Melomastia sichuanensis
Melomastia sichuanensis W.L. Li, Maharachch. & Jian K. Liu, Journal of Fungi 8 (1, no. 76): 12 (2022)
Index Fungorum number: IF841501; Facesoffungi number: FoF10535
Saprobic on dead woody twigs of Millettia leptobotrya. Sexual morph: Ascomata 260–500 μm high × 200–400 μm diam., (x̅ = 390 × 300 μm, n = 5), immersed to semi immersed, solitary or scattered, subglobose to obpyriform, coriaceous, black, with a central ostiole. Ostioles 110–170 × 100–160 μm (x̅ = 140 × 120 μm, n = 5), carbonaceous, black, papillate. Peridium 12–20 μm wide, 3–4 layered, composed of light brown to brown cells of textura angularis. Hamathecium 1.5–3 μm wide, comprising numerous filiform, unbranched, septate, hyaline, cellular pseudoparaphyses. Asci 125–145 × 6.3–7.2 μm (x̅ = 135 × 7 μm, n = 20), 8 spored, bitunicate, long cylindrical, straight or curved, short pedicellate, apically round with a small ocular chamber. Ascospores 15.5–18.4 × 4.7–5.7 μm (x̅ = 17 × 5 μm, n = 30), uniseriate, broad fusiform with rounded ends, hyaline, 2–3 septate (mostly 3 septate), constricted at the septa, guttules, thick and smooth walled. Asexual morph: Undetermined.
Culture characteristics: Ascospores germinating on PDA within 24 h at room temperature (25 °C). Germ tubes produced from the apical cell of an ascospore. Colonies on PDA, reaching 50 mm diameter after two weeks at 20–25 ℃, mycelia superficial, embedded in the medium, circular, fimbriate, umbonate, surface rough, granular, yellowish brown at the edge and white at the center, reverse white at edge, dark brown at center, pale yellow between the edge and center.
Material examined: China, Yunnan Province, Xishuangbanna, on dead woody twigs of Millettia leptobotrya (Fabaceae), 23 September 2019, G.C. Ren, MY10 (HKAS 122762), living culture KUMCC 21 0628.
Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, tef1-α, and rpb2 sequence data representing Pleurotremataceae. Related sequences are obtained following Li et al. (2022). Forty-six strains are included in the combined analyses, which comprise 3948 characters for SSU, LSU, ITS, tef1-α, and rpb2 alignment. Anisomeridium phaeospermum (MPN539) and A. ubianum (MPN94) were used as the outgroup taxa. The best-scoring RAxML tree with a final likelihood value of -28755.342067 is presented. The matrix had 2026 distinct alignment patterns, with 32.59% of undetermined characters or gaps. Estimated base frequencies were as follows; A = 0.238390, C = 0.263023, G = 0.288210, T = 0.210377; substitution rates AC = 1.022596, AG = 2.177198, AT = 1.179336, CG = 1.031321, CT = 5.495584, GT = 1.0000. The tree topology of the ML analysis is similar to the Bayesian analysis. Bootstrap values for ML equal to or greater than 70% and BYPP values greater than 0.95 (the rounding of values to 2 decimal proportions) are labelled on the nodes. Strains of the newly described species are in blue, while type strains are in bold.
Figure 2. Melomastia sichuanensis (HKAS 122762). a Material examined. b Appearance of ascomata on the host substrate. c Section of an ascoma. d Peridium. e Pseudoparaphyses. f–j Asci. k–p Ascospores. q, r Culture characters on PDA (q = from above, r = from below). Scale bars: c = 200 μm, d, e = 20 μm, f–j = 30 μm, k–p = 10 μm, q, r = 20 mm.
References
- Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8
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