Pleosporales » Incertae sedis » Crassiparies

Crassiparies quadrisporus

Crassiparies quadrisporus M. Matsum., K. Hiray. & Kaz. Tanaka, in Li et al., Fungal Diversity 78: 63 (2016)

Index Fungorum number: IF 815295; Facesoffungi number: FoF02025

Saprobic on dead woody twigs of Shorea assamica. Sexual morph: Ascomata 160–360 μm high × 270–420 μm diam., (x̅ = 240 × 340 μm, n = 6), scattered, solitary, sometimes in groups of 2–3, globose to subglobose, immersed to partially erumpent through the host tissues, black, with a central ostiole. Peridium 19–26 μm wide, comprising two types of cell layers, outer layer brown, inner layer comprising hyaline, thick walled cells of textura angularis. Hamathecium comprising 1.5–2.5 μm wide, filiform, hyaline, septate, branched pseudoparaphyses embedded in a gelatinous matrix. Asci 80–120 × 18–23 μm (x̅ = 99 × 20 μm, n = 25), 4 spored, bitunicate, cylindric to broadly clavate, straight or slightly curved, apically rounded, with short truncated pedicel. Ascospores 30–33 × 9–13 μm (x̅ = 31.3 × 11.4 μm, n = 30), 1–2 seriate, hyaline, broadly fusiform, straight or slightly curved with rounded ends, 1 septate, constricted at the septum, smooth walled, large guttules in each cell. Asexual morph: Conidiomata pycnidial, scattered, globose to depressed globose, 170–530 μm high, 130–300 μm diam., ostiolate. Ostiolar neck 60–230 μm high, 60–130 μm diam., central, cylindrical to papillate, composed of rectangular to subglobose, thick-walled, 3–10 μm, dark brown cells. Conidiomatal wall 5–10 μm thick at the side, composed of rectangular to polygonal, thin-walled, 3.5–10 × 2–5 μm, pale brown cells. Paraphyses and conidiophores absent. Conidiogenous cells cylindrical to lageniform, 5.5–10 × 2–3.5 μm, phialidic. Conidia cylindrical, 6.5–14.5(–17) × 2.5–4 μm (mean ± SD = 10.2 ± 2.2 × 3.1 ± 0.3 μm, n = 50), l/w (2.2–)2.5–4.0(–4.5) (mean ± SD = 3.3 ± 0.6, n = 50), hyaline, aseptate, without sheath (Tanaka et al. 2017).

Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the basal and apical cell of ascospore. Colonies on PDA, reaching 40 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, fimbriate, atrovirens, dense, flat; reverse, atrovirens.

 

Material examined: China, Yunnan Province, Baoshan, on dead woody twigs of Shorea assamica (Dipterocarpaceae), 13 July 2020, G.C. Ren, BS22 (HKAS 122709), living culture KUMCC 21-0511.

 

Known distribution: On dead twigs of Acer sp. in Japan (Li et al. 2016), on dead woody twigs of Shorea assamica in China (This study).

 

GenBank numbers: LSU: OQ170861, ITS: OQ158939, tef1-α: OR613435.

Notes: Crassiparies quadrisporus was introduced by Li et al. (2016) based on the combined phylogeny of LSU and SSU sequence data. In the present study, a multi-gene phylogeny indicates that our strain of C. quadrisporus (KUMCC 21-0511) formed a sister clade with other strains of C. quadrisporus (HHUF 30409, KH111, HHUF 30590) with 100% ML bootstrap support and 1.00 BYPP value. Our collection (KUMCC 21-0511) is similar to C. quadrisporus (HHUF 30409, HHUF 30590) in having 4-spored, bitunicate, cylindric to broadly clavate asci and hyaline, broadly fusiform, 1-septate ascospores (Li et al. 2016, Tanaka et al. 2017). Crassiparies quadrisporus is a saprobic on dead twigs of Acer sp. (Sapindaceae) and Machilus japonica (Lauraceae) in Japan (Li et al. 2016, Tanaka et al. 2017). Therefore, we report our saprobic collection KUMCC 21-0511 as the first record of Crassiparies quadrisporus on the woody litter of Shorea assamica in China.

 

 

Figure 1. Crassiparies quadrisporus (HKAS 122709). a Material examined. b Appearance of ascomata on the host substrate. c Section of an ascoma. d Peridium. e Pseudoparaphyses. f–i Asci. j–m Ascospores. n, o Culture characters on PDA (n = from above, o = from below). Scale bars: c = 200 μm, d, f–i = 30 μm, e = 10 μm, j–m = 20 μm, n, o = 20 mm.

 

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

 

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