Anastomitrabeculia xishuangbannaensis
Anastomitrabeculia xishuangbannaensis G.C. Ren & K.D. Hyde, in Ren, Jayasiri, Tibpromma, Farias, Chethana, Faraj, Wanasinghe, Xu, Hyde & Gui, Mycosphere 15(1): 986 (2024)
Index Fungorum number: IF 901348; Facesoffungi Number: FoF13875
Saprobic on dead woody twigs of Knema furfuracea. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 500–570 μm high × 450–650 μm diam., (x̅ = 550 × 570 μm, n = 5), solitary, scattered, immersed beneath the host epidermis, coriaceous, globose to subglobose, brown to dark-brown, ostiolate. Ostiole 230–540 μm long, 110–160 μm diam. (x̅ = 400 × 130 μm, n = 5), centrally located, filled with hyaline cells. Conidiomata wall 21–30 μm wide, composed several layers of thick-walled, dark brown to brown outer layer and inner layer comprising hyaline cells of textura angularis. Paraphyses 2.5–3.8 μm wide, cylindrical to fusiform, septate, branched. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 10–32 × 4–11 μm (x̅ = 23 × 7 μm, n = 10), enteroblastic, phialidic, determinate, discrete, cylindrical or ampulliform, hyaline, smooth-walled, arising from stratum. Conidia 42–50 × 21–30 μm (x̅ = 45 × 26 μm, n = 30), oval to obovoid, dictyosporous, muriform, initially hyaline, becoming brown to dark brown at maturity, multi-septate, sectored.
Culture characteristics: Conidia germinating on PDA within 24 h at room temperature (25°C). Germ tubes produced from around the conidia. Colonies on PDA, reaching 55 mm diameter after two weeks at 20–25 ℃, mycelia superficial, surface smooth, circular, velvety, fluffy, dense, white at margin, grey at centre; reverse, white at margin, dark grey at the centre.
Material examined: China, Yunnan Province, Xishuangbanna, on dead woody twigs of Knema furfuracea (Myristicaceae), 4 March 2020, G.C. Ren, JH42 (HKAS 122741, holotype), ex-type living culture KUMCC 21-0585. ibid., JH43, KUMCC 21-0586.
GenBank numbers: KUMCC 21-0585: LSU: OQ170834, ITS: OQ158914, SSU: OQ168194, tef1-α: OR613420; KUMCC 21-0586: LSU: OQ170835, ITS: OQ158915, SSU: OQ168195.
Notes –In our phylogenetic analysis, Anastomitrabeculia xishuangbannaensis clustered as a sister clade to type strain of A. didymospora with 100% ML bootstrap support and 1.00 BYPP value. Sequence comparison between A. didymospora (MFLUCC 16-0412) and A. xishuangbannaensis (KUMCC 21-0585) showed a 17.58% (80/455 bp, without gaps) base pair difference in the ITS region, 4.87% (40/821 bp, without gaps) base pair difference in the LSU region, 17.06% (138/809 bp, without gaps) base pair difference in tef1-α region. Anastomitrabeculia didymospora was introduced from its sexual morph (Bhunjun et al. 2021), while we introduced A. xishuangbannaensis from its asexual morph. Therefore, we were unable to compare our new species and A. didymospora. However, based on the phylogenetic distinctiveness, Anastomitrabeculia xishuangbannaensis is introduced as a new species, and our species is the first asexual morph recorded in this genus.
Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS and tef1-α sequence data. Related sequences are obtained following Bhunjun et al. (2021) and BLAST search results in GenBank. Sixty strains are included in the combined analyses, which comprise 3210 characters for SSU, LSU, ITS and tef1-α alignment. Arthonia dispersa (UPSC 2583) and Dendrographa decolorans (Ertz 5003) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -19749.079335 is presented. The matrix had 1152 distinct alignment patterns with 30.83% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.244104, C = 0.237282, G = 0.273441, T = 0.245174; substitution rates AC = 1.279169, AG = 2.990662, AT = 1.843852, CG = 0.776990, CT = 7.343522, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.
Figure 2. Anastomitrabeculia xishuangbannaensis (HKAS 122741, holotype). a Material examined. b Conidiomata on the natural wood surface. c, d Section through conidioma. e Ostiolar neck. f Conidioma wall. g Paraphyses. h, i Conidiogenous cells and developing conidia. j–o Conidia. p Germinated conidium. q, r Culture characters on PDA (q = from above, r = from below). Scale bars: d = 500 μm, e = 100 μm, f, h, i = 30 μm, g = 10 μm, j–o = 20 μm, p = 50 μm, q, r = 30 mm.
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