Diaporthales » Cryphonectriaceae » Eriocamporesia

Eriocamporesia aurantia

Eriocamporesia aurantia R.H. Perera, Samarak. & K.D. Hyde, in Hyde et al., Fungal Diversity 100: 165 (2020)

Index Fungorum number: IF556792         Facesoffungi number: FoF 06960

 

Associated with twigs of dicotyledonous plant. Sexual morph: Ascostromata on host single, appearing as yellow pustules, 450–670 μm high, 870–1000 μm diam., pulvinate, semi-immersed in plant tissue, orange, KOH + purple; ascostromatic tissue pseudoparenchymatous, covering the top of the ascomatal bases. Ascomata 450–700 μm high, 120–330 μm diam., perithecial, usually valsoid, embedded in stromata at irregular levels, fuscous black, necks emerge at stromatal surface as dark orange brown ostioles covered with orange stromatal tissue to form papillae extending above stro- matal surface. Peridium 12–24 μm wide, composed of 5–7 layers of flattened, elongate, brown-walled, cells of textura angularis. Paraphyses lacking. Asci 30–43 × 6.7–8.7 µm ( = 37.6 × 7.8, n = 14), 8-spored, unitunicate, oblong ellipsoidal to fusoid to ellipsoidal, apedicellate, floating freely in the perithecial cavity, thin-walled, apex simple. Ascospores 9.5–14.5 × 2.5–3.3 μm ( = 11.6 × 2.8, n = 30),1-seriate, cylindrical to allantoid, occasionally ellipsoidal, ends round, curved or sometimes straight, aseptate, hyaline, smooth-walled, without appendages. Asexual morph: Conidiostromata 445–630 μm high, 1500–1800 μm diam., appearing as yellow pustules on the host, occur as separate structures, pulvinate, semi-immersed, orange. Conidiomata multi-locular, with locules often convoluted, apapillate, with 1-ostiolar opening, orange, KOH + purple; stromatic tis- sue pseudoparenchymatous. Paraphyses lacking. Pycnidial walls 4–7 μm wide, composed of 2–4 layers of flattened, elongate, hyaline, cells of textura angularis. Conidiophores 16.3–23.6 μm long, 1.6–2.3 wide, cylindrical, rarely septate or branching, hyaline. Conidiogenous cells 8.8–9.7 μm long, 1.1–1.6 wide, phialidic, cylindrical with attenuated apices, hyaline. Conidia 3.7–5.7 × 1–1.5 μm (x = 4.9 × 1.2, n = 30), hyaline, cylindrical or allantoid, ends round, aseptate, hyaline, smooth-walled, without appendages.

 

Culture characteristics: Ascospores germinated on PDA within 12 h, colonies on PDA reaching 35–38 mm diam. after 2 weeks at 25 °C, mycelium partly superficial, partly immersed, slightly effuse, with irregular edge, initially white, becoming orangish with time.

 

Host and habitat: On twigs of dicotyledonous plant (Hyde et al. 2020)

 

Known host and distribution: Thailand

 

Material examined: THAILAND, Chiang Mai Province, Mae Rim District, on twigs of dicotyledonous plant, R.H. Perera, 9 September 2017, Rim19 (MFLU 19-0943, holotype), ex-type living culture, MFLUCC 17-2625.

 

GenBank Accession No: ITS: MN699135, LSU: MN699130.

 

Notes: Eriocamporesia aurantia clusters within Cryphonectriaceae as a separate lineage. BLASTn search of the ITS rDNA region of new isolate showed the highest similarity to Amphilogia gyrosa (Berk. & Broome) Gryzenh., H.F. Glen & M.J. Wingf. strain 91123101 (94% similarity, 465/494 bp), Endothia gyrosa (Berk. & Broome) Höhn. strain AFTOL-ID 1223 (94% similarity, 531/564 bp), E. radicalis (Schwein. ex Fr.) Tul. & C. Tul. strain CBS116.13 (94% similarity, 549/585 bp), Chrysoporthe inopina Gryzenh. & M.J. Wingf. strain CMW 12731 (94% similarity, 549/585 bp) and strain CBS 116.13 (94% similarity, 543/580 bp). Eriocamporesia aurantia shares similar asexual morph characters to Amphilogia (Gryzenhout et al. 2005; Senanayake et al. 2017). However, it can be distinguished from Amphilogia species in having valsoid perithecia, and aseptate, cylindrical to allantoid ascospores, while Amphilogia has diatrypoid perithecia, and 1–3-septate, fusoid to ellipsoid ascospores (Gryzenhout et al. 2005; Senanayake et al. 2017). Considering both molecular and morphological data, we introduce E. aurantia as a novel species.

 

 

 

 

Figure X. Sexual morph of Eriocamporesia aurantia (MFLU 19-0943, holotype). a Herbarium material. b Ascostromata on host. c Longitu- dinal section of ascostroma. d Section of peridium. e Peridium in face view. f–j Asci. k–o Ascospores. p Germinating ascospore. Scale bars: b, c = 200 μm, d–j = 20 μm, k–o = 10 μm, p = 20 μm.