Hypocreales » Nectriaceae » Volutella

Volutella thailandensis

Volutella thailandensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [110] (2018)

Index Fungorum number: IF554540         Facesoffungi number: FoF04548

Sexual morph: Ascomata 115140 × 100130 μm (= 128 × 114 μm, n = 5), scattered to gregarious, superficial, flat at the base, conspicuous on host surface, easy to remove, black, dull, solitary, uniloculate, black with conspicuous setae, without ostiole. Peridium composed of several layers of thin-walled subhyaline to pale brown cells of textura prismatica. Hamathecium composed of 29.4 μm wide, cylindrical, cellular, unbranched, guttulate, septate paraphyses. Asci 2540 × 69 μm ( = 36 × 7.4 μm, n = 20), (6)8-spored, unitunicate, cylindrical to cylindrical-clavate, with short furcate pedicel. Ascospores 1016 × 25 μm (= 14 × 3 μm, n = 20), fusiform, curved towards both ends, 1-septate, constricted at septum, hyaline to subhyaline, guttulate, with mucilaginous sheath. Asexual morph: Hyphomycetous. Conidiophores aggregated into sporodochia or synnemata, without hyaline setae around the margin of conidiomata. Conidiogenous cells 715 × 12.5 μm (= 12 × 2 μm, n = 20), monophialidic, hyaline, subulate. Conidia 38 × 13 μm (= 5 × 2 μm, n = 40), slimy, aseptate, hyaline, oblong, guttulate, without a mucilaginous sheath.

Culture characteristics: Ascospores germinating on MEA within 12 h. Colonies on MEA, filamentous, hyaline, smooth and flat on surface media.

Habitat: Known to inhabit saprobic on dead leaves of Pandanus sp.

Known distribution: Thailand.

Material examined: Thailand, Prachuap Khiri Khan Province, Bang Saphan District, on Pandanus sp., 30 July 2015, S. Tibpromma SF15-034 (MFLU 18-0014, holotype; HKAS 100839, isotype); ex-type living culture, MFLUCC 16-0366 = MFLUCC 16-0368 = KUMCC 16-0160.

GenBank Accession No: LSU: MH376742; ITS: MH388368; SSU: MH388332; TEF1: MH388402.


Notes: The sexual and asexual morphs of Volutella thailandensis fits well within the holomorphconcept for Volutella (Grafenhan et al. 2011; Seifert et al. 2011). In the phylogenetic tree based on the sequences of multi-gene, V. thailandensis clusters with V. krabiense, but there are more than 30 bp (5.83%) differences in 514 ITS (+5.8S) nucleotides between the two species and thus indicating they are distinct species. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 16-0366 is Volutella sp. with 99% identity to the strain DoF21 (JQ388266).

Figure X. Volutella thailandensis (MFLU 18-0014, holotype). a Appearance of ascomata on host substrate. b Ascomata. c Ascoma wall. d Hamathecium. e Asci. f Asci stained with cotton blue reagent. g Papilla. h, i Ascospores stained with cotton blue reagent. j, k Culture growing on MEA media. l Sporodochia formed in culture. m Conidiogenous cells and conidia formed in culture. n Conidia formed in culture. Scale bars: b = 50 μm, c, e, f, m, n = 5 μm, d, h, i = 2 μm, g = 10 μm, l = 200 μm.


Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, Phillips AJL, Wanasinghe DN, Samarakoon MC, Jayawardena RS, Dissanayake AJ, Tennakoon DS, Doilom M, Phookamsak R, Tang AMC, Xu JC, Mortimer PE, Promputtha I, Maharachchikumbura SSN, Khan S, Karunarathna SC (2018) Fungal diversity notes 840928: micro-fungi associated with Pandanaceae. Fungal Diversity. 93:1-160.


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project entitled:

"The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants Rhododendron species and Dracaena species"

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"Impact of climate change on fungal diversity and biogeography in the Greater Mekong Sub-region"

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