Phyllachorales » Phyllachoraceae » Tamsiniella

Tamsiniella labiosa

Tamsiniella labiosa NS.W. Wong, K.D. Hyde, W.H. Ho & S.J. Stanley, Can. J. Bot. 76(2): 334 (1998)

Facesoffungi number: FoF05052

 

Sexual morph: Ascomata 100–120 lm high, 130–150 µm  diam. [type: 130–225 µm high, 180–250 µm diam.], black, gregarious or scattered, superficial, subglobose to ellipsoidal, uni-loculate, thin-walled, laterally ostiolate, with a mass of spores oozing when old. Peridium 6–9 µm wide [type: 5–11 µm wide], comprising 3–4 layers of dark brown, thickwalled, compressed cells of textura angularis. Hamathecium

comprising numerous, ca 6 µm wide at the base, 2 µm diam. at the apex [type: 4–5 µm wide], cylindrical, unbranched, hyaline, septate, paraphyses, slightly constricted at the septa, tapering towards the apex. Asci 80–110 × 7.5–9 µm (= 93 × 8.5 µm, n = 15) [type: 80–102 × 8–10.5 µm], 8-spored, unitunicate, cylindrical to cylindric-clavate with a short, twisted pedicel, apically obtuse and inwardly concave, slightly wider than subapical apparatus, with J-, a refractive, lip-like apical ring, 1.9–2 × 2.9–3.1 µm. Ascospores 17–20 × 3.5–5 µm ( = 19 × 4.5 µm, n = 15) [type: (12–)15–21 × 3.8–4.5(–5) µm], overlapping 1-seriate, hyaline, aseptate, fusiform to ellipsoidal, straight or curved, guttulate, thin-walled, with a thin, hyaline, mucilaginous sheath, 1–2 lm wide. Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA reaching 20 mm in 12 days at 25 °C, circular, white to yellow–brown from above, white to light yellow from below, surface smooth with sparse mycelium, dry, edge entire.

 

Material examined: CHINA, Yunnan Province, Pingbian, on submerged wood in a small river, 20 September 2017, W. Dong, WF-33A (MFLU 181191, a reference specimen is designated here; ibid., KUN-HKAS 101711), living culture, MFLUCC 181018 = KUMCC 180060.

 

Known host and distribution: On submerged woods or twigs in Australia, Brazil, China, Hong Kong (Wong et al. 998; Barbosa et al. 2013; this study).

 

 

GenBank Accession No: ITS = MK034865, LSU =MK034866, SSU = MK034867.

 

Notes: Our isolate shares the size range of asci, ascospores, peridium and paraphyses with the type of Tamsiniella labiosa (see description). However, it has a slightly smaller ascomata compared to the type. Our isolate was collected from submerged wood in Yunnan, China, whereas the type specimen was collected from submerged wood in North Queensland, Australia. Based on morphological comparison and geographical distribution, we hence, designate our collection as a reference specimen of T. labiosa. In phylogenetic tree, Tamsiniella labiosa forms a distinct lineage in the order Phyllachorales M.E. Barr with moderate support (77% ML and 0.99 BYPP). Phyllachorales species are distinctive as they are biotrophic on various hosts (Pearce and Hyde 1994; Dayarathne

et al. 2017; Mardones et al. 2017, 2018). Phyllachorales species are characterized by deep black stromata of various shapes; pseudostroma inside the host tissue and usually beneath an epidermal clypeus; perithecia usually strongly melanized; cylindrical to clavate asci with an inconspicuous apical ring; and globose to filiform, mostly hyaline, 1-celled, rarely brown or septate ascospores (Parbery 1967; Cannon 1991; Dayarathne et al. 2017; Mardones et al. 2017, 2018). However, our collection was isolated from decaying wood submerged in freshwater, which was the same habitat as another freshwater ascomycete genus Ascovaginospora Fallah et al. in Phyllachoraceae (Wijayawardene et al. 2018a). Therefore, Tamsiniella is accommodated in Phyllachoraceae based on phylogenetic analyses.