Camarosporidiella xianggelilaensis
Camarosporidiella xianggelilaensis G.C. Ren & K.D. Hyde, in Ren, Jayasiri, Tibpromma, Farias, Chethana, Faraj, Wanasinghe, Xu, Hyde & Gui, Mycosphere 15(1): 998 (2024)
Index Fungorum number: IF 901349; Facesofungi number: FoF 13878
Saprobic on dead twigs of Rhododendron rubiginosum. Sexual morph: Ascomata 480–620 μm high, 560–690 μm diam., (x̅= 530 × 610 μm, n = 5), black, superficial to semi immersed, gregarious, subglobose to globose, coriaceous, clustered beneath the host periderm, unilocular, with a central ostiole. Peridium 50–160 μm wide, thin at the base, thick at the sides, composed of dark brown outer layers and inner layers comprising hyaline cells of textura angularis, thick walled. Hamathecium 2.5–3.3 μm wide, comprising filamentous, branched, septate pseudoparaphyses embedded in a gelatinous matrix. Asci 200–290 × 15–17.5 μm (x̅ = 241 × 16 μm, n = 20), 4–8 spored, bitunicate, fissitunicate, cylindrical, slightly curved, with a short furcate to truncate pedicel, apically rounded. Ascospores 37–50 × 9.6–12.6 μm (x̅ = 42 × 11 μm, n = 30), uniseriate, muriform, fusiform, conical and pointed at the ends, 6−9 transversely septate, with 1−2 vertical septa, slightly constricted at the middle septum, slightly curved or straight, initially hyaline, becoming brown when mature, thick-walled, guttulate, not surrounded by a mucilaginous sheath. Asexual morph: Undetermined.
Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from ascospores .Colonies on PDA, reaching 30 mm diameter after two weeks at 20–25 ℃, mycelia superficial, irregular or rhizoid colonies, filamentous, flat, surface rough with rhizoid edge, grey with greyish white spots; reverse, black.
Material examined: China, Yunnan Province, Diqing, Xianggelila, on dead woody twigs of Rhododendron rubiginosum (Ericaceae), 1 September 2020, G.C. Ren, TM13 (HKAS 122771, holotype), ex-type living culture KUMCC 21-0661.
GenBank numbers: SSU: OQ168202, LSU: OQ170846, ITS: OQ158926, tef1-α: OR613427.
Notes: Our phylogenetic analyses show that Camarosporidiella xianggelilaensis (KUMCC 21-0661) clustered sister to ten strains of C. elaeagnicola with 55% ML bootstrap support and 0.75 BYPP value. Camarosporidiella xianggelilaensis was recorded from its sexual morph, while C. elaeagnicola has only been reported from its asexual morph from Elaeagnus angustifolia in Russia (Wanasinghe et al. 2017). Therefore, we are unable to compare the morphologies between these two species. Camarosporidiella xianggelilaensis shared similar morphology with other sexual morphs of Camarosporidiella in having superficial to semi-immersed, gregarious, globose, black, unilocular conidiomata; thick-walled peridium; branched, septate hamathecium; bitunicate, fissitunicate, cylindrical asci, uniseriate, muriform, fusiform ascospores (Wanasinghe et al. 2017) however it differed in having 4–8-spored asci. In the sequence comparison for the ITS region between Camarosporidiella xianggelilaensis (KUMCC 21-0661) and C. elaeagnicola (MFLUCC 14-0908) showed a 2.1% (11/523 bp, without gaps) base pair difference in ITS region, 1.3% (12/923 bp, without gaps) base pair difference in the tef1-α region. Therefore, based on morphological characterization and phylogenetic analyses, Camarosporidiella xianggelilaensis is introduced as a new species.
Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, and tef1-α sequence data, representing Camarosporidiellaceae. Related sequences are obtained following Hyde et al. (2020b). Ninety-nine strains are included in the combined analyses, which comprise 3310 characters for SSU, LSU, ITS, and tef1-α alignment. Two strains of Staurosphaeria lycii (MFLUCC 17-0210 and MFLUCC 17-0211) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -7756.134870 is presented. The matrix had 353 distinct alignment patterns with 7.05% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.242018, C = 0.243119, G = 0.267054, T = 0.247809; substitution rates AC = 1.456305, AG = 3.573390, AT = 2.277000, CG = 0.814859, CT = 7.520960, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.
Figure 2. Continued.
Figure 3. Camarosporidiella xianggelilaensis (HKAS 122771, holotype). a, b Appearance of ascomata on the host substrate. c Section of an ascoma. d Peridium. e Pseudoparaphyses. f–j Asci. k–q Ascospores. r Germinated ascospore. s, t Culture characters on PDA (s = from above, t = from below). Scale bars: c = 200 μm, d = 100 μm, e = 20 μm, f–j = 50 mm, k–r = 30 mm, s, t = 20 mm.
References
- Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8
Recent Genus
AmphisphaeriaParaeutypella
Halorosellinia
Recent Species
Torula chromolaenaePericonia delonicis
Neopestalotiopsis theobromicola