Distoseptispora obpyriformis
Distoseptispora obpyriformis Z.L. Luo & Hong Y. Su, in Luo, Hyde, Liu, Bhat, Bao, Li & Su, Mycosphere 9(3): 452 (2018)
Index Fungorum number: IF554290; Facesofungi number: FoF04194
Saprobic on submerged decaying wood in a freshwater stream. Anamorph: Colonies on wood effuse, hairy, dark brown, glistening, solitary or in small group. Mycelium immersed, composed of septate, pale brown to brown hyphae, smooth-walled. Conidiophores (42–)66–103(–115) × 5–6 µm (x̄ = 84 × 6 µm, n = 20), macronematous, mononematous, solitary or in groups, erect, straight or slightly flexuous, cylindrical, unbranched, 4–6-septate, brown, rounded at the apex, slightly enlarged at the base, smooth-walled. Conidiogenous cells (10–)15–22(–25) × 5–6 µm (x̄ = 19 × 5 µm, n = 20), monoblastic, terminal, determinate, subcylindrical, brown, smooth-walled. Conidia (55–)69–126(–168) × 10–12 µm (x̄ = 98 × 11 µm, n = 25), acrogenous, solitary, obclavate, olivaceous to pale or dark brown, truncate at base, tapering towards the apex, straight or slightly curved, constricted at the septa, 7–18-distoseptate, guttulate, thick and smooth-walled. Teleomorph: Undetermined.
Culture characteristics: Conidia germinating on PDA within 12 hrs and germ tubes produced from apex and septa of conidium. Colonies growing on PDA reaching 4–5 cm in 20 days at 26 °C in the dark, with dense, velvety, middle papillae, pale to dark brown mycelium from above; dark brown from below.
Material examined: China, Yunnan Province, Dali City, Weishan Yi and Hui Autonomous County, 25°29′31"N, 100°06′56"E, on submerged decaying branches in a freshwater stream, 19 February 2022, Z.Q. Zhang & Q.X. Yang YJ 1–19–1 (HKAS 125823, living culture KUNCC 23–13047).
Notes: Phylogenetic analysis revealed that our new strain KUNCC 23–13047 clustered with two strains of Distoseptispora obpyriformis (MFLUCC 17–1694 (ex-type strain) and DLUCC 0867). A comparison of the LSU, tef1-α and rpb2 gene of type strains between KUNCC 23–13047 and MFLUCC 17–1694 (from holotype) revealed 0% (0/1215 bp), 0.37% (3/812 bp, including 1 gaps), 0% (0/838 bp), 0.29% (3/1034 bp), respectively. Although our collection differs significantly in conidia size from the original description of D.obpyriformis (Luo et al. 2018a), multigene sequence data do not support this collection as a separate species. Similar results have been reported in previous studies and were found in several species in this study (Yang et al. 2018; Shen et al. 2021; Ma et al. 2022). Therefore, a new additional record of D.obpyriformis is reported from the Red River Basin in Yunnan, China.
Figure 1. Maximum likelihood (ML) tree is based on combined LSU, ITS, tef1-α and rpb2 sequence data. Bootstrap support values with a ML greater than 65% and Bayesian posterior probabilities (PP) greater than 0.95 are given above the nodes, shown as “ML/PP”. The tree is rooted to Aquapteridospora fusiformis (MFLUCC 18–1606) and A.lignicola (MFLUCC 15–0377). New species are indicated in blue and type strains are in bold.
Figure 2. Distoseptispora obpyriformis (HKAS 125823) a, b colonies on woody substrates c conidiophores d, e conidiophores with conidia f conidiogenous cell g–j conidia k germinating conidium l culture on PDA. Scale bars: 30 μm (c–e, g–k); 20 μm (j).
References
Shen H-W, Bao D-F, Boonmee S, Lu Y-Z, Su X-J, Li Y-X, Luo Z-L (2024) Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China. MycoKeys 102: 1–28. https://doi.org/10.3897/mycokeys.102.116096
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