15(–21.5) µm (x̄ = 127.5 × 14.5 µm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate with furcate to truncate pedicel, apically rounded, with well-developed ocular chamber. Ascospores (31.5–) 34–45.5(–48) × (5.5–) 6–11(–12.5) µm (x̄ = 39.1 × 8.6 µm, n = 40), overlapping 2-seriate, hyaline, subfusoid to fusiform, with rounded or obtuse ends, 1(–3)-septate, smooth-walled, guttulate when young, with an entire mucilaginous sheath (9–20.5 µm wide at sides). Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA reaching 2.5–3 mm diam. after 1 week at 25 ± 2 °C, circular, convex or dome-shaped, rough with entire edge, mucoid, smooth at the margin; from above, green earth at the fruiting zone, grey at the productive zone and light grey at ageing zone, dome, shining black mucoid colony; from below, light grey at the fruiting zone, light green to blackish at the productive zone, dark grey at the ageing zone; cracking and not producing pigmentation in PDA agar medium.

 

Habitat: -

 

Known hosts: -

 

Known distribution: -

 

Material examined: CHINA, Yunnan Province, Baoshan City, Longling County, on wood litter, October 2015, M. Raza, BAP 119 (HMAS 255437, holotype), ex-type living culture, LC12112.

 

GenBank Accession No: ITS: MH822889, LSU: MH822890, SSU: MH822891, RPB2: MH822892, TEF1-a: MH822893.

 

Notes: Multi-loci phylogenetic analyses based on a concatenated ITS, LSU, SSU, TEF1-a and RPB2 sequence dataset show that Lophiotrema mucilaginosis forms a wellsupported lineage (100% ML and 1.00 BYPP) sister to L. hydei J.F. Zhang et al. and clusters with L. neohysterioides M.E. Barr. Lophiotrema mucilaginosis and L. hydei were collected on wood litter and herbaceous plant from Yunnan and Guizhou Provinces in China respectively. Lophiotrema mucilaginosis can be distinguished

from L. hydei in having larger asci (78–89(–99) ×  6.9–8.8 µm, L. hydei) and larger ascospores (23–28 ×  3–4 µm, L. hydei) (Zhang et al. 2018). In addition, L. mucilaginosis has conical to mammiform ascomata, with flattened base and 1(–3)-septate ascospores. Whereas, L. hydei has globose to subglobose ascomata and inconspicuously 0–1-septate ascospores. Lophiotrema mucilaginosis differs from L. neohysterioides by its larger asci (70–96 ×  7–10 µm, L. neohysterioides) and larger ascospores (17–25 ×  3–5 µm, L. neohysterioides) and presence of mucilaginous sheath surrounding the ascospores, a feature not observed in L. neohysterioides (Tanaka and Harada 2003a).

Figure X. Lophiotrema mucilaginosis (HMAS 255437, holotype).a Blackish ascomata on dead wood. b Vertical section of ascoma. c Peridial structure. d Pseudoparaphyses. e, f Immature asci. g, h Mature asci. i Apical ring stained with cotton blue. j Immature ascospores. k–o Mature ascospores. p Ascospores with mucilaginous sheath. q Germination of ascospore. r, s Culture characteristics on PDA (r = from above, s = from below). Scale bars b = 100 µm, c = 50 µm, d, p, q = 20 µm, e–i = 10 µm, o = 5, j–n = 2 µm.

Reference:

Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, et al. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity (2019) 95:1–273

 

 

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