Gloniopsis calami
Gloniopsis calami Konta & K.D. Hyde, Fungal Diversity 80: 34 (2016)
Facesoffungi number: FoF02366
Saprobic on dead wood. Sexual morph: Ascomata 174–229 µm high × 136.5–209 µm diameter (x̄ = 196.5 × 162 µm), hysterothecial, erumpent to entirely superficial, solitary, or gregarious, oval or ellipsoid to greatly elongate, straight to curved, scattered, dark, carbonaceous, brittle, with a sunken longitudinal slit-like opening. Peridium 27–40.5 μm wide, composing thick layers of dark brown cells of carbonaceous texture, inner layers composed of several layers of hyaline to brown cells of textura angularis. Hamathecium comprising 1.5–2 μm thin, cylindrical, hyaline, branched, septate, anastomosing pseudoparaphyses. Asci 53–78 × 13–19 μm (x̄ = 64 × 16 µm, n = 10), 8-spored, bitunicate, fissitunicate, saccate, broadly clavate to cylindrical, slightly curved, short pedicellate, with knob-like pedicel, apically rounded, with a well-developed ocular chamber. Ascospores 15–20 × 7–9 μm (x̄ = 18 × 8 μm, n = 20), 2-seriate, overlapping, reddish-brown to brown, dictyosporous, fusiform, oblong or ellipsoidal to cylindrical, 4–6-trans-septate and with 2–4 vertical septa, constricted at the septa, smooth-walled. Asexual morph: Undetermined.
Culture characteristics: Ascospores germinating on WA within 12 h and germ tubes produced from spore cells. Colonies on MEA 18 mm after 7 days at 28 °C. Mycelium superficial, low convex, slightly effuse hairy, edge entire, dark brown. Aerial mycelium, radiating outwards, superficial, septate, hyaline to brown, smooth-walled.
Known distribution (based on molecular data): Thailand (Phang-Nga Province) – Hyde et al. (2016), Chiang Rai Province (this study).
Known substrates (based on molecular data): On dead Calamus sp. (Arecaceae) Hyde et al. (2016), on dead wood of unidentified host (this study).
Material examined: Thailand, Chiang Rai, Huai Kang Pla, on dead wood of unidentified host, 25 October 2010, S. Boonmee, HPK02 (MFLU 10–0973, new geographical record); living culture (MFLUCC 10–0927 = BCC 52145).
GenBank Accession No: ITS: MN608546, LSU: MN577415, SSU: MN577426.
Notes: Gloniopsis calami was introduced by Hyde et al. (2016) based on morphology and phylogenetic analysis. This species was described from a palm from southern Thailand. Our strain shares similar characteristics such as ascomata, asci and ascospores with Gloniopsis calami (MFLUCC 15–0739). Further, phylogenetic analysis based on LSU and SSU data indicated that our strain clusters with an asexual species, Gloniopsis leucaenae (C289) with poor support (Fig. 3). 35 Comparison of ITS regions of Gloniopsis leucaenae (C289) and G. calami MFLUCC 10–0927 reveals 6 base pair (0.92%) differences. However, base pair comparison of ITS, LSU and SSU of Gloniopsis calami MFLUCC 15–0739 and G. calami MFLUCC 10–0927 are 100% similar (data not shown here). Therefore, we identify our strain as Gloniopsis calami and this is the first report of G. calami from dead wood in northern Thailand.
Figure X. Gloniopsis calami (MFLU 10–0973, new geographical record). a, b Material and Appearance of ascomata on woody substrate. c Section of ascoma. d Peridium. E Pseudoparaphyses. f–i Asci. j–n Ascospores. Scale bars: c = 50 µm, d, f–i = 20 µm, e, j–n = 10 µm.
Gloniopsis calami Konta. & K.D. Hyde, Fungal Divers 81: 34 (2016)
Facesoffungi number: FoF02366
Saprobic on decaying wood submerged in freshwater habitats. Sexual morph: Hysterothecia 130–200 μm high, 150–180 μm diameter, erumpent to superficial, solitary to gregarious, scattered, dark, straight to flexuous. Peridium 25–50 μm thick, carbonaceous, thick-walled, relatively smooth on the outer surface. Hamathecium comprising dense, branched, hyaline, septate pseudoparaphyses. Asci 107–127 × 17–21 µm (x̄ = 117 × 19 µm, n = 10), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate, apically rounded, with a well-developed ocular chamber. Ascospores 23–27 × 10–12 µm (x̄ = 25 × 11 µm, n = 20), overlapping 1–2-seriate, pale brown to dark brown, dictyosporous, fusiform, slightly curved to straight, 3–5-trans-septate and with 1–4 vertical septa, constricted at the septa, smooth-walled. Asexual morph: Undetermined.
Culture characteristics: Ascospores germinating on PDA within 14 h and germ tubes produced from spore cells. Colonies on MEA reached 20 mm after 10 days at 25 °C. Mycelium superficial, low convex, slightly effuse hairy, edge entire, dark brown.
Known distribution (based on molecular data): Thailand, Phang-Nga Province (Hyde et al. 2016), Chiang Rai Province (this study).
Known hosts (based on molecular data): On dead Calamus sp. (Arecaceae) (Hyde et al. 2016), on submerged dead wood of unidentified host (this study).
Material examined: Thailand, Chiang Mai Province, saprobic on decaying wood submerged in a freshwater pond, January 2014, Z.L. Luo, ZL-27 (MFLU 15–0074, new to freshwater habitats), living culture MFLUCC 14–0049.
GenBank Accession No: ITS: MN 860550, LSU: MN 860555.
Notes: Our strain (MFLUCC 14–0049) shares similar characteristics such as ascomata, asci and ascospores with Gloniopsis calami (MFLUCC 15–0739). Further, phylogenetic analysis based on LSU and SSU data indicated that our strain clusters with Gloniopsis calami with high statistical support (98% ML) (Fig. 3). This is the first report of G. calami from submerged dead wood in freshwater habitats.
Figure X. Gloniopsis calami (MFLUCC 10–0927, living culture). a Germinating spore. b, c Culture colonies on MEA from surface and reverse. d–f Aerial hyphae in culture. Scale bars: a, d–f = 10 µm.
Reference: Hyde KD, de Silva NI, Jeewon R, Bhat DJ et al. (2020) AJOM new records and collections of fungi: 1-100. Asian Journal of Mycology 3, 22-294.
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