Pleosporales » Leptosphaeriaceae » Plenodomus

Plenodomus artemisiae

Plenodomus artemisiae A. Karunarathna, Phookamsak & K.D. Hyde, in Phookamsak et al., Fungal Diversity Notes: 10.1007/s13225-019-00421-w, [23] (2019)

Index Fungorum number: IF556118,        Facesoffungi number: FoF 05696

 

Sexual morph: Ascomata 140–280 μm high, 300–450 μm diam., black, shiny on the host surface, solitary to aggregated, immersed at the base, becoming superficial, uniloculate, subglobose to irregular in shape, with truncate base, glabrous, ostiolate, papillate. Ostioles central, dark. brown, beak-like papilla, ostiolar canal filled with periphyses. Peridium 10–85 μm wide, thick-walled of unequal

thickness, thickened at the based, slightly thin at the apex, composed of several cell layers of dark brown scleroplectenchymatous cells, arranged in a textura angularis to textura globulosa. Hamathecium composed of hyaline, filamentous, 2–4 μm wide, distinctly septate pseudoparaphyses,

anastomosing, embedded in a hyaline gelatinous matrix. Asci (64–)70–90(–100) × (9.5–)10–13 μm

(x̄ = 82.7 × 11.2 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindrical, short, pedicellate, apically rounded with a distinct ocular chamber. Ascospores (28–)30–40 × (4.5–)5–6 µm (x̄= 34.4 × 5.5 µm, n = 30), overlapping 2–3-seriate, pale brown, fusiform, 5-septate, slightly constricted at the septa, enlarge at the third cell from above, lacking a mucilaginous sheath and appendages. Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA reaching 30–33 mm diam. after 4 weeks. Colony dense, circular, low convex, surface smooth, with edge entire, floccose; from above white; from below, yellowish-grey at the edge, with white to cream margin, dark yellowish at the centre, slightly radiating outwards colony; not produced pigmentation on agar medium.

 

Material examined: CHINA, Yunnan Province, Kunming City, Kunming Institute of Botany, on dead branches and stems of Artemisia sp., 20 December 2015, R. Phookamsak, AS003 (KUN-HKAS 102226, holotype). extype living culture, KUMCC 18-0151.

 

GenBank Accession No: ITS: MK387920, LSU: MK387958, SSU: MK387928, TEF1-a: MK435600,

RPB2: MK435607 (KUMCC 18-0151).

 

Notes: Plenodomus artemisiae forms a distinct lineage and is sister to P. salviae Thambug. et al. (MFLUCC 13-0219) with high support (99% ML and 0.99 BYPP). Plenodomus artemisiae can be distinguished from P. salviae in having shorter and broader, pale brown, fusiform ascospores, enlarged at the third cell from above. Plenodomus salviae has longer and thinner, yellowish brown, cylindric-fusiform ascospores (30–48 × 3.1–4.3 lm), and without the enlarged cell (Ariyawansa et al. 2015b). Plenodomus artemisiae is also similar to Leptosphaeria artemisiae (Fuckel) Auersw. in having

5-septate ascospores, with the enlarged third cell and occurring on Artemisia. However, L. artemisiae has larger ascomata and narrower ascospores (32–37 × 8.5–10; Shoemaker 1984). Furthermore, P. artemisiae has pale brown, fusiform ascospores, whereas, L. artemisiae has light reddish brown, broadly elliptical ascospores (Shoemaker 1984). Phylogenetic affinity of L. artemisiae could not be resolved due to lack of molecular data.

 

Figure X. Plenodomus artemisiae (KUN-HKAS 102226, holotype). a Ascomata on host. b, c Vertical section of ascomata. d Ostiole. e Section through peridium. f Pseudoparaphyses. g–i Asci. j–m Ascospores. n Ascospore germination. o, p Culture characteristics (o = from above, p = from below). Scale bars a = 500 µm, b, c = 100 µm, d, e = 50 µm, f–i, n = 20 µm, j–m = 10 µm.

 

Reference: Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, et al. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity (2019) 95:1–273

 

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