Pleosporales » Bambusicolaceae » Corylicola

Corylicola italica

Corylicola italica Wijesinghe, Camporesi, Yong Wang bis & K.D. Hyde, Biodiversity Data Journal 8 (e55957): 8 (2020)

 

Index Fungorum number: IF 557768; Facesofungi number: FoF 08685

 

Saprobic on dead woody twigs of Quercus kingiana. Sexual morph: Ascomata 95–170 μm high × 150–180 μm diam., (x̅ = 127 × 164 μm, n = 5), immersed to erumpent, solitary or scattered, coriaceous, globose to subglobose, uni loculate, black. Ostioles central, minute papilla. Peridium 7–16 μm wide, 3–4-layered, comprising brown cells of textura angularis. Hamathecium 1.5–2.7 μm wide, comprising cylindrical, branched, septate, cellular pseudoparaphyses embedded in a hyaline gelatinous matrix. Asci 53–65 × 5.6–6.8 μm (x̅ = 56 × 6.1 μm, n = 20), 8 spored, bitunicate, cylindrical clavate, straight or curved, apically rounded, short pedicellate, furcate pedicels, with a minute ocular chamber. Ascospores 8.5–11 × 2.7–3.4 μm (x̅ = 10 × 3 μm, n = 30), uniseriate, ellipsoidal, initially hyaline to pale brown, 1 septate, becoming brown at maturity, constricted at the septum, the cells above central septum often broader than the lower ones, guttulate, thick and smooth walled, without a gelatinous sheath. Asexual morph: Coelomycetous. Conidiomata 175–200 high 150–170 μm diam., (x̅ = 183 × 161 μm) pycnidial, solitary to gregarious, scattered, semi-immersed to superficial, visible as black spore mass surrounded by cellular vegetative hyphae (1–2 μm width), globose to subglobose, glabrous, uniloculate to multi-loculate, ostiolate. Ostiolate 45–50 μm long, 50–60 μm wide, central and circular. Conidiomata wall 7–20 μm wide, composed of several layers of pale to dark brown, pseudoparenchymatous cells, outermost layers comprising 3−5 layers of dark brown cells of textura prismatica to textura angularis, inner layers comprising 2−3 layers of pale brown to hyaline cells of textura angularis. Conidiophores reduced to conidiogenous cells, originated from the basal cavity of conidiomata. Conidiogenous cells 3–4.5 × 2–4 μm (x̅ = 3.6 × 3 μm, n = 30), holoblastic, phialidic, ampulliform, yellowish to pale brown, aseptate, smooth-walled. Conidia 3–5 × 2–3 μm (x̅ = 4 × 2.5μm, n = 30), solitary, globose or oblong to ellipsoid, rounded or obtuse ends, yellowish to pale brown, aseptate, rarely guttulated, one-celled, smooth-walled (Wijesinghe et al. 2020).

 

Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the apical cell of the ascospore Colonies on PDA, reaching 15 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, dense, flat, lobate edge, raised at the center, white, granular and circular crack on the surface; reverse, white at edge, grayish white at center.

 

Material examined: China, Yunnan Province, Lancang, Lahu Autonomous Prefecture, Hani, on dead woody twigs of Quercus kingiana (Fagaceae), 20 July 2020, G.C. Ren, LGY31 (HKAS 122733), living culture KUMCC 21 0563; ibid., LGY16 (HKAS 122731), living culture KUMCC 21 0553; ibid., LGY22 (HKAS 122732), living culture KUMCC 21 0559; China, Yunnan Province, Lincang, on dead woody twigs of Cryptocarya hainanensis (Lauraceae) LC22 (HKAS 122734) living culture KUMCC 21-0605.

 

Known distribution: On dead hanging branch of Corylus avellana (Betulaceae) in Italy (Wijesinghe et al. 2020), dead woody twigs of Quercus kingiana (Fagaceae) and Cryptocarya hainanensis (Lauraceae) in China.

 

GenBank numbers: KUMCC 21-0563: LSU: OQ170841, ITS: OQ158921, SSU: OQ168198, tef1-α: OR613421, rpb2: OR578553; KUMCC 21-0553: LSU: OQ170839, ITS: OQ158919, SSU: OQ168196; KUMCC 21-0559: LSU: OQ170840, ITS: OQ158920, SSU: OQ168197, tef1-α: OR613422, rpb2: OR578554; KUMCC 21-0605: LSU: OQ170842, ITS: OQ158922, SSU: OQ168199, tef1-α: OR613423, rpb2: OR578555.

 

Notes: Multi-gene phylogenetic analyses show that four strains of Corylicola italica (KUMCC 21 0563, KUMCC 21 0553, KUMCC 21 0559 and KUMCC 21 0605) grouped with type strains of Corylicola italica (MFLU 19 0500 and MFLUCC 20 0111) (Fig. 20). In a BLASTn search of NCBI GenBank, the closest match of the ITS sequences showed that the strains (KUMCC 21 0563, KUMCC 21 0553 and KUMCC 21 0559) are identical to Corylicola italica with 100% similarity (except KUMCC 21 0605). The holotype of C. italica and the new collections both have uni loculate ascomata, central ostioles with minute papilla, cellular pseudoparaphyses, and cylindrical asci with short, furcate pedicels with single septate, brown ascospores. Therefore, we identified our four collections as Corylicola italica. The collections are introduced here as the first records from Quercus kingiana (Fagaceae) and Cryptocarya hainanensis (Lauraceae) from China.

 

 

Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, tef1-α, and rpb2 sequence data, representing Bambusicolaceae. Related sequences are obtained following Phukhamsakda et al. (2022). Forty two strains are included in the combined analyses, which comprise 4040 characters for SSU, LSU, ITS, tef1-α, and rpb2 alignment. Murilentithecium clematidis (MFLUCC 14 0561) and M. lonicerae (MFLUCC 18 0675) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of 17373.628804 is presented. The matrix had 939 distinct alignment patterns, with 17.41% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.242291, C = 0.257231, G = 0.271517, T = 0.228961; substitution rates AC = 1.354747, AG = 2.926058, AT = 1.006416, CG = 0.994544, CT = 6.807971, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.

 

 

Figure 2. Corylicola italica (HKAS 122733). a Material examined. b Appearance of ascomata on the host substrate. c Section of ascoma. d Peridium. e Pseudoparaphyses. f–h Asci.i–n Ascospores. o Germinated ascospores. p, q Culture characters on PDA (p = from above, q = from below). Scale bars: c = 100 μm, d = 30 μm, e, o = 10 μm, f–h = 20 μm, i–n = 5 μm, p, q = 30 mm.

 

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

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