Pleosporales » Didymosphaeriaceae » Pseudopithomyces

Pseudopithomyces pandanicola

Pseudopithomyces pandanicola Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [25] (2018)

Index Fungorum number: IF554483            Facesoffungi number: FoF04494

 

Saprobic on dead leaves of Pandanus amaryllifolius. Colonies effuse, dark brown to black. Mycelium mostly superficial or partly immersed on the substrate, composed of septate, branched, smooth, thin-walled, hyaline hyphae. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Conidiophores micro- to macronematous, mononematous, hyaline. Conidiogenous cells holoblastic, terminal, hyaline, cylindrical. Conidia 10–25 × 7–15 μm ( x̄ = 19 × 11 μm, n = 20), muriform with 2–3 transverse septa and 1–2 longitudinal septa, verruculose to echinulate, amygdaliform or ovoid, yellow to brown, often carrying part of broken conidiogenous cell at base.

 

Culture characteristics: Conidia germinating on MEA within 12 hr. Colonies on MEA, grey on the surface, with dense, circular, with entire edge, raised, dark brown in reverse, with smooth margin.

 

Material examined: THAILAND, Chiang Rai Province, Mueang District, Mae Fah Luang University, on Pandanus amaryllifolius Roxb., 15 December 2017, S. Tibpromma P10 (MFLU 18-0029, holotype); ex-type living culture, MFLUCC 18-0116, GenBank numbers LSU: MH376738; ITS: MH388364; SSU: MH388329; TEF1: MH388399; RPB2: MH412734; TUB2: MH412724.

 

Notes: Pseudopithomyces pandanicola clusters with P. kunmingnensis Karun. & K.D. Hyde and P. chartarum (Berk. & M.A. Curtis) Jin F. Li, Ariyaw. & K.D. Hyde. However, P. kunmingnensis has globose or subglobose conidiogenous cells with verruculose to echinulate and light brown to brown conidia (Hyde et al. 2017), while P. chartarum has broadly ellipsoid, mid brown to dark brown conidia (Ariyawansa et al. 2015). In a blastN search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 18-0116 is P. chartarum with 100% identity to the strain OA28 (JQ406588), while the closest matches with the RPB2 sequence were with 99% identical P. chartarum strain UTHSC 04-678 (LK936414).

 

Figure X. Pseudopithomyces pandanicola (MFLU 18-0029, holotype). a Colonies on dead leaves of Pandanus amaryllifolius. b-g Conidiogenous cells and conidia. h Germinating conidium. i, j Colony on MEA from above and below. Scale bars: a-e, g, h = 5 μm, f = 10 μm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, et al. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 92:1–160.

 

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