Pleosporales » Didymosphaeriaceae » Spegazzinia

Spegazzinia jinghaensis

Spegazzinia jinghaensis G.C. Ren and K.D. Hyde, Biology 11(no. 1660): 16 (2022)

Index Fungorum number: IF559809; FacesofFungi number: FoF 10707.

Saprobic on deadwoody twigs Myristica yunnanensis. Sexualmorph: undetermined. Asexual morph: Hyphomycetous. Sporodochia dark, dense, dry, powdery, velvety, 2–3 mm in diameter. Conidiogenous cells basauxic, ampulate, 5–6 µm high × 4–5 µm wide (  = 5.5 × 4.5 µm; n = 10), subspherical, hyaline-to-light-brown. Conidiophores of α conidia up to 80–120 × 1.4–2.0 µm (   = 100 × 1.7 µm, n = 10), erect or flexuous, unbranched, dark brown. Conidiophores of conidia 3.5–8 × 2.5–3.5 µm (    = 5.2 × 3 µm, n = 10) short, erect, unbranched, sub-hyaline or light brown. α conidia 16–20 ×15–19 µm (   = 17.9 × 17.5 µm; n = 20), 4-celled, stellate-shaped, brown-to-dark-brown, each cell globose to subglobose with dark brown warts on the surface of the cells, conspicuous spines 3.5–8 × 1–2 µm ( = 6 × 1.4 µm; n = 15), deeply constricted at the septa. b conida 12–16 × 13–17.5 µm ( = 14.3 × 15 µm; n = 30), 4-celled, disc-shaped, quadrangular or subspherical, initially pale brown, becoming brown-to-dark-brown at maturity, each cell turbinate, crossed septate, the cross-septate partly brown, smooth to verrucose, sometimes cells have raised verrucose around their edges, deeply constricted at the septa, flat from the side view, frequently with attached conidiogenous cells when splitting from the conidiophores.

Culture characteristics: The colonies on PDA reached 30–40 mm diam. at 14 days at room temperature (25–30 ºC), superficial, circular, rough surface, gray on the base, with sparse white mycelia on the surface; reverse black.

Material examined: China, Yunnan Province, Xishuangbanna Dai Autonomous Prefecture, Jinghong, Jingha (21º78.06´ N, 101º05.61´ E), on dead woody twigs of Myristica yunnanensis, 4 March 2020, G. C. Ren, JHD24 (HKAS 122787, holotype), ex-type culture, KUMCC 21-0495; ibid., JHD25 (HKAS 122878, isotype), living culture, KUMCC 21-0496.

Notes: Spegazzinia jinghaensis is introduced as a new species based on its distinctmorphology and the combined phylogeny of SSU, LSU, ITS, and tef 1-α. In the phylogenetic analyses, S. jinghaensis is distinct from other sequenced species within this genus and closely related to S. bromeliacearum (URM 8084) and S. intermedia (CBS 249.89) with strong statisticalsupport (78% ML bootstrap and 1.00 BYPP, Figure 1). Spegazzinia jinghaensis differs from S. bromeliacearum and S. intermedia in having two types of conidia (stellate-shaped conidia: 17.9 ×17.5 µm and disc-shaped conidia: 14.3 × 15 µm). In contrast, S. bromeliacearum has globose conidia (26.5–28 µm diam.) with spines, and S. intermedia has disc-shaped conidia (18–28 µm diam.), which are dentate at the margin.

 

Figure 1. Phylogram generated from ML analysis based on the combined SSU, LSU, ITS, and tef 1- dataset. Bootstrap support values for ML equal to or higher than 75%, and BYPP equal to or greater than 0.90 are shown above the nodes.

 

 

Figure 2. Spegazzinia jinghaensis (HKAS 122787, holotype). (a,b) Fungal colonies on the host surface; (c–e) conidiophore of a conidia and a conidia; (f,g) α conidia; (h–l)  conidia; (m,n) germinated conidia (m a conidium, n  conidium); (o,p) culture characters on PDA. Scale bars, (c–e) 20 µm; (f,g) 15 µm; (h–l) 10 µm; (m,n) 20 µm; (o,p) 30 mm.

 

References

1. Ren GC, Wanasinghe DN, de Farias ARG, Hyde KD et al. (2022) Taxonomic Novelties of Woody Litter Fungi (Didymosphaeriaceae, Pleosporales) from the Greater Mekong Subregion. Biology, 11, 1660, Doi /10.3390/biology11111660.

 

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