Chromolaenicola sapindi
Chromolaenicola sapindi G.C. Ren and K.D. Hyde, Biology 11(no. 1660): 13 (2022)
Index Fungorum number: IF 559806. FacesofFungi number: FoF 10704.
Saprobic on dead woody twigs of Sapindus rarak. Sexual morph: Ascomata 420–530 µm high × 270–350 µm diam. (
480 × 300 µm, n = 5), immersed-to-erumpent, solitary or scattered, coriaceous, ampulliform or obovoid, dark brown. Ostiole central. Peridium 15–25 µm thick, 4–7-layered, comprising pale-brown-to-brown cells of textura angularis. Hamathecium 1.5–3 µm wide, comprising cylindrical, septate, branching pseudoparaphyses, embedded in a hyaline, gelatinous matrix. Asci 125–155 × 12–16 µm ( = 138 ×13 µm, n = 20), bitunicate, 8-spored, cylindrical-clavate, straight, slightly curved at the end, apically rounded, with a pedicel (7–10 µm long). Ascospores 16–23 × 6.5–9.5 µm ( = 18.9 × 8 µm, n = 30), overlapping 1-seriate, ellipsoidal, initially hyaline-to-pale-brown and aseptate or 1-septate, guttulate, becoming reddish-brown-to-brown, and 1-septate at maturity, slightly constricted at the central septum, with or without guttules, thick and smooth-walled, without a gelatinous sheath. Asexual morph: undetermined.
Culture characteristics: The colonies on PDA reached 20–30 mm diam. after 14 days at room temperature (25–30 
), superficial, circular, umbonate at the center, with dense mycelia, smooth, downy, velvety, fimbriate, white; reverse white at the margin, dark brown at the center.
Material examined: China, Yunnan Province, Lancang, Lahu Autonomous Prefecture, Hani (22º24.381´ N, 100º06.647´E, elevation 900 m), on dead woody twigs of S. rarak, 23 March 2020, G.C. Ren, LGY32 (HKAS 122789, holotype), ex-type culture KUMCC 21-0564; ibid., LGY33 (HKAS 122876, isotype), living culture KUMCC 21-0594.
Notes: Chromolaenicola sapindi is introduced as a newly discovered species based on its distinct morphology and analysis of a combined SSU, LSU, ITS, and tef 1-
dataset. Our samples (KUMCC 21-0564 and KUMCC 21-0594) were clustered with other Chromolaenicola species with 84% ML bootstrap and 0.91 BYPP support. Our species can be distinguished from C. nanensis and C. thailandensis in having 2-celled, guttulate ascospores. Both C. nanensis and C. thailandensis have muriform ascospores with 3-transverse septa and 1-vertical septum when mature. We did not obtain the asexual morph from C. sapindi. Therefore, the morphological comparison between our new species and other Chromolaenicola species known only in their asexual morph was not possible. However, based on the phylogenetic distinctiveness, C. sapindi is introduced as a new species.
Figure 1. Phylogram generated from ML analysis based on the combined SSU, LSU, ITS, and tef 1- 
dataset. Bootstrap support values for ML equal to or higher than 75%, and BYPP equal to or greater than 0.90 are shown above the nodes.
Figure 2. Chromolaenicola sapindi (HKAS 122789, holotype). (a,b) Appearance of ascomata on host substrate; (c) section of ascoma; (d) peridium; (e) hamathecium; (f‒i) asci; (j‒o) ascospores; (p) germinated ascospore; (q,r) culture characters on PDA (q from above, r from below). Scale bars, (c) 200 μm; (d,f‒i) 50 μm; (e,j‒p) 10 μm; (q,r) 30 mm.
References
- Ren GC, Wanasinghe DN, de Farias ARG, Hyde KD et al. (2022) Taxonomic Novelties of Woody Litter Fungi (Didymosphaeriaceae, Pleosporales) from the Greater Mekong Subregion. Biology, 11, 1660, Doi /10.3390/biology11111660.
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