Chaetosphaeriales » Chaetosphaeriaceae » Sporoschisma

Sporoschisma chiangraiense

Sporoschisma chiangraiense N.G. Liu & K.D. Hyde, Fungal Diversity 96: 160 (2019).

Index Fungorum number: IF555386         Faceoffungi number: FoF04950

 

Saprobic on submerged decaying wood. Sexual morph: Undetermined. Asexual morph: Colonies on natural substrate effuse, black, hairy, with long chains of conidia. Mycelium immersed, composed of hyaline, guttulate, branched, 1.5–3 wide hyphae. Setae 85–150 × 3.5–8 μm, scattered or in groups mixed with conidiophores, capitate, sometimes surrounded by hyaline mucilage at the swollen apex, smooth, median brown, paler towards the apex, straight or flexuous, septate. Conidiophores 100–220 μm long, 5–8 μm wide below venter, 11–13.5 μm wide above, and 13–16 μm wide at venter, macronematous, mononematous, smooth, dark brown to black, paler at the torn apex, straight or slightly flexuous, solitary or in groups of 2–3, arising from dark brown to black bulbous base, composed of a cylindrical stipe and a swollen venter with a long cylindrical neck, erect, sometimes proliferating percurrently. Conidiogenous cells monophialidic, percurrent, integrated, brown, lageniform, frayed at the apex. Conidia 15.5–30 × 8.5–12.5 μm (x̄  = 23.8 × 10.7 μm, n = 30), formed in chains, cylindrical, aseptate, hyaline, verruculose, smooth-walled, with a conspicuous guttule at centre when young, 1-septate, brown, with conspicuous, circular guttules in each cell when mature, conspicuously darkened and slightly constricted at the septa, thickened at both ends

 

Culture characteristics: Conidia germinating on water agar media within 48 h. One or two germ tubes produced randomly from conidium wall. Colonies growing on PDA white, irregular, circular, edge entire, mycelium fluffy, dense in the centre, becoming sparse at the edge.

 

Habitat: on decaying wood submerged in a freshwater stream

 

Known distribution: Thailand

 

Material examined: THAILAND, Chiang Rai Province, Muang District, Ban Nang Lae Nai, on decaying wood submerged in a freshwater stream, 6 March 2018, N.G. Liu, CR066 (MFLU 18-1722, holotype), ex-type living culture (MFLUCC 18-0703).

 

GenBank Accession No: ITS: MH883032, LSU: MH883030, SSU: MH883048.

 

Notes: Sporoschisma chiangraiense is phylogenetically related to S. aquaticum and S. palauense. Comparisons of ITS sequences showed that there are 106 bp differences with gaps of total 558 bp between S. chiangraiense and S. aquaticum, and 58 bp differences with gaps of total 563 bp between S. chiangraiense and S. palauense. Sporoschisma chiangraiense differs from S. aquaticum and S. palauense in having 1-septate conidia when mature, while the latter two are 3-septate, and 1–3-septate respectively. Conidia of S. chiangraiense have conspicuous guttules, a feature absent in S. aquaticum.