Glomerellales » Malaysiascaceae » Malaysiasca

Malaysiasca phaii

Malaysiasca phaii Crous & M.J. Wingf., in Crous et al., Persoonia 36: 373 (2016)

MycoBank MB817045

 

Sexual morph: Ascomata perithecial, base immersed in pine needles, to 250 µm wide, 400 µm tall, obpyriform, papillate, dark brown with setae around base of perithecium, pale brown; wall of 35 layers of pale brown textura prismatica. Paraphyses persistent, hyaline, branched, septate, longer than asci, 2.54 µm diam. Asci 580×912 µm, unitunicate, cylindrical-clavate, short-stipitate, apex truncate with shallow annulus, 8-spored. Ascospores biseriate in ascus, ellipsoid to oblong, apiculate at ends, with one end having minute mucoid cap, hyaline, smooth, frequently with large central guttule, becoming 1-septate and pale brown after discharge, (22)2427(30) × (6)7(8) µm. Asexual morph: Stroma brown, globose, erumpent, to 150 µm diam, giving rise to a fascicle of 210 conidiophores. Conidiophores subcylindrical, unbranched, erect, flexuous, basal cell slightly swollen, 712 µm diam; thick-walled, finely roughened, granular to slightly guttulate, 58 septate, grey-brown, becoming somewhat pale brown towards slightly tapered apex, rejuvenating percurrently, 150220 × 57 µm, with apical conidiogenous cell. Conidiogenous cells integrated, pale brown, subcylindrical, 5580 × 6 µm, phialidic, with periclinal thickening and minute collarette, apex 45 µm diam. Conidia solitary, aggregating in a slimy mass, ellipsoidal to cylindrical-ellipsoid to somewhat clavate, prominently guttulate, frequently with large central guttule, apex obtuse, base truncate, scar slightly thickened and darkened, frequently excentric, 34 µm diam, (16)1820(24) × (8)910(11) µm.

 

Culture characteristics: Colonies reaching up to 25 mm diam after 2 wk at 25 °C, with spreading, flat surface; margins feathery, lobate, and sparse aerial mycelium. On MEA surface and reverse pale luteous. On OAsurface dark mouse-grey. On PDA surface and reverse mouse-grey.

 

Habitat: Known to inhabit on leaves of Phaius reflexipetalus.

 

Known distribution: Malaysia.

 

Material examined: Malaysia, Sabah, Tawau, on leaves of Phaius reflexipetalus (Orchidaceae), 30 May 2015, M.J. Wingfield (holotype CBS H-22616, culture extype CPC 27548 = CBS 141321.

 

GenBank Accession No: ITS: KX228280, LSU: KX228331, act: KX228359

 

Notes: The LSU sequence of Malaysiasca phaii is 98 % (784/797) similarto that of Monilochaetes dimorphospora (MUCL 40959; GenBank HQ609480), 98 % (783/797) to Monilochaetes guadalcanalensis (CBS 346.76; GenBank GU180640) and 98 % (782/797) to Australiasca queenslandica (BRIP 24334c; GenBank HM237323). Morphologically, Malaysiasca resembles the genus Australiasca (Reblova et al. 2011), but is distinct in that it forms a stroma that gives rise to fascicles of conidiophores, whereas stromata are absent in Australiasca. In general the asexual morph is similar to species of Monilochaetes, and would be difficult to distinguish without the aid of molecular data.