Hypocreales » Nectriaceae » Cylindrocladiella

Cylindrocladiella xishuangbannaensis

Cylindrocladiella xishuangbannaensis Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [105] (2018)

Index Fungorum number: IF554536         Facesoffungi number: FoF04546

 

Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Conidiophores dimorphic, penicillate and subverticillate, mononematous, hyaline, penicillate, 456–476 × 6–7 μm (x̄ = 469 × 6.4 μm, n = 5), conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth; stipe extension aseptate, straight, thick-walled with one basal septum, terminating in a thin-walled, cylindrical vesicle. Penicillate conidiogenous apparatus 18–47.5 × 2.5–4 μm (x̄ = 30 × 3.6 μm, n = 10), with primary branches aseptate, secondary branches aseptate, each terminal branch producing 2–4 phialides; phialides doliiform to cymbiform, hyaline, aseptate, apex with minute periclinal thickening and collarette. Subverticillate 6–12 × 2–4 μm (x̄ = 9 × 3 μm, n = 20), conidiophores in moderate numbers, comprising a septate stipe, primary and secondary branches terminating in 1–3 phialides; primary branches straight, hyaline, 0–1-septate. Conidia 52.5–60.5 × 3–6 μm (x̄ = 57 × 5 μm, n = 40), cylindrical, rounded at both ends, straight, 1-septate, frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

 

Culture characteristics: Conidia germinating on PDA within 12 h. Colonies on PDA, circular, entire edge with cinnamon, raised on surface media, produce pigment on the media.

 

Habitat: Known to inhabit bark saprobic on dead leaves of Pandanus sp.

 

Known distribution: China.

Material examined: China, Yunnan Province, Xishuangbanna, Nabanhe, on Pandanus sp., 28 July 2016, S. Tibpromma NBH09 (HKAS 96209, holotype); ex-type living culture, KUMCC 16-0146 = MFLUCC 17-0559.

 

GenBank Accession No: LSU: MH376709; ITS: MH388337; SSU: MH388306; RPB2: MH412727.

 

Notes: This species morphologically fits within the generic concepts of Cylindrocladiella. Based on the multigene sequence analyses, C. xishuangbannaensis clusters with C. camelliae (Venkataram. & C.S.V. Ram) Boesew. We compare the morphology with C. camelliae, but they differ. Cylindrocladiella camelliae has a slight difference in terminal vesicle shape with conidia size 15–26 9 2–3.5 lm (Boesewinkel 1982). In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of KUMCC 16-0146 is C. solicola with 99% identity to the strain CMW47198 (MH017021), while the closest matches with the RPB2 sequence were with 99% identical C. camelliae strain CPC 234 (KM232304).

Figure X. Cylindrocladiella xishuangbannaensis (HKAS 96209, holotype). a Colonies on dead leaves of Pandanus sp. b Penicillate conidiophores. c, d Conidiophore branches, conidiogenous apparatus and phialides. e–h Conidia. i, j Colony on PDA from above and below. Scale bars: b = 100 lm, c–h = 20 lm.

 

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, Phillips AJL, Wanasinghe DN, Samarakoon MC, Jayawardena RS, Dissanayake AJ, Tennakoon DS, Doilom M, Phookamsak R, Tang AMC, Xu JC, Mortimer PE, Promputtha I, Maharachchikumbura SSN, Khan S, Karunarathna SC (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity. 93:1-160.

 

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