Diaporthe melongenicola
Diaporthe melongenicola Fallahi, Jayaward. & K.D. Hyde, in Fallahi, Armand, Al-Otibi, Hyde & Jayawardena, MycoKeys 117: 224 (2025)
Index Fungorum number: IF903266 Facesoffungi number:
Pathogenic to makhuea (Solanum melongena) and causes blight, stem cankers, and fruit rot. Sexual morph not observed. Conidiomata pycnidial, superficial to embedded, solitary to aggregated, with single or multiple cavities, globose, with cream conidial mass, up to 200 μm in diameter. Conidiophores hyaline, smooth, straight, unbranched, rounded at the tip, and wider at the base, septate, 9–20 × 1.2–2.9 μm (mean = 13.4 × 1.9 μm, n = 15). Paraphyses intermingled among conidiophores, hyaline, smooth, septate, 9–33 × 1.4–1.5 μm (mean = 24 × 1.16 μm, n = 15). Beta conidia filiform, curved at one tip, hyaline, aseptate, rounded at tips, 15–27 × 1–2 μm (mean = 20 × 1.5 μm, n = 30). Alpha and gamma conidia are absent.
Culture characteristics: Colonies on PDA reach 70–80 mm in diameter after seven days of growth at 25 °C in the dark, fluffy and slightly felted with age, circular in shape, with cottony growth of the aerial mycelium in rings, grey to olivaceous grey, pale brown with age. The reverse is reddish brown, with many black dots.
Material examined: Thailand, Chiang Rai, Mueang Chiang Rai District, Doi Hang, stem canker in makhuea (Solanum melongena), January 2023, Maryam Fallahi, dried culture MF90-2 (MFLU 24-0244, holotype), ex-holotype culture, MFLUCC 24-0242.
Notes: In the phylogenetic tree generated in this study, strain MFLUCC 24-0242 formed a distinct branch within the D. sojae species complex in the subclade that includes the type strains of D. morindendophytica (ZHKUCC 22-0069, holotype) and D. tectonendophytica (MFLUCC 13-0471, ex-type) with 89% ML bootstrap support and 0.90 BYPP and is introduced here as a new species, namely Diaporthe melongenicola. The base pair differences between Diaporthe melongenicola (MFLUCC 24-0242) with type strains of closely related species are presented in Table 4. A pairwise homoplasy index (PHI) test indicated no significant recombination (Φw = 1.0) between D. melongenicola (MFLUCC 24-0242, holotype) and its closely related taxa (Fig. 19). These species are different from our isolate by producing alpha conidia and diverse culture characteristics. Considering both morphology and molecular data, D. melongenicola (MFLUCC 24-0242) could be identified as a novel species on makhuea (Solanum melongena) in northern Thailand.
Figure 1. Phylogenetic tree of the Diaporthe sojae species complex generated by maximum likelihood of combined ITS, tef1, tub2, cal, and his3 sequence data. The ultrafast maximum likelihood (ML) bootstrap support values ≥50% (BT) and Bayesian posterior probabilities ≥0.90 (BYPP) are shown, respectively, near the nodes. The ex-type strains are marked with an asterisk. The tree is rooted in D. amygdali (CBS 126679) and D. amygdali (CBS 115620).
Figure 1. Continued.
Figure 2. Diaporthe melongenicola (MFLUCC 24-0242, holotype) a blight and stem canker in makhuea b front, and c back view of the colony on PDA after 10 days d front and e back view of the colony on PDA after 60 days f–h conidiomata i, j section view of conidiomata k, l conidiophores and paraphyses m beta conidia. Scale bars: 20 μm (i, j); 10 μm (k–m).
References
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