Pleosporales » Amniculicolaceae » Amniculicola

Amniculicola guttulata

Amniculicola guttulata Z.L. Luo, K.D. Hyde & H.Y. Su, in Hyde et al., Fungal Diversity: 10.1007/s13225-019-00429-2, [13] (2019)

Index Fungorum number: IF555507         Facesoffungi  number: FoF05309


Sexual morph: Ascomata 290–320 µm high, 280–300 µm diam., solitary, scattered, or in small groups, superficial, coriaceous, with basal wall remaining immersed in host tissue, globose to subglobose, black, often laterally flattened, with a flattened base not easily removed from the substrate, mostly bearing remnants of wood fibres. Peridium 27–35 µm thick, 2-layered, outer layer composed of small, brown to dark brown, thick-walled cells of textura angularis, cells at apex smaller and  walls thicker, inner layer composed of hyaline, thin-walled cells of textura angularis. Hamathecium of dense, very long trabeculate pseudoparaphyses. Asci 113–127 × 9–11 µm ( = 120 × 10 µm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical, with a short, narrowed, twisted, bifurcate pedicel. Ascospores 23–27 × 5–7 µm ( = 25 × 6 µm, n = 25), obliquely uniseriate and partially overlapping, fusiform, with narrowly rounded to  acute ends, hyaline, uniseptate, deeply constricted at the septum, sometimes curved, smooth, with 4–6 prominent guttules, and gelatinous sheath. Asexual morph: Undetermined.


Habitat: Saprobic on decaying wood submerged in Dulong River and Nujiang River


Known distribution: China


Material examined: CHINA, Yunnan Province, saprobic on decaying wood submerged in Dulong River, May 2015, Z.L. Luo, S-428 (MFLU 18–1326, holotype; HKAS 92685, isotype), ex-type living culture (MFLUCC 16–0907); saprobic on decaying wood submerged in Dulong River, May 2015, Z.L. Luo, S-516, living culture MFLUCC 16–1297; saprobic on decaying wood submerged in Nujiang River, May 2015, Z.L. Luo, S-538 (MFLU 18–1327, paratype), ex-paratype living culture (DLUCC 0538).


GenBank numbers: LSU: MK106098, TEF1-a: MK109802 (MFLUCC 16–0907); LSU MK106099 (MFLUCC 16–1297); LSU: MK106100, SSU: MK106109, TEF1-a: MK109803 (DLUCC 0538).


Notes: The new species Amniculicola guttulata resembles A. aquatica in having superficial, coriaceous, globose to subglobose ascomata, cylindrical asci of similar size and with a short, narrowed, twisted, bifurcate pedicel, and fusiform, hyaline, uniseptate ascospores deeply constricted at the septum. However, A. guttulata differs from A. aquatica has a thinner peridium and smaller ascospores (23–27 × 5–7 vs. 24–32 × 6–8 µm). Phylogenetic analysis showed that isolates of A. guttulata formed a separate clade in this genus with high support (98 ML/0.99 Bayesian).