Hypocreales » Bionectriaceae » Lasionectria

Lasionectria krabiense

Lasionectria krabiense Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [95] (2018)

Index Fungorum number: IF554528         Facesoffungi number: FoF04540

 

Sexual morph: Ascomata 140–200 × 220–260 μm (x̄ = 168 × 236 μm, n = 5), solitary, superficial, globose to subglobose, with apex flattened with a minute papilla, orange to brownish orange, collapsing and becoming cupulate when dry, without ostiole. Peridium 15–30 μm wide, composed of hyaline to orange, hypha-like cells of textura prismatica. Hamathecium 1.5–3 μm wide, comprising numerous, dense, filiform, filamentous, branched, guttulate, septate paraphyses. Asci 30–55 9 8–11 μm (x̄ = 43 × 9 μm, n = 20), 6–8-spored, unitunicate, cylindrical to cylindricclavate, short-pedicellate, apically rounded. Ascospores 15–20 × 3–5 μm (x̄ = 17 × 4 μm, n = 20), overlapping bi-seriate, fusoid-ellipsoidal, hyaline to subhyaline, 1-septate in the middle, rough, with longitudinal striations, granulate, not surrounded by a mucilaginous sheath. Asexual morph: Undetermined.

 

Culture characteristics: Ascospore germinating on MEA within 12 h. Colonies on MEA, circular to irregular, undulate with white to yellow–white, flat on media, rough with wrinkles edge.

Habitat: Saprobic on dead leaves of Pandanus sp..

 

Known distribution: Thailand and China.

 

Material examined: Thailand, Krabi Province, Mueang Krabi District, on Pandanus sp., 14 December 2014, S. Tibpromma SF14-022 (MFLU 16-0540 holotype; HKAS 100829, isotype); living culture, MFLUCC 15-0673; CHINA, Yunnan Province, Xishuangbanna, Nabanhe, on Pandanus sp., 27 July 2016, PE Mortimer NBH13 (HKAS 96213, paratype).

GenBank Accession No: LSU: MH376725; ITS: MH388352; SSU: MH388319; TEF1: MH388387.

Notes: On combined gene analysis, Lasionectria krabiense clusters with L. antillana (Lechat & Courtec.) Schroers, Ashrafi & W. Maier with strong bootstrap support (100% in ML). The ascomata and ascospore morphology of both species is very similar, but L. antillana has 12.7 × 3.2 µm ascospores without longitudinal striations (Lechat and Courtecuisse 2010). There are more than 34 bp (6.77%) differences in the 502 ITS (+5.8S) nucleotides of L. antillana and L. krabiense which indicates they are distinct species. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 15-0673 is L. antillana with 94% identity to the strain CBS 122797 (KY607537).

Figure X. Lasionectria krabiense (MFLU 16-0540 holotype). a Colonies on dead leaf of Pandanus sp. b Section of ascoma. c Papilla. d Paraphyses. e Peridium. f, g Asci. h–k Ascospores. l Germinating ascospore. m, n Colony on MEA from above and below. Scale bars: a = 200 lm, b, f = 20 μm, c–e = 5 μm, g = 10 μm, h–l = 2 μm.

Reference: Tibpromma S, Hyde KD, McKenzie EHC, Bhat DJ, Phillips AJL, Wanasinghe DN, Samarakoon MC, Jayawardena RS, Dissanayake AJ, Tennakoon DS, Doilom M, Phookamsak R, Tang AMC, Xu JC, Mortimer PE, Promputtha I, Maharachchikumbura SSN, Khan S, Karunarathna SC (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity. 93:1-160.

 

 

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