Chaetosphaeriales » Chaetosphaeriaceae » Thozetella

Thozetella lithocarpi

Thozetella lithocarpi R.H. Perera & K.D. Hyde, in Phookamsak et al., Fungal Diversity: 10.1007/s13225-019-00421-w, [147] (2019)

Index Fungorum number: IF555300         Facesoffungi number: FoF04923

 

Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies effuse, superficial, sessile sporodochial, greenish white. Sporodochia subulate, scattered, sessile, of greenish white mass, with a white spore mass at the apex. Microawns 24–54 µm long, 3–3.5 µm wide, visible as small hairs on the

sporodochial mass, aseptate, variously-shaped, sigmoid or sickle-shaped, apex straight, hyaline, smooth-walled, thickwalled. Conidiophores 21–30 × 1.5–3.5 µm, macronematous, packed in a bundle, usually ramose. Conidia 20–35 × 2–3 µm (x̄ = 26 × 2.7 µm, n = 25), falcate, inequilateral, truncate at both ends, hyaline, smooth-walled, with a single filiform setula at each end, 6.9–8.7 µm long.

 

Culture characteristics: Colonies on MEA reaching 35 mm diam. after 3 weeks, margins effuse, grey to dark brown, flat, lacking aerial mycelium, reverse dark brown to black.

 

Material examined: THAILAND, Chiang Mai Province, on a dried seed of Lithocarpus sp. (Fagaceae; 19 species of Lithocarpus have been reported from Chiang Mai Province, according to BGO plant database), 22 December 2015, R.H. Perera, S-7 (MFLU 16–1068, holotype), extype living culture, MFLUCC 16–0194.

 

GenBank Accession No: ITS = MH810433, LSU = MH810432.

 

Notes: Thozetella lithocarpi (MFLUCC 16–0194) shows a close relationship with T. pandanicola Tibpromma & K.D. Hyde, T. pinicola S.Y. Yeung et al. and T. nivea (Berk.) Kuntze. However, T. lithocarpi can be distinguished from T. pandanicola and T. pinicola in having larger conidia and presence of microawns (Jeewon et al. 2009; Tibpromma et al. 2018). Thozetella lithocarpi produces microawns with a straight apex, while they are undulating to geniculate in T. nivea (Barbosa et al. 2011).

A comparison of nucleotides in ITS gene region shows that T. lithocarpi differs from T. pandanicola and T. pinicola by 9 nucleotides (1.9%) and T. nivea by 18 nucleotides (3.9%). Our new species is also compared with Thozetella species that lack molecular data. Thozetella lithocarpi produces comparatively larger conidia (20–35 × 2–3 µm versus 11–17 × 2–2.5 µm) and smaller microawns (24–54 µm long, 3–3.5 µm wide versus 40–100 µm long, 2.5–4 µm wide) than T. cubensis R.F. Castan˜eda & G.R.W. Arnold (Castan˜eda-Ruiz and Arnold 1985). Thozetella canadensis Nag Raj produces verrucose microawns, while T. lithocarpi produces smooth-walled microawns (Nag Raj 1976). Thozetella aculeata Prisc. Silva & Grandi, T. buxifolia Allegr. et al., T. effusa B. Sutton & G.T. Cole, T.

radicata (E.F. Morris) Piroz. & Hodges, T. serrata Whitton et al., T. submersa F.R. Barbosa & Gusma˜o and T. tocklaiensis (Agnihothr.) Piroz. & Hodges also produces smaller conidia than those of T. lithocarpi. Thozetella ypsiloidea J.S. Monteiro et al. differs from T. lithocarpi by its Y-shaped microawns. Microawns of T. serrata have a serrated edge, while microawns have a straight apex in T. lithocarpi (Monteiro et al. 2016).

 

 

 

Figure 12. Thozetella lithocarpi (MFLU 16-1068, holotype). a Dried seed of Lithocarpus sp. b, c Appearance of conidiomata on host substrate. d–f Conidiophores with conidia. g–i Microawns. j–l Conidia. m Germinating conidium. Scale bars b = 1 mm, c = 500 µm, d–i = 20 µm, j–m = 10 µm

 

 

Reference:

 

Phookamsak R, Hyde KD, Jeewon R, Bhat DJ (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 95:1–273.

 

 

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