Hypocreales » Cordycipitaceae » Cordyceps

Cordyceps pruinosa

Cordyceps pruinosa Petch, Trans. Br. mycol. Soc. 10(1-2): 38 (1924)

Index Fungorum number: IF165068         Facesoffungi number: FoF07468

Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Stroma emerging from host, single, erect, cylindrical, apically branched, red, pale yellow at the base, fleshy, with white straight hyphae and interlaced vegetative hyphae mass occurring on the surface of upper stroma. In vitro, Synnemata orange, erect, compacted below, apically branched, becoming finger-like. Hyphae 0.6–1.20 (x̄ = 1, n = 20) μm, aseptate, hyaline, smooth-walled. Conidiophores macronematous, prostrate, branched, bearing solitary or 2–3 whorls of phialides. Conidiogenous cells phialidic. Phialides 11–30 × 1.2–1.6 (x̄ = 16 × 1.3, n = 20) μm, aseptate, monophialidic, gradually tapering towards the apex, hyaline, straight. Conidia 1.8–4.8 × 0.9–2.7 (x̄ = 3 × 1.5, n = 30) μm, globose, oval, cylindrical, aseptate, smooth-walled, hyaline, aggregating in imbricated slimy chains at the tip of phialides.

 

Culture characteristics: slow-growing on PDA, reaching 4 cm in diameter after incubating at room temperature for 26 days, yellow from upper and reverse view, floccose, dense, circular, feathery margin, with several erect, orange synnemata developing at central regions, without diffused pigment. The sporulation occurs on upper part of synnemata.

Material examined: China, Yunnan Province, Kunming City, Western hill Park. On Limacodid pupa, 27 July 2018, Deping Wei (HKAS 102537, new sequence data), living culture KUMCC 18–0340.

Known hosts: Cocoon of a Limacodidae insect (Meng et al. 2014, Zha et al. 2019); Iragoides fasciata (Lepidoptera) (Spatafora et al. 2007, Sung et al. 2007).

Known distribution: Korea (Bae et al. 2002, Moon et al. 2018); Guangdong and Guizhou in China (Wang et al. 2008Meng et al. 2014, Zha et al. 2019); Japan (Nikoh & Fukatsu 2001, Kepler et al. 2012).

GenBank Accession No: ITS: MT012347, LSU: MT012354, SSU: MT012361, tef 11:MT025053.

Notes: In the phylogenetic tree, our collection XS2703 is closest to Cordyceps pruinosa (ARSEF 5413) and C. ninchuckispora with moderate support (81% MP/ 0.99 PP). The strain ARSEF 5413 is not linked to any description (Kepler et al. 2017). Cordyceps pruinosa was initially described by Petch (1924) with its asexual morph linked to Mariannaea pruinosa by Liang et al. (1991) but not confirmed with molecular data. The asexual morph of Cordyceps pruinosa was characterized by verticillate conidiophores and ovoid, ellipsoidal, cylindrical, rarely septate, hyaline conidia, which adhere in imbricate chains (Liang et al. 1991). Our collection is consistent with the concept of Cordyceps pruinosa in the shape and size, as well as the arrangement of conidia. A comparison of tef1 sequences between our isolates and two strains of Cordyceps ninchuckispora (EFCC 5197 and EFCC 5693) shows 17/862 bp (2%) differences, but identical to Cordyceps pruinosa (ARSEF 5413) within 862bp. In ITS, our collection is identical to Cordyceps pruinosa (ARSEF 5413) within 355 bp. We report a new collection of Cordyceps pruinosa according to the guidelines of Jeewon & Hyde (2016).