Pleosporales » Lentitheciaceae » Keissleriella

Keissleriella caraganae

Keissleriella caraganae Chaiwan, Phookamsak, Wanas. & K.D. Hyde, Fungal Diversity 95: 16 (2019).

Index Fungorum number: IF555523         Facesoffungi number: FoF04965


Saprobic on Caragana arborescens (Fabaceae). Sexual morph: Ascomata 140–175 μm high, 170–235 μm diam., scattered, solitary or in groups, semi-immersed, visible as raised, black dots on host surface, globose to subglobose, glabrous, ostiolate at centre, with minute papilla, filled with short, brown, aseptate periphyses. Peridium 15–25 μm wide, thin-walled, of equal thickness, composed of several layers of small, flattened, brown to dark brown pseudoparenchymatous cells, arranged in a textura angularis to textura prismatica, intermixed with the host cells. Hamathecium composed of dense, 2–3 μm wide, broad filamentous, distinctly septate, anastomosed pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci 39–75 × 10–12 μm (x̄ = 60.1 × 11.1 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate, apically rounded, with well-developed ocular chamber. Ascospores 14–20 × 3–7 μm (x̄ = 16.9 × 5.1 μm, n = 20), overlapping 1–2-seriate, pale yellowish, fusiform to ellipsoidal, with rounded ends, (1–)3(–4)-septate, slightly constricted at the central septum, smooth-walled, with small guttules, surrunded by a distinct mucilaginous sheath. Asexual morph: Undetermined.


Culture characteristics: Colonies on PDA reaching 22–29 mm diam. after 1 week at 20–25 °C, colony from above, white to cream at the margin, greenish grey in the centre; from below, white to cream at the margin, greenish grey in the centre; medium dense, circular, slightly raised, surface smooth, with edge entire, floccose to velvety, not producing pigmentation in agar.


Habitat: on dead hanging branch of Caragana arborescens Lam. (Fabaceae)


Known distribution: China


Material examined: CHINA, Yunnan Province, Kunming Institute of Botany, on dead hanging branch of Caragana arborescens Lam. (Fabaceae), 2 November 2017, R. Phookamsak, KIB018 (KUN-HKAS 102236, holotype), ex-type living culture, KUMCC 18-0163 = MFLUCC 18-0682 (KIB018A), KUMCC 18-0164 (KIB018B).


GenBank numbers: ITS = MK214368, LSU = MK214371, SSU = MK214374, TEF1-α = MK214377 (KUMCC 18-0163); ITS = MK359434, LSU = MK359439, SSU = MK359444 TEF1-α = MK359073 (KUMCC 18-0164).


Notes: Keissleriella caraganae is similar to other Keissleriella species in having ascomata with an ostiolar neck, filled with short, brown, aseptate periphyses, bitunicate, broadly cylindrical to cylindric-clavate asci and septate ascospores, surrounded by distinct mucilaginous sheath. Multigene phylogenetic analyses show that K. caraganae is sister to K. yonaguniensis Kaz. Tanaka & K. Hiray. (KT2604). Keissleriella caraganae has ellipsoidal to fusiform, pale yellowish, 3-septate ascospores, whereas K. yonaguniensis has cylindrical, yellowish, 5-septate ascospores, with rounded ends.