Pleosporales » Amorosiaceae » Angustimassarina

Angustimassarina kunmingensis

Angustimassarina kunmingense H.D. Yang & K.D. Hyde, Fungal Diversity 117: 18 (2023)

Index Fungorum number: IF662420 ; Facesofungi number: FoF11804

 

Saprobic on dead woody twigs of Quercus kingiana and Rhododendron rubiginosum. Sexual morph: Ascomata 180–300 μm high, 160–290 μm diam., (x̅ = 225 × 211 μm, n = 5), immersed under the host tissue, solitary or scattered, globose to subglobose, uni-loculate, coriaceous, dark brown, without papilla, and with a short central ostiole. Peridium 25–38 μm wide, 4–5-layered, composed dark brown cells of textura angularis to textura globulosa. Hamathecium comprising 1.7–2.9 μm wide, unbranched, septate, hyaline, cellular pseudoparaphyses, constricted at the septa, embedded in a gelatinous matrix. Asci 67–85 × 10–12 μm (x̅ = 74.6 × 10.6 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, slightly curved, with a truncated pedicel, apically rounded with a minute ocular chamber. Ascospores 19–23 × 4–5 μm (x̅ = 19.4 × 4.1 μm, n = 30), overlapping 1–2-seriate, hyaline, fusiform with tapering towards rounded ends, straight to slightly curved, 1(–3)-septate, constricted at the primary septum with asymmetric two cells, smooth-walled, large guttules in each cell, and surrounded by a large spreading sheath. Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the basal and apical cell of ascospore. Colonies on PDA, reaching 30 mm diameter after two weeks at 20–25 ℃, mycelia superficial, circular, fimbriate, dense, flat, entire edge, grayish white; reverse, atrovirens, white at the edge.

 

Material examined: China, Yunnan Province, Lancang, Lahu Autonomous Prefecture, Hani, on dead woody twigs of Quercus kingiana (Fagaceae), 19 July 2020, G.C. Ren, LGY01 (HKAS 122719), living culture KUMCC 21-0541; ibid., Diqing, Xianggelila, Nixi, on dead woody twigs of Rhododendron rubiginosum (Ericaceae), 1 September 2020, G.C. Ren, NX18 (HKAS 122763), living culture KUMCC 21-0643.

 

Known distribution: On dead aerial stem of Camellia semiserrata (Jayawardena et al. 2022), on dead woody twigs of Quercus kingiana (Fagaceae) and Rhododendron rubiginosum (Ericaceae) in China.

 

GenBank numbers: KUMCC 21-0541: LSU: OQ170832, ITS: OQ158912, SSU: OQ168192, tef1-α: OR613418; KUMCC 21-0643: ITS: OQ158913, LSU: OQ170833, SSU: OQ168193, tef1-α: OR613419.

 

Notes : Angustimassarina kunmingense was introduced by Jayawardena et al. (2022) from a dead aerial stem of Camellia semiserrata in China. Our collection (KUMCC 21-0541, KUMCC 21-0643) resemble A. kunmingense (KUMCC22-10799) in having globose to subglobose ascomata, cylindrical to cylindric-clavate asci (74.6 × 10.6 μm vs 68 × 8.1 μm) with a minute ocular chamber, and hyaline, fusiform ascospores (19.4 × 4.1 μm vs 20 × 3.5 μm) with 1(–3) septa (Jayawardena et al. 2022). In the phylogenetic analysis, our isolate (KUMCC 21-0541, KUMCC 21-0643) clustered with the ex-type strain of A. kunmingense (KUMCC 22-10799) with 99% ML bootstrap support and 1.00 BYPP value (Fig. 16). Sequence comparison for the ITS and tef1-α region between our isolates (KUMCC 21-0541, KUMCC 21-0643) and A. kunmingense (KUMCC22-10799) showed no significant base pair differences. Therefore, we introduce our collection as the first record of A. kunmingense from Quercus kingiana (Fagaceae) and Rhododendron rubiginosum (Ericaceae) in China.

 

 

Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, and tef1-α sequence data, representing Amorosiaceae. Related sequences are obtained following Jayasiri et al. (2019), Hyde et al. (2020b) and Jayawardena et al. (2022). Twenty-eight strains are included in the combined analyses, which comprise 3226 characters for SSU, LSU, ITS and tef1-α alignment. Lentimurispora urniformis (MFLUCC 18-0497) was used as the outgroup taxon. The best-scoring RAxML tree with a final likelihood value of -7833.074271 is presented. The matrix had 495 distinct alignment patterns with 31.58% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.240851, C = 0.246837, G = 0.270734, T = 0.241579; substitution rates AC = 0.915906, AG = 1.756917, AT = 1.538751, CG = 0.835070, CT = 8.179217, GT = 1.0000. The tree topology of ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.

 

 

Figure 2. Angustimassarina kunmingense (HKAS 122719). a Material examined. b Appearance of ascomata on the host substrate. c Section of an ascoma. d Peridium. e Pseudoparaphyses. f–i Asci. j–n Ascospores. o Germinated ascospore. p Ascospore in Indian ink showing sheath. q, r Culture characters on PDA (q = from above, r = from below). Scale bars: c = 250 μm, d = 50 μm, e = 20 μm, f–i = 30 μm, j–p = 10 μm, q, r = 30 mm.

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

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