Pleosporales » Anteagloniaceae » Anteaglonium

Anteaglonium parvulum

Anteaglonium parvulum (Schwein.) Mugambi & Huhndorf, Syst. Biodiv. 7(4): 460 (2009) Facesoffungi number: FoF 01931

 

Sexual morph: Saprobic on dead wood. Sexual morph: Ascomata 128–192 µm high × 134–304 µm diameter ( = 223 × 166.5 µm), hysterothecial, superficial, semi-immersed or sunken at the base, oval to elongate, or subglobose, black, carbonaceous, straight or curved, with slightly hyphae penetrating into the host tissue. Ostiole central, with longitudinal slit. Peridium 34–58 μm thick, relatively thick, strongly carbonaceous texture, composed of thick-walled dark angular or relatively compressed pseudoparenchymatous cells, inner layers composed of hyaline to brown cells of textura angularis. Hamathecium comprising 1–1.5 μm thin, cylindrical to filiform, hyaline, branched, septate, anastomosing pseudoparaphyses. Asci 34–53 × 4–5.5 μm ( = 44 × 4.5 µm, n = 20), 8-spored, bitunicate, fissitunicate, elongate cylindric-clavate, straight or slightly curved, sessile or with short pedicel, apically rounded, with a minute ocular chamber. Ascospores 5–8 × 2.5–3 μm ( = 6 × 3 μm, n = 20), 1-seriate, hyaline, ellipsoidal, 1-septate, constricted at the septa, upper cell wider and tapering towards the narrow ends, guttulate, smooth-walled. Asexual morph: Coelomycetous. Conidiomata 42–85 μm high, 42–77 μm diameter, superficial, grouped to scattered, subiculum, uniloculate or multi-loculate, subglobose to globose, dark brown. Conidioma wall 42–77 μm wide, pseudoparenchymatous, composed of dark brown cells of textura angularis to subglobosa. Conidiophores 11–21 μm long, 2–2.5 μm wide, cylindrical, hyaline. Conidiogenous cells phialidic, hyaline, simple, smooth, with a conspicuous collarette at the apex. Conidia 3–4 × 1.5–2 μm (= 4 × 2 μm, n = 20), 1-celled, oblong to ellipsoidal or oval, rounded ends, slightly curved, aseptate, hyaline, smooth-walled.

 

Culture characteristics: Ascospores germinating on MEA with 12 h and germ tubes produced from both ends. Colonies on MEA reaching 9 mm after 7 days at 28 °C, slightly effuse, radially with undulate edge, greyish to light brown, with reddish pink pigmented in media. Mycelium superficial, branched, septate, hyaline to light brown, smooth-walled, asexual state formed within 60 days.

 

Habitat: on dead wood (Hyde et al. 2020).

 

Known distribution: USA, Louisiana and Michigan States (Mugambi & Huhndorf 2009), India (Hongsanan et al. 2020), Thailand, Chiang Rai Province (Jayasiri et al. 2016, Hyde et al. 2020).

 

Material examined: Thailand, Chiang Rai Province, Huai Kang Pla, on dead wood, 25 October 2010, S. Boonmee, HPK03 (MFLU 10–0974, new geographical record); living culture (MFLUCC 10– 0928 = BCC 52146).

 

GenBank Accession No: ITS: MN608542, LSU: MN577411, SSU: MN577422.

 

Notes: The new collection is a typical Anteaglonium species, and it shares similar morphological characters such as hysterothecial ascomata, black, carbonaceous, cylindric-clavate, bitunicate, fissitunicate asci and uniseriate, ellipsoidal, hyaline, 1-septate, small ascospores (less than 10 μm). In addition, this species always developed a coelomycetous asexul morph in culture (Fig. 6). Multi-gene phylogenetic analysis placed these four strains (MFLUCC 10–0928, MFLUCC 11–0374, MFLUCC 11–0380, MFLUCC 11–0511) with other Anteaglonium parvulum isolates (SMH 5210, MFLUCC 14–0815, MFLUCC 14–0817, MFLUCC 14–0821, MFLUCC 14–0823) with moderate-support and type sequence of this species is unavailable (Hyde et al.2020). These four strains share similar sexual morphological features, but they differ in culture and asexual characteristics.