Pleosporales » Leptosphaeriaceae » Plenodomus

Plenodomus collinsoniae

Plenodomus collinsoniae (Dearn. & House) Gruyter, Aveskamp & Verkley, Stud. Mycol. 75: 21 (2012)

Facesoffungi number: FoF07379

Saprobic on dead branch of an unidentified host. Sexual morph: Ascomata 245–260 μm high, 360–390 μm diameter (x̅= 255 × 380 μm, n = 20), solitary, scattered, appearing as small, raised black dots on the host surface, superficial or semi immersed, globose to sub globose, smooth, with a flattened top and base and, brownish black, ostiolate, papillate. Papilla central circular. Peridium 45–55 μm (x̅= 52 μm, n = 10) at sides, 20–30 μm (x̅= 28 μm, n = 10) at the base, slightly thin at the base, composed of three layers of scleroplectenchymatous cells, inner layer comprising 2–3 cell layers of flattened, light brown cells, arranged in a textura angularis, middle layer comprising several layers of subhyaline cells arranged in a textura globulosa, outer layer composed of heavily pigmented, thick-walled, dark brown cells, textura angularis. Hamathecium comprising 1.5–2.5 μm (x̅ = 2.2 μm, n = 10) wide, septate, cellular pseudoparaphyses, branching between the asci, embedded in a gelatinous matrix. Asci 105–210 × 15–25 μm (x̅= 170 × 23 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical, short pedicel, apically rounded, with a distinct ocular chamber. Ascospores 40–70 × 6–10 μm (x̅= 65 × 9 μm, n = 20), overlapping uni- or bi-seriate, olivaceous to yellowish, cylindrical to subfusoid, with obtuse ends, 6–7 euseptate, not constricted at each septum, cell above central septum slightly wider, guttulate, thick and smooth-walled, with mucilaginous globoid-shaped apical and basal appendages. Asexual morph: Undetermined.

 

Known distribution: China, Japan (De Gruyter et al. 2013, MarinFelix et al. 2017b), China (this study).

 

Known hosts: Collinsonia canadensis, Vitis coignetiae, Unidentified dead wood (De Gruyter et al. 2013, Marin-Felix et al. 2017b).

 

Material examined: China, Yunnan Province. Kunming, dead branch of an unidentified host, 15 December 2018, V. Thyagaraja, DXH 015 (MFLU 19–2279, new sexual morph record). GenBank accessions – LSU MN982862, SSU MN982863, ITS MN982858.

Figure X. Plenodomus collinsoniae (MFLU 19–2279, new sexual morph record). a–b Ascomata on host. c Vertical section of ascoma. d Section of peridium. e–f Asci. g Pseudoparaphyses. h–j Ascospores. Scale bars: b–c = 50 μm, d–j = 20 μm, k– o = 5 μm.

 

Notes: Plenodomus collinsoniae is only known from its phoma-like asexual morph (De Gruyter et al. 2013). In our phylogram, new strain (MFLU 19–2279) grouped together with P. collinsoniae (CBS 120227) with high statistical support (99 % ML, 1.00 PP) (Fig. 49). Furthermore, DNA sequences of SSU, LSU and ITS of our strain (MFLU 19–2279) are almost similar to P. collinsoniae (CBS 120227). Therefore, it is confirmed that our new strain is the asexual morph of P. collinsoniae. Plenodomus collinsoniae showed close morphological and phylogenetic similarities to the type of the recently introduced P. sinensis. However, they are morphologically different by their asci (105–210 × 15–25 vs. 80–100 × 10–12 μm) and ascospore sizes (40–70 × 6–10 vs. 27–40 × 3.8–4.4 μm). Additionally, 9 base pairs of the ITS sequence of Plenodomus collinsoniae and P. sinensis are different out of 536 bp without gaps (1.7 %).

 

Reference:        Hyde KD, de Silva NI, Jeewon R, Bhat DJ, et al. 2020 – AJOM new records and collections of fungi: 1-100. Asian Journal of Mycology 3(1), 22–294.

 

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