Hypocreales » Bionectriaceae » Clonostachys

Clonostachys eriocamporesiana

Clonostachys eriocamporesiana R.H. Perera & K.D. Hyde, in Hyde et al., Fungal Diversity 100: 197 (2020)

Index Fungorum number: IF556896         Facesoffungi number: FoF 06963

 

Hyperparasitic on ascomata of botryosphaeriaceous fungus, occurring on dead stems of Chromolaena odorata. Sexual morph: Stromata well-developed, erumpent. Ascomata 160–260 × 160–285 μm (x̄ = 200 × 218 μm), perithecial, solitary or densely crowded in groups, globose to subglobose, not collapsing or sometimes slightly laterally pinched when dry, light orange, KOH-, slightly papillate, surface smooth to slightly roughened, but not distinctly warted. Ostiole periphysate. Peridium 32–40 μm wide, two regions: outer region 21–30 μm wide, comprising unevenly thickened hyaline to brownish yellow cells of textura globosa, becoming larger towards the periphery, most cells containing a vacuole; inner region 10–16 μm wide, consists with, flat, hyaline cells of textura angularis. Catenophyses up to 9.5 wide, septate, hyaline. Asci 58–66 × 7.3–10.3 μm (x̄ = 61.4 × 8.8 μm, n = 20), 8-spored, unitunicate, narrowly clavate, apex truncate with somewhat rounded edges, short stalked, apical ring J-. Ascospores 10.5–15.2 × 3.6–4.4 μm (x̄ = 12.5 × 4 μm, n = 30), 2-seriate to 1-seriate, median or submedian, 1-septate, equilateral or inequilateral, not constricted at the septum, ellipsoidal, 2–4-guttulate, hyaline, spinulose when mature. Asexual morph: Undetermined.

 

Culture characteristics: Ascospores germinating on PDA within 12 h. Colonies growing on PDA, reaching up to 26 cm in 7 days at 28 °C, surface effused, smooth, margin entire to undulate, aerial mycelium abundant, white, reverse white, yellowish at the centre.

 

Host and habitat: On on dead stems of Chromolaena odorata (Hyde et al. 2020)

 

Known host and distribution: Thailand

 

Material examined: THAILAND, Phayao Province, Phachang Noi Pong District, hyperparasite on ascomata of botryosphaeriaceous fungus on dead stems of Chromolaena odorata (L.) R.M. King & H. Rob. (Asteraceae), 9 November 2017, R.H. Perera, Bion 21 (MFLU 18-2713, holotype), ex-type living culture MFLUCC 17-2620.

 

GenBank Accession No: ITS: MN699132, TEF1-α: MN699964, TUB2: MN699965.

 

Notes: The new isolate clusters with ex-type of Clonostachys wenpingii (J. Luo & W.Y. Zhuang) Z.Q. Zeng & W.Y. Zhuang (HMAS 172156) and C. byssicola Schroers (CBS 364.78) with low statistical support. However, C. eriocamporesiana differs from C. byssicola in having smaller asci (58–66 versus 68–80 μm) and ascospores (10.5–15.2 × 3.6–4.4 versus 16.8–18.2 × 4.8–5.4 μm) (Schroers 2001). Clonostachys eriocamporesiana differs from C. wenpingii in having light orange ascomata versus yellow ascomata and larger asci (58–66 × 7.3–10.3 versus 33–44 × 5.5–8.0 μm) (Luo and Zhuang 2007). A comparison of the ITS and TUB2 nucleotides of C. eriocamporesiana and C. byssicola (CBS 364.78) shows 6/480 bp (1%) and 18/569 bp (3.1%) nucleotide differences. Another comparison of the ITS and TUB2 nucleotides of C. eriocamporesiana and C. wenpingii (HMAS 172156) shows 6/480 bp (1%) and 22/574 bp (3.8%) nucleotide differences. ITS and TUB2 nucleotide differences and significant morphological differences reveals that C. eriocamporesiana is a distinct taxon (Jeewon and Hyde 2016).

 

 

 

Figure X. Clonostachys eriocamporesiana (MFLU 18-2713, holotype). a Herbarium material. b, c Ascomata on host. d Section of ascoma. e Section of ostiole. f Section of peridium. g Catenophysis..h–k Asci. l–p Ascospores. q Germinating ascospore. r, s Colony on PDA. Scale bars: b, c = 200 µm, d = 100 µm, e, f = 50 µm, g– k = 20 µm, l–q = 10 µm.

 

References:

 

 

Hyde KD, Dong Y, Phookamsak R, Jeewon R, Bhat DJ, Jones EB, Liu NG, Abeywickrama PD, Mapook A, Wei D, Perera RH (2020) Fungal diversity notes 1151–1276: taxonomic and phylogenetic contributions on genera and species of fungal taxa.

Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambi- guities. Mycosphere 7:1669–1677

Luo J, Zhuang WY (2007) A new species and two new Chinese records of Bionectria (Bionectriaceae, Hypocreales). Mycotaxon 101:315–323

Schroers HJ (2001) A monograph of Bionectria (Ascomycota, Hypocreales, Bionectriaceae) and its Clonostachys anamorphs. Stud Mycol 46:1–214.

 

 

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