Pleosporales » Anteagloniaceae » Anteaglonium

Anteaglonium parvulum

Anteaglonium parvulum (Schwein.) Mugambi & Huhndorf, Syst. Biodiv. 7(4): 460 (2009) Facesoffungi number: FoF 01931

 

Sexual morph: Ascomata 155–261 µm high × 180–284 µm diameter (x̄ = 196 × 240 µm), hysterothecial, superficial, semi-immersed or sunken at the base, oval to elongate, or subglobose, uniloculate or multi-loculate, carbonaceous, brittle, straight or curved, black. Ostiole central, with longitudinal slit. Peridium 32–48(–77.5) μm thick, relatively thick, strongly carbonaceous texture, composed of thick-walled dark angular or relatively compressed pseudoparenchymatous cells, inner layers composed of hyaline to brown cells of textura angularis. Hamathecium comprising 1–2.5 μm thin, cylindrical to filiform, hyaline, branched, septate, anastomosing pseudoparaphyses. Asci 28–41 × 4–5.5 μm (x̄ = 35.5 × 4.5 µm, n = 20), 8-spored, bitunicate, fissitunicate, elongate cylindric-clavate, straight or slightly curved, sessile or with short pedicel, apically rounded, with a minute ocular chamber. Ascospores 5–8 × 2–3 μm (x̄ = 6.5 × 2.5 μm, n = 20), 1-seriate, hyaline, ellipsoidal, 1-septate, constricted at the septa, upper cell wider and tapering towards the narrow ends, guttulate, smooth-walled. Asexual morph: Coelomycetous. Conidiomata grouped to scattered, superficial, semi-immersed at the base, subglobose to globose, dark brown. Conidioma wall pseudoparenchymatous, composed of dark brown cells of textura angularis. Conidiophores 9–21 μm long, 1.5–3 μm wide, cylindrical, hyaline. Conidiogenous cells holoblastic. Conidia 2.5–4 × 2–3 μm (x̄ = 3 × 2 μm, n = 20), 1-celled, globose, subglobose to ellipsoidal, aseptate, hyaline, guttulate, smooth-walled.

 

Culture characteristics: Ascospores germinating on MEA with 12 h and germ tubes produced from both ends. Colonies on MEA reaching 8 mm after 7 days at 28 °C, slightly effuse, radially with undulate edge, greyish, light brown, reddish pink. Mycelium superficial, branched septate, hyaline to light brown, with reddish pink pigmented, smooth-walled, asexual state formed within 60 days.

 

Habitat: on dead wood (Hyde et al. 2020).

 

Known distribution: USA, Louisiana and Michigan States (Mugambi & Huhndorf 2009), India (Hongsanan et al. 2020), Thailand, Chiang Rai Province (Jayasiri et al. 2016, Hyde et al. 2020).

 

Material examined: Thailand, Chiang Rai Province, Muang District, Doi Pui, on dead wood, 10 May 2011, S. Boonmee, DP05 (MFLU 11–0389, new geographical record); living culture (MFLUCC 11–0380 = BCC 52031).

 

GenBank Accession No: ITS: MN608544, LSU: MN577413, SSU: MN577424.

 

Notes: The new collection is a typical Anteaglonium species, and it shares similar morphological characters such as hysterothecial ascomata, black, carbonaceous, cylindric-clavate, bitunicate, fissitunicate asci and uniseriate, ellipsoidal, hyaline, 1-septate, small ascospores (less than 10 μm). In addition, this species always developed a coelomycetous asexual morph in culture (Fig. 6). Multi-gene phylogenetic analysis placed these four strains (MFLUCC 10–0928, MFLUCC 11–0374, MFLUCC 11–0380, MFLUCC 11–0511) with other Anteaglonium parvulum isolates (SMH 5210, MFLUCC 14–0815, MFLUCC 14–0817, MFLUCC 14–0821, MFLUCC 14–0823) with moderate-support and type sequence of this species is unavailable (Hyde et al. 2020). These four strains share similar sexual morphological features, but they differ in culture and asexual characteristics.

 

Figure X. Anteaglonium parvulum (MFLU 11–0389, new geographical record). a Appearance of ascomata on woody substrate. b, c Vertical and longitudinal sections of multi-loculate stromata. d Peridium. e Pseudoparaphyses. f–h Asci (Fig. h stained in Melzer's reagent). i–k Ascospores. Scale bars: a = 1000 µm, b, c = 100 µm, d = 40 µm, e–h = 10 µm, i–k = 5 µm.

 

Figure X. Anteaglonium parvulum (MFLUCC 11–0380, living culture) a Germinating spore. b, c Culture colonies on MEA from surface and reverse. d, e Aerial hyphae in culture. e Compressed hyphae and developing conidiomata in culture. f Squash mount of conidioma. g, h Developing of conidiophores and conidiogenous cells. i Conidia. Scale bars: a, d, g, h = 10 µm, e = 20 µm, f = 50 µm, i = 5 µm.

 

Reference:

1. Hongsanan S, Hyde KD, Phookamsak R,Wanasinghe DN et al. 2020 – Refined families of Dothideomycetes. Refined families of Dothideomycetes: Dothideomycetidae and Pleosporomycetidae. Mycosphere 11(1), 1553–2107.
2. Mugambi GK, Huhndorf SM. 2009 – Parallel evolution of hysterothecial ascomata in ascolocularous fungi (Ascomycota, Fungi). Systematics and Biodiversity 7, 453–464.
3. Hyde KD, de Silva NI, Jeewon R, Bhat DJ, et al. 2020 – AJOM new records and collections of fungi: 1-100. Asian Journal of Mycology 3(1), 22–294.
4. Jayasiri SC, Jones EBG, Kang JC, Promputtha I et al. 2016 – A new species of genus Anteaglonium (Anteagloniaceae, Pleosporales) with its asexual morph. Phytotaxa 263, 233–244.

 

 

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