Kirschsteiniotheliales » Kirschsteiniotheliaceae » Kirschsteiniothelia

Kirschsteiniothelia inthanonensis

Kirschsteiniothelia inthanonensis J. Louangphan & de Farias, in Gomes de Farias, Afshari, Hittanadurage Silva, Louangphan, Karimi & Boonmee, MycoKeys 101: 353 (2024)

Index Fungorum number: IF 901384; Facesoffungi number: FoF 14982

Saprobic on decaying wood. Sexual morph: Not observed. Asexual morph: Hyphomycetes. Colonies on the host substrate are superficial, effuse, long hairy, fascicular, scattered, dark brown to black. Mycelium superficial and immersed, composed of branched, septate, pale brown and smooth hyphae. Conidiophores 611–1549 × 2.5–6.6 μm (x̄ = 1070 × 4.1 μm, n = 20), macronematous, synnematous, compact fasciculate, straight to flexuous, brown to dark brown, branched at the apex, multi-septate, thick and smooth-walled. Conidiogenous cells 15–45 × 6.7–10.4 μm (x̄ = 24.3 × 8 μm, n = 20), monotretic to polytretic, calyciform, integrated, discrete, terminal, darkened at the apex, proliferating portion, brown, 2–4 septate. Conidia 24–230 × 5.7–14.3 μm (x̄ = 101 × 9 μm, n = 15), acrogenous, solitary, obclavate, rostrate, straight or curved, truncate at base, grey to brown, pale at apex, partly tapering towards and rounded at the apex, 2–10– euseptate, smooth-walled. 

Culture characteristics: Conidia germinated on PDA within 48 hours. Germ tubes germinated from end cell. Colony, reaching 30–35 mm diam. after one month at room temperature, circular form, flat, undulate edges, dense velvety surface, dark green on the surface, white mycelium on the tip, dark in reverse with dark green margin.

Material examined: Thailand, Chiang Mai, Chom Thong, Doi Inthanon National Park, on twigs of Quercus oleoides, 30 November 2022, Veenavee Silva, DIFWS5-01 (MFLU 23–0420, holotype), ex-type living culture MFLUCC 23–0277.

Notes: Kirschsteiniothelia inthanonensis (MFLUCC 23–0277) resembles K. septemseptatum and K. nabanheensis in having septate, cylindrical conidiophores with branches near apex, integrated, terminal conidiogenous cells and solitary, obclavate, septate conidia without mucilaginous sheaths. However, K. inthanonensis MFLUCC 23–0277 has longer and smaller conidiophores than K. septemseptatum and K. nabanheensis (611–1549 μm vs. 250–580 μm and 320–588 μm) and (2.5–6.6 μm vs. 6.5–14.5 μm and 8–12 µm), respectively and elongated conidia (Jayawardena et al. 2022; Liu et al. 2023). In addition, our phylogenetic analyses show that K. inthanonensis forms an independent branch with 100% MLBS and 1.00 BYPP support. BLASTn base pair comparisons between K. inthanonensis (MFLUCC 23–0277) and K. septemseptatum (MFLU 21–0126) show 95% similarity of ITS (479/504, 6 gaps), 99% similarity of LSU (844/853, no gaps) and 99% similarity of SSU (787/789, 2 gaps). Kirschsteiniothelia nabanheensis (HJAUP C2004) shows 94% similarity of ITS (483/513, 7 gaps), 99% similarity of LSU (540/547, no gaps) and 98% similarity of SSU (864/883, no gaps). Based on these data, we introduce K. inthanonensis as a new species.

 

Figure 1. Maximum Likelihood phylogenetic tree generated from ITS, LSU and SSU sequence data for selected Kirschsteiniotheliales and related Dothideomycetes orders. The tree is rooted with Stemphylium vesicarium (CBS 191.86 and MFLUCC 14–0920). Newly-generated sequences are in blue and new species are in bold. Holotype and ex-type strains are symbolic by “T”. Maximum Likelihood bootstrap (MLBS) values ≥ 70% and Bayesian posterior probabilities (BYPP) ≥ 0.95 are shown at the nodes.

 

Figure 2. Kirschsteiniothelia inthanonensis (MFLU 23–0420, holotype) a, b colonies on the host c, d conidiophores and conidia e regeneration of conidiophores f–j conidiogenous cells and conidia l–o conidia p germinating conidium q, r colony on PDA (front and reverse). Scale bars: 500 μm (b–d); 50 μm (e–j); 20 μm (k–p).

 

References

1. de Farias ARG, Afshari N, Silva VSH, Louangphan J, Karimi O, Boonmee S (2024) Three novel species and new records of Kirschsteiniothelia (Kirschsteiniotheliales) from northern Thailand. MycoKeys 101: 347–370. https://doi.org/10.3897/mycokeys.101.115286

 

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