Hypocreales » Bionectriaceae » Lasionectria

Lasionectria krabiense

Lasionectria krabiense Tibpromma & K.D. Hyde, in Tibpromma et al., Fungal Diversity: 10.1007/s13225-018-0408-6, [95] (2018)

Index Fungorum number: IF554528         Facesoffungi number: FoF04540

 

Sexual morph: Ascomata 140–200 × 220–260 μm (= 168 × 236 μm, n = 5), solitary, superficial, globose to subglobose, with apex flattened with a minute papilla, orange to brownish orange, collapsing and becoming cupulate when dry, without ostiole. Peridium 15–30 μm wide, composed of hyaline to orange, hypha-like cells of textura prismatica. Hamathecium 1.5–3 μm wide, comprising numerous, dense, filiform, filamentous, branched, guttulate, septate paraphyses. Asci 30–55 9 8–11 μm (= 43 × 9 μm, n = 20), 6–8-spored, unitunicate, cylindrical to cylindricclavate, short-pedicellate, apically rounded. Ascospores 15–20 × 3–5 μm (= 17 × 4 μm, n = 20), overlapping bi-seriate, fusoid-ellipsoidal, hyaline to subhyaline, 1-septate in the middle, rough, with longitudinal striations, granulate, not surrounded by a mucilaginous sheath. Asexual morph: Undetermined.

 

Culture characteristics: Ascospore germinating on MEA within 12 h. Colonies on MEA, circular to irregular, undulate with white to yellow–white, flat on media, rough with wrinkles edge.

Habitat: Saprobic on dead leaves of Pandanus sp..

 

Known distribution: Thailand and China.

 

Material examined: Thailand, Krabi Province, Mueang Krabi District, on Pandanus sp., 14 December 2014, S. Tibpromma SF14-022 (MFLU 16-0540 holotype; HKAS 100829, isotype); living culture, MFLUCC 15-0673; CHINA, Yunnan Province, Xishuangbanna, Nabanhe, on Pandanus sp., 27 July 2016, PE Mortimer NBH13 (HKAS 96213, paratype).

GenBank Accession No: LSU: MH376725; ITS: MH388352; SSU: MH388319; TEF1: MH388387.

Notes: On combined gene analysis, Lasionectria krabiense clusters with L. antillana (Lechat & Courtec.) Schroers, Ashrafi & W. Maier with strong bootstrap support (100% in ML). The ascomata and ascospore morphology of both species is very similar, but L. antillana has 12.7 × 3.2 µm ascospores without longitudinal striations (Lechat and Courtecuisse 2010). There are more than 34 bp (6.77%) differences in the 502 ITS (+5.8S) nucleotides of L. antillana and L. krabiense which indicates they are distinct species. In a BLASTn search on NCBI GenBank, the closest matches of ITS sequence of MFLUCC 15-0673 is L. antillana with 94% identity to the strain CBS 122797 (KY607537).