Hysteriales » Roussoellaceae » Neoroussoella

Neoroussoella leucaenae

Neoroussoella leucaenae Jayasiri, E.B.G. Jones & K.D. Hyde, in Jayasiri, Hyde, Jones, McKenzie, Jeewon, Phillips, Bhat, Wanasinghe, Liu, Lu, Kang, Xu & Karunarathna, Mycosphere 10(1): 106 (2019) Index Fungorum number: -            Facesoffungi number: -


Sexual morph: Ascomata 130–195 μm high, 150–170 μm diam., visible as raised, aggregated, small, dark brown to black dots on the host surface, immersed, erumpent through host epidermis by minute papilla, scattered, solitary, sometimes gregarious, globose to subglobose, occasionally irregular in shape, uni-loculate, glabrous, ostiole central with minute papilla. Peridium (7–)10–18 μm, composed of several layers, of hyaline to dark brown pseudoparenchymatous cells, inner layer comprising flattened, hyaline to brown cells, arranged in a textura prismatica to textura angularis, outer layer comprising thick, dark brown to black cells, arranged in a textura angularis. Hamathecium composed of dense, 1–3 μm wide, hyaline, septate, filamentous, anastomosed pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci (35–)42–68(–75) × 4–5(–6) μm ( = 53 ×9 5.5, n = 15), 8-spored, bitunicate, cylindrical, with a short pedicel, apically rounded, with an indistinct ocular chamber. Ascospores 7–10(– 15) × 3–4.5(– 6.5) μm ( = 9.4 × 4.4, n = 40), overlapping 1-seriate, hyaline when young and medium to dark brown when mature, ellipsoidal to ovoid, with rounded ends, 1-septate, constricted at the septum, rough-walled, with two guttules, and longitudinal striations. Asexual morph: Undetermined.


Known hosts and distribution: On decaying pod carpel of Leucaena sp. and Pterocarpus sp., Hevea brasiliensis (Thailand).


Culture characteristics: Colonies on PDA reaching 15–25 mm diam. after 3 weeks at 25–30 ºC, circular, medium dense, edge entire, velvety; from above, light grey at the margin, white to light grey at the centre; from below, white at the margin, light to dark brown at the centre.


Material examined: THAILAND, Phayao Province, Muang District, Mae Ka, on dead twigs of Hevea

brasiliensis, 3 January 2017, C. Senwanna, RBPY028 (MFLU 17-1985), living culture, MFLUCC 17-0346.


GenBank Accession No: ITS: MH590694, LSU: MH590690, SSU: MH590692, TEF1-a: MH590688.


Notes: Neoroussoella leucaenae (MFLU 17-1985) is found on Para rubber in Thailand for the first time. Phylogenetically our strain (MFLUCC 17-0346) clusters with the type strain of N. leucaenae (MFLUCC 18-1544) and strain MFLUCC 17-0927 with moderate support (76% ML and 1.00 BYPP; Fig. 39). There is different only one and three nucleotide base positions in ITS and TEF1-a among N. leucaenae strains MFLUCC 18-1544, MFLUCC 17-0927 and MFLUCC 17-0346, which confirms that they are conspecific.


Figure X. Neoroussoella leucaenae (MFLU 17-1985). a–c Habit on substrate. d Section through ascoma. e Peridium. f Pseudoparapyses. g Asci.h Ascospores. i Germinated ascospores. Scale bars b–d = 200 μm, g = 30 μm, e = 20 μm, f, h, i = 10 μm



Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, et al. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity (2019) 95:1–273


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project entitled:

"The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants Rhododendron species and Dracaena species"

(Grant No. DBG6080013)

"Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub-region"

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    1 Center of Excellence in Fungal Research
  • Mae Fah Luang University Chiang Rai
    57100 Thailand
  • 2 Kunming Institute of Botany
  • Chinese Academy of Sciences,
  • Honghe County 654400, Yunnan, China

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