Dothideales » Dothideaceae » Stylodothis

Stylodothis puccinioides

Stylodothis puccinioides (DC.) Arx & E. Müll., Stud. Mycol. 9: 11 (1975)

Index Fungorum number: IF324309; Facesofungi number: FoF00092

Saprobic on dead woody twigs of Cinnamomum glanuliferum. Sexual morph: Ascostromata 400–500 μm high × 600–1100 μm diam., (x̅ = 445 × 890 μm, n = 5), black, formed on erumpent basal stroma, solitary, thick at the base of the ascostromata, coriaceous, multiloculate, with 3–8 locules, cells of ascostromata composed of several layers of dark brown cells of textura angularis. Locules 90–160 μm high × 70–190 μm diam., (x̅ = 135 × 140 μm, n = 10), globose to subglobose, without an ostiole. Peridium 8–15 μm thick, one layered, comprising brown to dark brown cells of textura angularis. Hamathecium 1–2 μm wide, comprising cylindrical, septate pseudoparaphyses embedded in a hyaline, gelatinous matrix. Asci 70–90 × 10–13 μm (x̅ = 75 × 12 μm, n = 20), bitunicate, 4-spored, cylindrical-clavate, apically rounded, with short and rounded pedicellate. Ascospores 18–23 × 6.5–9 μm (x̅ = 20 × 7.7 μm, n = 30), uniseriate, slightly overlapping, ellipsoidal, brown, 1-septate, constricted at the central septum, upper cell wider than lower cell, thick and smooth-walled, without a gelatinous sheath. Asexual morph: Undetermined.

 

Culture characteristics: Colonies on PDA, reaching 45–50 mm diameter after two weeks at 20–25 ℃, filamentous, filamentous margin, flat, mycelium embedded in the medium, sparse, white to gray.

 

Material examined: China, Lancang, Lahu Autonomous Prefecture, Hani, on dead woody twigs of Cinnamomum glanuliferum (Lauraceae), 23 March 2020, G.C. Ren, W04 (HKAS 122772), living culture KUMCC 21-0667.

 

Known distribution: On bark and stem of Buxus sempervirens (Buxaceae) in Germany (Thambugala et al. 2014), dead woody twigs of Cinnamomum glanuliferum (Lauraceae) in China (This study).

GenBank numbers: LSU: OQ170850, ITS: OQ158930, SSU: OQ168206 tef1-α: OR613412.

 

Notes: Our new collection groups with two strains of Stylodothis puccinioides (CBS 193.58 and CBS 194.58), isolated from Buxus sempervirens in Germany (Lumbsch et al. 2005) with 100% ML bootstrap support and 1.00 BYPP value (Fig. 5). The new collection shares similar morphology with the type material of Stylodothis puccinioides (PC 0084648) in having black, coriaceous, multiloculate ascostromata, bitunicate, 4-spored, cylindrical-clavate asci, uniseriate, ellipsoidal, brown, 1-septate ascospores with thick and smooth-walled (Thambugala et al. 2014). DNA sequences of Stylodothis puccinioides (CBS 193.58) differ in 4 nucleotides in the tef1-α region (0.4%, no gaps), while LSU and ITS sequences were identical. Hence, the new isolate is described as the first record of S. puccinioides from Cinnamomum glanuliferum (Lauraceae) in China.

 

 

Figure 1. Phylogram generated from ML analysis based on SSU, LSU, ITS, and tef1-α sequence data representing the family Dothideaceae. Related sequences are obtained following Gao et al. (2021). Seventy-three strains are included in the combined analyses, which comprise 3,269 characters for SSU, LSU, ITS, and tef1-α alignment. Pseudoseptoria collariana (CBS 135104) and P. obscura (CBS 135103) were used as the outgroup taxa. The best-scoring RAxML tree with a

 

 

Figure 2. Stylodothis puccinioides (HKAS 122772). a Material examined. b Appearance of ascostromata on the host substrate. c, d Sections of an ascostromata. e Peridium. f–i Asci. g–n Ascospores. o, p Culture characters on PDA (o = from above, p = from below). Scale bars: c, d = 150 μm, e = 100 μm, f–i = 50 μm, g–n = 10 μm, o, p = 30 mm.

 

References

1. Ren GC, Jayasiri SC, Tibpromma S, De Farias ARG, et al. (2024) Saprobic ascomycetes associated with woody litter from the Greater Mekong Subregion (Southwestern China and Northern Thailand). Mycosphere 15(1), 954–1082, Doi 10.5943/mycosphere/15/1/8

 

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